TITLE

Saccharin consumption increases sperm DNA fragmentation and apoptosis in mice

AUTHOR(S)
Rahimipour, Marzieh; Talebi, Ali Reza; Anvari, Morteza; Sarcheshmeh, Abolghasem Abbasi; Omidi, Marjan
PUB. DATE
May 2014
SOURCE
Iranian Journal of Reproductive Medicine;May2014, Vol. 12 Issue 5, p307
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Saccharin is an artificial non-caloric sweetener that used to sweeten products such as drinks, candies, medicines, and toothpaste, but our bodies cannot metabolize it. Sodium saccharin is considered as an important factor in tumor promotion in male rats but not in humans. Objective: The objective of this study was to investigate the effect of saccharin consumption on sperm parameters and apoptosis in adult mice. Materials and Methods: Totally 14 adult male mice were divided into 2 groups. Group 1 served as control fed on basal diet and group 2 or experimental animals received distilled water containing saccharin (0.2% w/v) for 35 days. After that, the left cauda epididymis of each mouse was cut and placed in Ham's F10. Swimmedout spermatozoa were used to analyze count, motility, morphology (Pap-staining) and viability (eosin-Y staining). Sperm DNA integrity, as an indicator of apoptosis, was assessed by SCD (sperm chromatin dispersion) and terminal deoxynucleotidyl transferase (TUNEL) assay. Results: Following saccharin consumption, we had a reduction in sperm motility with respect to control animals (p=0.000). In addition, the sperm count diminished (17.70±1.11 in controls vs. 12.80±2.79 in case group, p=0.003) and the rate of sperm normal morphology decreased from 77.00±6.40 in control animals into 63.85±6.81 in saccharin-treated mice (p=0.001). Also, we saw a statistically significant increase in rates of sperm DNA damage and apoptosis in experimental group when compared to control one (p=0.001, p=0.002 respectively). Conclusion: Saccharin consumption may have negative effects on sperm parameters, and increases the rate of sperm DNA fragmentation and apoptosis in mice.
ACCESSION #
97301802

 

Related Articles

  • Testicular germ cell apoptosis and sperm defects in mice upon long‐term high fat diet feeding. Ghosh, Songita; Mukherjee, Sutapa // Journal of Cellular Physiology;Oct2018, Vol. 233 Issue 10, p6896 

    The growing prevalence of male infertility is a matter of serious concern. One of the putative causes being nutritional excess from continuous consumption of high fat diet (HFD) leading to insulin resistance albeit the specific relationship is not fully understood. Besides, there are many...

  • Saccharin consumption increases sperm apoptosis in adult mice. Rahimipour, M.; Talebi, A. R.; Omidi, M. // Iranian Journal of Reproductive Medicine;AprSupp, p61 

    Introduction: Saccharin is an artificial non-caloric sweetener that first synthesized in 1879. It is used to sweeten products such as drinks, candies, biscuits, medicines, and toothpaste whereas our bodies cannot metabolize it. Sodium saccharin is considered as an important factor in tumor...

  • Changes in saccharin preference behavior as a primary outcome to evaluate pain and analgesia in acetic acid-induced visceral pain in mice. de la Puente, Beatriz; Romero-Alejo, Elizabeth; Vela, José Miguel; Merlos, Manuel; Zamanillo, Daniel; Portillo-Salido, Enrique // Journal of Pain Research;2015, Vol. 8, p663 

    Reflex-based procedures are important measures in preclinical pain studies that evaluate stimulated behaviors. These procedures, however, are insufficient to capture the complexity of the pain experience, which is often associated with the depression of several innate behaviors. While recent...

  • Epididymis Response Partly Compensates for Spermatozoa Oxidative Defects in snGPx4 and GPx5 Double Mutant Mice. Noblanc, Anaïs; Peltier, Manon; Damon-Soubeyrand, Christelle; Kerchkove, Nicolas; Chabory, Eléonore; Vernet, Patrick; Saez, Fabrice; Cadet, Rémi; Janny, Laurent; Pons-Rejraji, Hanae; Conrad, Marcus; Drevet, Joël R.; Kocer, Ayhan // PLoS ONE;Jun2012, Vol. 7 Issue 6, p1 

    We report here that spermatozoa of mice lacking both the sperm nucleaus glutathione peroxidase 4 (snGPx4) and the epididymal glutathione peroxidase 5 (GPx5) activities display sperm nucleus structural abnormalities including delayed and defective nuclear compaction, nuclear instability and DNA...

  • 53BP1 Mediates the Fusion of Mammalian Telomeres Rendered Dysfunctional by DNA-PKcs Loss or Inhibition. Rybanska-Spaeder, Ivana; Ghosh, Rajib; Franco, Sonia // PLoS ONE;Sep2014, Vol. 9 Issue 9, p1 

    Telomere dysfunction promotes genomic instability and carcinogenesis via inappropriate end-to-end chromosomal rearrangements, or telomere fusions. Previous work indicates that the DNA Damage Response (DDR) factor 53BP1 promotes the fusion of telomeres rendered dysfunctional by loss of TRF2, but...

  • GADD45β plays a protective role in acute lung injury by regulating apoptosis in experimental sepsis in vivo. Moon, Sung‐Je; Kim, Hyun‐Yong; Kim, Yong‐Hoon; Kim, Kyoung‐Shim; Noh, Jung‐Ran; Kim, Hyun‐Jong; Choi, Ji‐Hyun; Hwang, Jung H.; Lee, Chul‐Ho // Journal of Cellular Physiology;Oct2018, Vol. 233 Issue 10, p7128 

    Sepsis is a systemic inflammatory response syndrome due to microbial infection. Growth arrest and DNA‐damage‐inducible 45 beta (GADD45β) are induced by genotoxic stress and inflammatory cytokines. However, the role of GADD45β during bacterial infection remains unclear....

  • In vivo single-photon emission computed tomography imaging of apoptosis in Crohn's disease and anti-tumour necrosis factor therapy. Watson, Alastair J. M. // Gut;Apr2007, Vol. 56 Issue 4, p461 

    The article reports on a study by J. M. H. Van der Brande and colleagues on evidence for the importance of apoptosis of lamina propria T cells in the action of infliximab. The researchers demonstrate that apoptosis in the intestine is increased compared with uninflammed intestine in two mouse...

  • Galectin-9 in Allergic Airway Inflammation and Hyper-Responsiveness in Mice. Sziksz, Erna; Kozma, Gergely Tibor; Pállinger, Éva; Komlósi, Zsolt István; Ádori, Csaba; Kovács, Lajos; Szebeni, Beáta; Rusai, Krisztina; Losonczy, György; Szabó, András; Vannay, Ádám // International Archives of Allergy & Immunology;2010, Vol. 151 Issue 4, p308 

    Background: Galectin-9 (Gal-9) is a member of the growing family of β-galactoside-binding lectins. Gal-9 is an eosinophil chemoattractant and inducer of Th1 cell apoptosis. These effects suggest its potential role in the pathogenesis of asthma. Our aim was to study the expression of Gal-9 in...

  • Baicalin Improves Survival in a Murine Model of Polymicrobial Sepsis via Suppressing Inflammatory Response and Lymphocyte Apoptosis. Jiali Zhu; Jiafeng Wang; Ying Sheng; Yun Zou; Lulong Bo; Fei Wang; Jingsheng Lou; Xiaohua Fan; Rui Bao; Youping Wu; Feng Chen; Xiaoming Deng; Jinbao Li // PLoS ONE;May2012, Vol. 7 Issue 5, p1 

    Background: An imbalance between overwhelming inflammation and lymphocyte apoptosis is the main cause of high mortality in patients with sepsis. Baicalin, the main active ingredient of the Scutellaria root, exerts anti-inflammatory, antiapoptotic, and even antibacterial properties in...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics