TITLE

Hyperglycemia enhances function and differentiation of adult rat cardiac fibroblasts1

AUTHOR(S)
Shamhart, Patricia E.; Luther, Daniel J.; Adapala, Ravi K.; Bryant, Jennifer E.; Petersen, Kyle A.; Meszaros, J. Gary; Thodeti, Charles K
PUB. DATE
July 2014
SOURCE
Canadian Journal of Physiology & Pharmacology;Jul2014, Vol. 92 Issue 7, p598
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Diabetes is an independent risk factor for cardiovascular disease that can eventually cause cardiomyopathy and heart failure. Cardiac fibroblasts (CF) are the critical mediators of physiological and pathological cardiac remodeling; however, the effects of hyperglycemia on cardiac fibroblast function and differentiation is not well known. Here, we performed a comprehensive investigation on the effects of hyperglycemia on cardiac fibroblasts and show that hyperglycemia enhances cardiac fibroblast function and differentiation. We found that high glucose treatment increased collagen I, III, and VI gene expression in rat adult cardiac fibroblasts. Interestingly, hyperglycemia increased CF migration and proliferation that is augmented by collagen I and III. Surprisingly, we found that short term hyperglycemia transiently inhibited ERK1/2 activation but increased AKT phosphorylation. Finally, high glucose treatment increased spontaneous differentiation of cardiac fibroblasts to myofibroblasts with increasing passage compared with low glucose. Taken together, these findings suggest that hyperglycemia induces cardiac fibrosis by modulating collagen expression, migration, proliferation, and differentiation of cardiac fibroblasts.
ACCESSION #
96729330

 

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics