TITLE

Association of plasminogen activator inhibitor-1 (PAI-1) polymorphisms with recurrent pregnancy loss

AUTHOR(S)
Shakarami, F.; Akbari, M.; Karizi, S. Zare
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p15
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Introduction: Recurrent pregnancy loss (RPL) refers to the occurrence of two or more consecutive losses of clinically recognized pregnancies prior to the 20th week of gestation. Several factors play a role in recurrent pregnancy loss (RPL) including thrombophilic conditions which can be influenced by gene polymorphisms. Plasminogen activator inhibitor-1 (PAI-1) is involved in the fibrinolytic process, and several studies have reported the association between this gene polymorphism and recurrent pregnancy loss (RPL). Materials and Methods: We studied the PAI-1 4G/5G polymorphism in women with recurrent abortion. 100 patients with recurrent abortions (at least two) as cases and 100 healthy female with two or more normal term deliveries and without a history of abortion selected as controls. Total genomic DNA was isolated from blood leukocytes. The status of the PAI-1 4G/5G polymorphism was determined by PCR-RFLP. Amplifying the corresponding region of genomic DNA followed by treatment with restriction enzyme Bse RI. Results: Homozygosity for PAI-1 4G polymorphism was seen in 17 cases (17%), in contrast to 5 persons in the control group (5%), (p=0.006) and patients with homozygote 4G mutation were significantly more prone to RPL in contrast to others (OR: 4.63, % 95 CI: 1.55-13.84). Conclusion: Considering these results, because 4G/4G polymorphism for PAI-1 gene could be a thrombophilic mutation leading to abortion, Analysis of this mutation and other suspected factors such as MTHFR and FV Leiden is recommended in patients with RPL.
ACCESSION #
96841512

 

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