Influence of 3-hour abstinence on sperm DNA fragmentation and the maturity of sperm chromatin

Lakpour, N.; Amirjannati, N.; Shani, S.; Akhondi, M.; Fathi, Z.; Bolourzadeh, M.; Jahromi, N. Saadaei; Sadeghi, M.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p119
Academic Journal
Introduction: Repeated ejaculation was suggested that accompanies with decrease in sperm DNA fragmentation (SDF). Therefore, we conducted this study to evaluate efficacy of 3-hour abstinence in reducing SDF and sperm chromation maturity in fertile men. Materials and Methods: Thirty seven fertile men were asked to give up from ejaculation for a period of 3-7 days and then give one semen sample (raw 1), followed by a period of 3-hour abstinence (raw 3). Sperm processing was performed using swim up method for first (raw1) and second (raw3) ejaculate of each person to produce corresponding neat samples of N1 and N2, respectively. SDF and sperm chromatin immaturity (SCI) were assayed by SDFA and SCMA kit for raw (raw1 and raw2) and neat (N1 and N3) samples. Results: SDF in the raw 1 and N1 groups showed no signiflcant differenee in comparison with raw 3 and N3 groups while it showed a signiflcant decrease in raw 1 and raw 3 groups compared to N1 and N3 groups (24.22±L65 vs. 16.83±1.66 and 24.72±1.71 vs. 17.78±1.59). On the other hands, SCI increased signiflcantly in Raw 3 group compared to Raw 1 (29.2±2.4 vs. 24.5±1.7) while it did not differ statistically between N1 and Raw 1 groups, N3 and Raw 3 groups as well as between N1 and N3 groups. Conclusion: We conclude that 3-hour abstinence cannot decrease sperm DNA fragmentation in fertile men while it cause a slight increase in shedding immature sperm in ejaculate which can partially be excluded using swim up sperm washing method.


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