Chondrogenesis of Infrapatellar Fat Pad Derived Adipose Stem Cells in 3D Printed Chitosan Scaffold

Ye, Ken; Felimban, Raed; Traianedes, Kathy; Moulton, Simon E.; Wallace, Gordon G.; Chung, Johnson; Quigley, Anita; Choong, Peter F. M.; Myers, Damian E.
June 2014
PLoS ONE;Jun2014, Vol. 9 Issue 6, p1
Academic Journal
Infrapatellar fat pad adipose stem cells (IPFP-ASCs) have been shown to harbor chondrogenic potential. When combined with 3D polymeric structures, the stem cells provide a source of stem cells to engineer 3D tissues for cartilage repair. In this study, we have shown human IPFP-ASCs seeded onto 3D printed chitosan scaffolds can undergo chondrogenesis using TGFβ3 and BMP6. By week 4, a pearlescent, cartilage-like matrix had formed that penetrated the top layers of the chitosan scaffold forming a ‘cap’ on the scaffold. Chondrocytic morphology showed typical cells encased in extracellular matrix which stained positively with toluidine blue. Immunohistochemistry demonstrated positive staining for collagen type II and cartilage proteoglycans, as well as collagen type I. Real time PCR analysis showed up-regulation of collagen type II, aggrecan and SOX9 genes when IPFP-ASCs were stimulated by TGFβ3 and BMP6. Thus, IPFP-ASCs can successfully undergo chondrogenesis using TGFβ3 and BMP6 and the cartilage-like tissue that forms on the surface of 3D-printed chitosan scaffold may prove useful as an osteochondral graft.


Related Articles

  • Extracellular matrix production in vitro in cartilage tissue engineering. Jie-Lin Chen; Li Duan; Weimin Zhu; Jianyi Xiong; Daping Wang // Journal of Translational Medicine;2014, Vol. 12 Issue 1, p1 

    Cartilage tissue engineering is arising as a technique for the repair of cartilage lesions in clinical applications. However, fibrocartilage formation weakened the mechanical functions of the articular, which compromises the clinical outcomes. Due to the low proliferation ability,...

  • Combining Innovative Bioink and Low Cell Density for the Production of 3D-Bioprinted Cartilage Substitutes: A Pilot Study. Henrionnet, Christel; Pourchet, Léa; Neybecker, Paul; Messaoudi, Océane; Gillet, Pierre; Loeuille, Damien; Mainard, Didier; Marquette, Christophe; Pinzano, Astrid // Stem Cells International;1/21/2020, p1 

    3D bioprinting offers interesting opportunities for 3D tissue printing by providing living cells with appropriate scaffolds with a dedicated structure. Biological advances in bioinks are currently promising for cell encapsulation, particularly that of mesenchymal stem cells (MSCs). We present...

  • HES1 and HES5 Are Dispensable for Cartilage and Endochondral Bone Formation. Karlsson, C.; Brantsing, C.; Kageyama, R.; Lindahl, A. // Cells Tissues Organs;2010, Vol. 192 Issue 1, p17 

    Notch signalling, via its downstream mediators HES1 and HES5, regulates development of several different tissues. In vitro studies suggest that these genes are also involved in chondrogenesis and endochondral bone formation. In order to investigate the importance of HES1 and HES5 for these...

  • Time-Dependent Processes in Stem Cell-Based Tissue Engineering of Articular Cartilage. Gadjanski, Ivana; Spiller, Kara; Vunjak-Novakovic, Gordana // Stem Cell Reviews & Reports;Sep2012, Vol. 8 Issue 3, p863 

    Articular cartilage (AC), situated in diarthrodial joints at the end of the long bones, is composed of a single cell type (chondrocytes) embedded in dense extracellular matrix comprised of collagens and proteoglycans. AC is avascular and alymphatic and is not innervated. At first glance, such a...

  • Effect of Transforming Growth Factor-ß3 and Bone Morphogenetic Protein-6 Growth Factors on Chondrogenic Differentiation of Adipose-Derived Stem Cells in Alginate Scaffold. Hashemibeni, Batool; Razavi, Shahnaz; Esfandiary, Ebrahim; Karbasi, Saeed; Mardani, Mohammad; Sadeghi, Farzane; Esfahani, Mohammadhosein Nasre; Nadali, Fatemeh; Shafiezade, Hamid // Journal of Isfahan Medical School;Dec2010, Vol. 28 Issue 112, p620 

    Background: Cartilage damages and diseases such as osteoarthritis are rather worldwide problems of many people. In addition, articular cartilage has a limited ability to repair. Current treatment methods for cartilage tissue injuries lead to formation of fibrous tissue, apoptosis, and further...

  • Effect of 3D Chondrocyte Culturing Conditions on the Formation of Extracellular Matrix in Cartilage Tissue-Engineering Constructs. Ponomarev, I.; Kochneva, L.; Barnewitz, D. // Bulletin of Experimental Biology & Medicine;Feb2014, Vol. 156 Issue 4, p548 

    We studied biochemical, morphological, and histological parameters of the extracellular matrix in scaffold-free tissue engineering chondrotransplants prepared from chondrocytes isolated from knee joint cartilage biopsy specimens of Haflinger horses (age 3.5-14 years) and in transplants prepared...

  • In vitro cartilage formation of composites of synovium-derived mesenchymal stem cells with collagen gel. Yokoyama, Akiko; Sekiya, Ichiro; Miyazaki, Kyosuke; Ichinose, Shizuko; Hata, Yuiro; Muneta, Takeshi // Cell & Tissue Research;Nov2005, Vol. 322 Issue 2, p289 

    Graft implantation is one of the more popular procedures for repairing cartilage defects; however, sacrifices of the donor site have been an issue. Mesenchymal stem cells (MSCs) are a fascinating source for regenerative medicine because they can be harvested in a less invasive manner and are...

  • Biochemical and Structural Characterization of Neocartilage Formed by Mesenchymal Stem Cells in Alginate Hydrogels. Olderøy, Magnus Ø.; Lilledahl, Magnus B.; Beckwith, Marianne Sandvold; Melvik, Jan Egil; Reinholt, Finn; Sikorski, Pawel; Brinchmann, Jan E. // PLoS ONE;Mar2014, Vol. 9 Issue 3, p1 

    A popular approach to make neocartilage in vitro is to immobilize cells with chondrogenic potential in hydrogels. However, functional cartilage cannot be obtained by control of cells only, as function of cartilage is largely dictated by architecture of extracellular matrix (ECM). Therefore,...

  • Matrix Development in Self-Assembly of Articular Cartilage. Ofek, Gidon; Revell, Christopher M.; Hu, Jerry C.; Allison, David D.; Grande-Allen, K. Jane; Athanasiou, Kyriacos A. // PLoS ONE;2008, Vol. 3 Issue 7, p1 

    Background: Articular cartilage is a highly functional tissue which covers the ends of long bones and serves to ensure proper joint movement. A tissue engineering approach that recapitulates the developmental characteristics of articular cartilage can be used to examine the maturation and...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics