TITLE

Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL

AUTHOR(S)
Hirata, Kunio; Shinzawa-Itoh, Kyoko; Yano, Naomine; Takemura, Shuhei; Kato, Koji; Hatanaka, Miki; Muramoto, Kazumasa; Kawahara, Takako; Tsukihara, Tomitake; Yamashita, Eiki; Tono, Kensuke; Ueno, Go; Hikima, Takaaki; Murakami, Hironori; Inubushi, Yuichi; Yabashi, Makina; Ishikawa, Tetsuya; Yamamoto, Masaki; Ogura, Takashi; Sugimoto, Hiroshi
PUB. DATE
July 2014
SOURCE
Nature Methods;Jul2014, Vol. 11 Issue 7, p734
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We report a method of femtosecond crystallography for solving radiation damage-free crystal structures of large proteins at sub-angstrom spatial resolution, using a large single crystal and the femtosecond pulses of an X-ray free-electron laser (XFEL). We demonstrated the performance of the method by determining a 1.9-Ã… radiation damage-free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.
ACCESSION #
96838160

 

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