TITLE

Identification and In Vitro Reactivity of Celiac Immunoactive Peptides in an Apparent Gluten-Free Beer

AUTHOR(S)
Real, Ana; Comino, Isabel; Moreno, Mª de Lourdes; López-Casado, Miguel Ángel; Lorite, Pedro; Torres, Mª Isabel; Cebolla, Ángel; Sousa, Carolina
PUB. DATE
June 2014
SOURCE
PLoS ONE;Jun2014, Vol. 9 Issue 6, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Gluten content from barley, rye, wheat and in certain oat varieties, must be avoid in individuals with celiac disease. In most of the Western countries, the level of gluten content in food to be considered as gluten-free products is below 20 parts per million measured by ELISA based on specific anti-gluten peptide antibody. However, in beverages or food suffering complex hydrolytic processes as beers, the relative proportion of reactive peptides for celiac patients and the analytical techniques may differ, because of the diversity of the resulting peptide populations after fermentations. A beer below 20 parts per million of gluten but yet detectable levels of gluten peptides by anti-gliadin 33-mer antibodies (G12 and A1) was analyzed. We identified and characterized the relevant peptides for either antibody recognition or immunoactivity in celiac patients. The beer was fractionated by HPLC. The relative reactivity of the different HPLC fractions to the G12/A1 antibodies correlated to the reactivity of peripheral blood mononuclear cells isolated from 14 celiac individuals. Peptides from representative fractions classified according to the relative reactivity to G12/A1 antibodies were identified by mass spectrometry. The beer peptides containing sequences with similarity to those of previously described G12 and A1 epitopes were synthesized and confirmed significant reactivity for the antibodies. The most reactive peptides for G12/A1 also confirmed the highest immunogenicity by peripheral blood mononuclear cell activation and interferon γ production from celiac patients. We concluded that preparative HPLC combined with anti-gliadin 33-mer G12/A1 antibodies were very sensitive and specific methods to analyze the relevant immunogenic peptides in hydrolyzed gluten.
ACCESSION #
96861059

 

Related Articles

  • Insights in the laboratory diagnosis of celiac disease. Basso, D.; Guariso, G.; Fogar, P.; Navaglia, F.; Zambon, C.-F.; Plebani, Mario // Lupus;2006, Vol. 15 Issue 7, p462 

    The present report focuses on the diagnosis of celiac disease and its pathogenesis, which depends on a genetic predisposition (HLA DQ2 or DQ8 haplotypes), gluten ingestion and T cell activation, type II transglutaminase (TG2), the autoantigen recognized by the antiendomysial antibody playing a...

  • Prevalence of celiac disease in patients with autoimmune thyroid disease. Sharma, Bharat; Varthakavi, P. K.; Chadha, M.; Bhagwat, N.; Joshi, A. S.; Lathia, T. B.; Mittal, S.; Sheikh, S.; Dholye, J.; Rahate, S. // Indian Journal of Endocrinology & Metabolism;Dec2012 Supplement, Vol. 16, pS516 

    Introduction: The prevalence of autoimmune thyroid disease (AITD) in India is 7 - 8% and the association of other autoimmune disorders with AITD is well documented. The concomitance of celiac disease (CD) with AITD results in poor absorption of thyroid medications and results in higher doses of...

  • Quantification of Hordeins by ELISA: The Correct Standard Makes a Magnitude of Difference. Tanner, Gregory J.; Blundell, Malcolm J.; Colgrave, Michelle L.; Howitt, Crispin A. // PLoS ONE;Feb2013, Vol. 8 Issue 2, p1 

    Background: Coeliacs require a life-long gluten-free diet supported by accurate measurement of gluten (hordein) in gluten-free food. The gluten-free food industry, with a value in excess of $6 billion in 2011, currently depends on two ELISA protocols calibrated against standards that may not be...

  • Measuring Hordein (Gluten) in Beer – A Comparison of ELISA and Mass Spectrometry. Tanner, Gregory J.; Colgrave, Michelle L.; Blundell, Malcolm J.; Goswami, Hareshwar P.; Howitt, Crispin A. // PLoS ONE;Feb2013, Vol. 8 Issue 2, p1 

    Background: Subjects suffering from coeliac disease, gluten allergy/intolerance must adopt a lifelong avoidance of gluten. Beer contains trace levels of hordeins (gluten) which are too high to be safely consumed by most coeliacs. Accurate measurement of trace hordeins by ELISA is problematic. ...

  • Cross-Reaction between Gliadin and Different Food and Tissue Antigens. Vojdani, Aristo; Tarash, Igal // Food & Nutrition Sciences;Jan2013, Vol. 4 Issue 1, p20 

    A subgroup of coeliac disease patients continues to experience symptoms even on a gluten-free diet (GFD). We attempted to determine whether these symptoms could be due to either cross-contamination with gluten-containing foods or cross-reactivity between α-gliadin and non-gluten foods...

  • A Non-Human Primate Model for Gluten Sensitivity. Bethune, Michael T.; Borda, Juan T.; Ribka, Erin; Liu, Michael-Xun; Phillippi-Falkenstein, Kathrine; Jandacek, Ronald J.; Doxiadis, Gaby G. M.; Gray, Gary M.; Khosla, Chaitan; Sestak, Karol // PLoS ONE;2008, Vol. 3 Issue 2, p1 

    Background and Aims: Gluten sensitivity is widespread among humans. For example, in celiac disease patients, an inflammatory response to dietary gluten leads to enteropathy, malabsorption, circulating antibodies against gluten and transglutaminase 2, and clinical symptoms such as diarrhea. There...

  • Non-invasive monitoring of celiac disease. Lakos, Gabriella // MLO: Medical Laboratory Observer;Dec2012, Vol. 44 Issue 12, p15 

    The article discusses Celiac disease (CD) and the non-invasive monitoring of CD. It discusses on the non-invasive follow-up and monitoring of CD through serological testing. It discusses about the gluten free diet (GFD) and states that the CD treatment including GFD has now more food options...

  • A limp in a pregnant woman as a first presentation of celiac disease. Jammah, A. // Osteoporosis International;Mar2012, Vol. 23 Issue 3, p1183 

    A 25-year-old Arab woman, reported to our endocrinology clinic one month post-partum presenting with back pain and a limp that started during the seventh month of pregnancy. Upon examination, she was found to have a full range of motion and no tenderness in the hip joint or lower back. The pain...

  • Importance of the Educational Environment in the Evolution of Celiac Disease. Samasca, Gabriel; Iancu, Mihaela; Pop, Tudor; Butnariu, Angela; Andreica, Mariana; Victor, Cristea; Dejica, Doru // Laboratory Medicine;Aug2011, Vol. 42 Issue 8, p497 

    Objective: We serologically monitored the evolution of patients with celiac disease under a gluten-free diet, in correlation with the family's level of education. Methods: Our study was performed in a representative sample of 50 children with celiac disease, in whom we monitored the evolution of...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics