TITLE

In silico characterization and comparative analysis of Bacillus subtilis GntR type LutR transcription factor

AUTHOR(S)
Avci, Murat Kemal; Yamaner, Cigdem; Ayvaz, Muavviz; Yazgan-Karatas, Ayten
PUB. DATE
January 2014
SOURCE
EurAsian Journal of Biosciences;2014, Vol. 8, p12
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: The GntR-type transcriptional factor LutR (formerly YvfI) behaves as a transition state regulator, governing adaptations of Bacillus subtilis cells to the transition from exponential growth to stationary phase. Material and Methods: In this study, we evaluated a total of 30 LutR proteins from different bacterial species that were available in the NCBI database. By performing the physicochemical analyses, domain analysis, and phylogenetic tree construction, we identified some similarities and differences among these 30 LutR proteins. Furthermore, only the primer structure of Bacillus subtilis 168 LutR was compared with the sequences and 3D conformational situations of the well-known HTH-type transcriptional factors FadR (PDB ID: 1HW1, 1HW2, 1HT9) and YvoA (PDB ID: 2WV0) using PyMOL. Results: These analyses revealed that the critical residues for DNA-recognition and DNA-binding of LutR are highly conserved and conformationally correspond to the same positions as those in FadR and YvoA. The sequence (15-SVQALAESF-23) of the second helix in LutR seems to be important for DNA-binding, while the Q17-R27-Q47 residues might be critical for DNA recognition. Here, we provide a detailed description of the similarities and differences between B. subtilis LutR and both the other LutR proteins from different bacterial species and other HTH-type transcriptional factors. Conclusions: These results provide a scientific groundworkbase and can be used for advanced in silico analysis, homology modelling, and in vitro studies, including DNA-protein interaction analysis, such as ChIP and EMSA methods.
ACCESSION #
97173685

 

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