Comparison of reactive oxygen species in neat and washed semen of infertile men

Moein, Mohammad Reza; Vahidi, Serajedin; Ghasemzadeh, Jalal; Tabibnejad, Nasirn
May 2014
Iranian Journal of Reproductive Medicine;May2014, Vol. 12 Issue 5, p301
Academic Journal
Background: Male are involved in near 50% of cases of infertility and reactive oxygen species (ROS) playing an important role in decreasing fertility potential. Accurate measurement of ROS seems to be important in evaluation of infertile male patients. Objective: To compare ROS measurement in neat and washed semen samples of infertile men and define the best method for evaluation of ROS in these patients. Materials and Methods: We measured the level of ROS in semen samples of thirty five non-azoospermic men with infertility. The semen samples were divided into two parts and the semen parameters and ROS levels in neat and washed samples were evaluated. We also evaluated the presence of pyospermia using peroxidase test. Results: The differences regarding sperm count and quick motility were significant in neat and washed semen samples. The mean ROS level was significantly higher in neat samples compared with washed spermatozoa (7.50 RLU vs. 1.20 RLU respectively). Difference in ROS levels was more significant in patients with pyospermia compared to whom with no pyospermia (378.67 RLU vs. 9.48 RLU respectively). Conclusion: Our study confirmed that neat or unprocessed samples are better index of normal oxidative status of semen samples. Because we do not artificially add or remove factors that may play an important role in oxidative equilibrium status.


Related Articles

  • Effect of Oxidative stress on sperm quality in Leukocytospermic infertile men. Vidya, Garg; Garg, S. P.; Rawekar, A. T.; Deshpande, V. K.; Biswas, D. A.; Sawane, M. V.; Akarte, A. N. // Biomedical Research (0970-938X);2011, Vol. 22 Issue 3, p329 

    It has been proposed that leukocytospermia has a role to play in male infertility. Leukocytes are considered to be the primary source for generation of reactive oxygen species (ROS) resulting in oxidative stress. The aim of this study was to evaluate the effect of oxidative stress created by...

  • Patients with Low Levels Have Seminal Oxidative Stress. Agarwal, Ashok // Fertility Weekly;5/18/2015, p8 

    The article presents a study which evaluates the effect of low-level leukocytospermia on semen quality, reactive oxygen species (ROS) levels, and DNA damage in male infertility. They explain that the condition has been categorized by the World Health Organization (WHO) guidelines as low-level...

  • Proteomic analysis of seminal fluid from men exhibiting oxidative stress. Sharma, Rakesh; Agarwal, Ashok; Mohanty, Gayatri; Du Plessis, Stefan S.; Gopalan, Banu; Willard, Belinda; Yadav, Satya P.; Sabanegh, Edmund // Reproductive Biology & Endocrinology;2013, Vol. 11 Issue 1, p1 

    Background: Seminal plasma serves as a natural reservoir of antioxidants. It helps to remove excessive formation of reactive oxygen species (ROS) and consequently, reduce oxidative stress. Proteomic profiling of seminal plasma proteins is important to understand the molecular mechanisms...

  • EFFECT OF VARIOUS BIOMOLECULES FOR NORMAL FUNCTIONING OF HUMAN SPERM FOR FERTILIZATION: A REVIEW. Vickram, A. S.; Ramesh Pathy, M.; Sridharan, T. B. // International Journal of Pharmacy & Pharmaceutical Sciences;Oct-Dec2012, Vol. 4 Issue 4, p18 

    Semen is a mixture of sperm and secretions of seminal vesicle, prostate gland and Cowper's gland. The molecules present in the semen includes Sperm, amino acids, proteins, lipids, Anti-sperm antibodies and trace elements like include zinc, megnesium, sodium and potasium. Each of these molecules...

  • Reactive oxygen species and sperm DNA damage in infertile men presenting with low level leukocytospermia. Agarwal, Ashok; Mulgund, Aditi; Alshahrani, Saad; Assidi, Mourad; Abuzenadah, Adel M.; Sharma, Rakesh; Sabanegh, Edmund // Reproductive Biology & Endocrinology;2014, Vol. 12 Issue 1, p66 

    Background Leukocytes contribute directly and indirectly to reactive oxygen species (ROS) production. Although leukocytospermia is defined as the presence of ≥1 × 10(6) white blood cells/mL (WBC/mL) in a semen sample, the presence of less than 1×10(6) WBC/mL (low-level...

  • Male Infertility. WATSON, LISA // Naturopathic Doctor News & Review;Aug2014, Vol. 10 Issue 8, p21 

    The article discusses naturopathic treatment options for male infertility. Diagnosis of the multifactorial condition is initiated with semen analysis based on World Health Organization (WHO) standards and followed with medical intervention in reproduction. Deterioration from reactive oxygen...

  • Clinical Significance and Expression of PAF and TNF-alpha in Seminal Plasma of Leukocytospermic Patients. Chaodong Liu; Hongjian Liu; Xianzhong Wang; Sun Xinbo // Mediators of Inflammation;2012, Vol. 2012, p1 

    Objective. Discuss the changes and roles of PAF in the reproductive tract infection by observing the expression of platelet activating factor (PAF) and tumor necrosis factor α (TNF-α) in seminal plasma of patients with leukocytospermia. Methods. The seminal plasma was obtained from 22...

  • Semen PH correlation to age, volume, viscosity and sperm motility. Sha'abani, K.; Nasirian, N. // Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p112 

    Introduction: Men infertility could have different causes. The first step in men infertility treatment is semen analysis. Different factors could analyze in semen experiment. One of the important factors is semen PH which the normal range of it is 7.2-8. Materials and Methods: In this study, 200...

  • Does Varicocelectomy Improve Gonadal Function in Men with Hypogonadism and Infertility? Analysis of a Prospective Study. Srini, Vasan Sathya; Veerachari, Srinivas Belur // International Journal of Endocrinology;2011, p1 

    Varicocele in infertile males is associated with Leydig cell dysfunction and hypogonadism. The effect of varicocelectomy on serum testosterone level is not yet established. We analysed 200 heterosexual infertile men diagnosed to have clinical varicocele they were divided into two groups: group 1...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics