TITLE

DNA adducts, detected by [sup 32]P postlabelling, in human cholangiocarcinoma

AUTHOR(S)
Khan, S.A.; Carmichael, P.L.; Taylor-Robinson, S.D.; Habib, N.; Thomas, H.C.
PUB. DATE
April 2003
SOURCE
Gut;Apr2003, Vol. 52 Issue 4, p586
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Reported mortality from intrahepatic cholangiocarcinoma (CCa) has risen steeply in the UK and other industrialised countries over the past 30 years, the cause of which has not been explained. DNA adduct formation is promutagenic and demonstrates exposure to a DNA damaging agent. It is a key step in chemically induced carcinogenesis. We hypothesise that the increase in CCa mortality is caused by a rise in a genotoxic environmental agent(s), causing cholangiocyte DNA damage. Aims: To investigate and compare tumour and tumour adjacent CCa tissue, and non-cancer control bile duct tissue, for DNA adducts as a biomarker of genotoxin exposure. Methods: DNA from 32 intrahepatic CCa patients (and in 28 cases DNA from adjacent non-tumour tissue) and from biliary ducts of seven non-cancer patients were investigated for the presence of DNA adducts using the nuclease P1 method of [sup 32]P postlabelling. DNA adduct levels (number of adducts/10[sup 8] nucleotides) were quantified. Results: There was no significant difference in relative adduct labellings (RALs) between tumour adjacent DNA (median 8.6, range 1.2-51.6) and CCa DNA (7.2, 1.8-48.4). However, RALs were significantly higher in DNA from cancer patients (tumour adjacent and CCa DNA) compared with noncancer patient DNA (2.9, 0.6-11.5; p=0.032, two tailed Mann-Whitney U test). Different adduct patterns were also seen in CCa compared with non-cancer patients. Conclusion: Quantitative and qualitative differences in adducts between cancer and non-cancer patients support the hypothesis that genotoxins may play a role in the development of intrahepatic CCa.
ACCESSION #
9704758

 

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