TITLE

Duodenal mucosal reductase in wild-type and Hfe knockout mice on iron adequate, iron deficient, and iron rich feeding

AUTHOR(S)
Simpson, R.J.; Debnam, E.; Beaumont, N.; Bahram, S.; Schümann, K.; Srai, S.K.S.
PUB. DATE
April 2003
SOURCE
Gut;Apr2003, Vol. 52 Issue 4, p510
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Genetic haemochromatosis is a common hereditary iron loading disorder in humans. The disease is associated with loss of function mutations in the HFE gene. This is thought to change iron stores via increased iron absorption. Aims: In this study we investigated how adaptation of mucosal reductase activity is engaged in this process and how the changes compare with adaptation seen when an iron deficient diet is fed. Methods: Duodenal mucosal surface reductase was measured with nitroblue tetrazolium in age matched groups of male Hfe knockout mice (Hfe) and wild- type mice fed a purified diet containing normal (iron adequate), high (iron rich), or Iow (iron deficient) iron concentrations. Results: Reductase activity increased when mice were fed an iron deficient diet and decreased when they were fed an iron rich diet. Total villus activity, as measured by the average area under the activity curve along the crypt-villus axis, was increased 2.8-2.9-fold by iron deficiency in both genotypes. Approximately half of this difference was attributable to the significantly increased length of the villi in mice on an iron deficient diet (p<0.05). Hfe knockout did not affect villus length but increased mucosal reductase activity near the villus tips. Similar increases (1.3-1.6-fold) were seen on all diets but the increase was significant for iron deficient and iron loaded diets only (p<0.05). Conclusion: Hfe gene product and dietary iron downregulate villus reductase activity in mice.
ACCESSION #
9704743

 

Related Articles

  • Differential HFE Gene Expression Is Regulated by Alternative Splicing in Human Tissues. Martins, Rute; Silva, Bruno; Proença, Daniela; Faustino, Paula // PLoS ONE;2011, Vol. 6 Issue 3, p1 

    Background: The pathophysiology of HFE-derived Hereditary Hemochromatosis and the function of HFE protein in iron homeostasis remain uncertain. Also, the role of alternative splicing in HFE gene expression regulation and the possible function of the corresponding protein isoforms are still...

  • Iron overload.  // Mayo Clinic Health Letter;Jan1998, Vol. 16 Issue 1, p7 

    Presents information on hemochromatosis, the most common genetic disorder in the United States. Reasons for the occurrence of hemochromatosis; How hemochromatosis is inherited; Symptoms of hemochromatosis; Details on the transferrin saturation test; Information on the treatment required for...

  • Clinical and Biochemical Abnormalities in People Heterozygous for Hemochromatosis. Bulaj, Zaneta J.; Griffen, Linda M.; Jorde, Lynn B.; Edwards, Corwin Q.; Kushner, James P. // New England Journal of Medicine;12/12/96, Vol. 335 Issue 24, p1799 

    Background: Ten percent of whites are heterozygous for the HLA-linked hemochromatosis mutation. We performed a cross-sectional analysis of 1058 genotyped heterozygotes to define the effects of age and sex on the phenotype. Methods: The heterozygous genotype was assigned to 505 male and 553...

  • Hemochromatosis in Heterozygotes. Powell, Lawrie W.; Jazwinska, Elizabeth C. // New England Journal of Medicine;12/12/96, Vol. 335 Issue 24, p1837 

    Editorial. Discusses hemochromatosis. Why it has been much underdiagnosed and misdiagnosed; Comments on the work of Bulaj et al.; The observation that the expression of the disease was more marked in heterozygotes with paternally transmitted mutations; Comments on the work of Feder et al.; Why...

  • Heavy metal.  // People's Medical Society Newsletter;Dec96, Vol. 15 Issue 6, p1 

    Provides insights on hemochromatosis, a condition in which the body stores too much of the iron it absorbs from a normal diet. Effects of the disease; Causes; Underdiagnosed nature of the disease.

  • Hemochromatosis: Diagnosis and management. Little, David R. // American Family Physician;6/1/1996, Vol. 53 Issue 8, p2623 

    Presents a discussion on hemochromatosis. Pathophysiology; Epidemiology; Clinical presentation; Diagnosis; Treatment; Prognosis; Issue of screening for hemochromatosis.

  • Hemochromatosis: When your blood has too much iron.  // American Family Physician;6/1/1996, Vol. 53 Issue 8, p2631 

    Presents answers to questions concerning hemochromatosis. Symptoms of the illness; Diagnostic procedures; Treatment options; Dietary restrictions for people with the disease; Serious nature of the disease.

  • ERRATUM.  // Journal of Infectious Diseases;6/1/2008, Vol. 197 Issue 11, p1630 

    A correction to the article "Hemochromatosis gene polymorphisms, mitochrondrial haplogroups, and peripheral lipoatrophy during antiretroviral therapy" that was published in the March 15, 2008 issue is presented.

  • Genetic haemochromatosis. Hill, Barry // Independent Nurse;9/20/2010, p32 

    No abstract available.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics