Identification of Rhizobacteria from Ludwigia octovalvis Grown in Arsenic

Harmin Sulistiyaning Titah; Siti Rozaimah Sheikh Abdullah; Idris Mushrifah; Nurina Anuar; Hassan Basri; Muhammad Mukhlisin
June 2014
Australian Journal of Basic & Applied Sciences;2014 Special, Vol. 8 Issue 8, p134
Academic Journal
Rhizobacteria were isolated from the roots of Ludwigia octovalvis (Jacq.) Raven plants which grown in different places contaminated arsenic. Identification was conducted using two methods (Biolog GEN III and Vitek2 Compact) which were based on biochemical tests. All the identified rhizobacteria were Gram-positive bacteria: Arthrobacter globiformis, Bacillus megaterium, Bacillus cereus, Bacillus pumilus, Staphylococcus lentus, and also Gram-negative: Enterobacter asburiae, Sphingomonas paucimobilis, Pantoea spp, Rhizobium rhizogenes, Rhizobium radiobacter. The X code was identified as Rhizobium genus using Biolog GEN III method, this rhizobacteria was identified as R. radiobacter using Vitek2 Compact system. It indicates that two methods of identification could complement each other. Eleven rhizobacteria can be identified using Vitek2 Compact system, and another four rhizobacteria can be identified using Biolog GEN III method. In conclusion, Vitek2 Compact System method can identify more species of rhizobacteria than Biolog GEN III method.


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