TITLE

Brucella infections in high-risk population and in patients hospitalized for fever: A serological study at Kolar, Karnataka

AUTHOR(S)
Aniyappanavar, Dhanalaxmi; Prasad, Subba Rama; Tanveer, Khaji Mohammed; Rao, Srinivasa
PUB. DATE
September 2013
SOURCE
Annals of Tropical Medicine & Public Health;Sep-Oct2014, Vol. 6 Issue 5, p549
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Brucellosis, one of the world's major zoonoses, is endemic in many parts of India. It is an occupational hazard for veterinary employees, butchers, dairy personnel, and laboratory workers. There is no information available on human brucellosis from Kolar region of southern India. Methods and Materials: Serum samples from 154 adults, at high risk for brucellosis and residing in and around Kolar, Karnataka, India, were screened for antibodies to brucella by the Rose Bengal plate agglutination test and immunoglobulin (Ig)G Enzyme-linked immunosorbent assay (ELISA). Sera detected to be positive by these screening tests were further tested by standard tube agglutination test (SAT), SAT with 2 Mercaptoethanol, and IgM ELISA. In addition, serum samples from 100 hospitalized patients with prolonged fever were also subjected to the above tests to detect acute brucella infections among patients. Results: Among the 154 individuals at high-risk screened, brucella antibodies were detected in 15 (9.74%) of the subjects. The seropositivity rate was 30.76% among veterinarians, 14.28 among cattle businessmen, 9.67% among butchers, and 3.79% among animal owners. Seropositivity was associated with drawing blood and exposure to animals during parturition. Symptoms such as myalgia, low back ache, and joint pains were significantly more among seropositives. When the results of all the tests were taken together, 33.3% seropositives could be classified as past brucella infections and 66.6% as possible chronic brucella infections. Among the 100 hospitalized patients with prolonged fever acute brucellosis was diagnosed in one patient. Conclusion: Brucellosis is an important zoonosis in Kolar region. Among individuals at high risk many have serological profile of possible chronic brucellosis; such individuals may need treatment and follow-up.
ACCESSION #
96564594

 

Related Articles

  • Evaluation of ELISA and Brucellacapt tests for diagnosis of human Brucellosis. Peeridogaheh, Hadi; Golmohammadi, Mohammad Ghasem; Pourfarzi, Farhad // Iranian Journal of Microbiology;Mar2013, Vol. 5 Issue 1, p14 

    Background and Objectives: Brucellosis is one of the most common zoonotic diseases in Iran and human brucellosis is endemic in all parts of the country. Because of the difficulty in the diagnosis of brucellosis, particularly in endemic areas, the use of new and feasible diagnostic tests seem to...

  • Development and Comparative Evaluation of a Competitive ELISA with Rose Bengal Test and a Commercial Indirect ELISA for Serological Diagnosis of Brucellosis. Mythili, Tadepalli; Rajendra, Lingala; Bhavesh, Trangadia; Thiagarajan, Dorairajan; Srinivasan, Villuppanoor // Indian Journal of Microbiology;Oct2011, Vol. 51 Issue 4, p528 

    The development of a competitive ELISA for the detection of brucella-specific antibodies in bovines is described. Anti-brucella guinea pig serum was used as a source of competing antibodies. Lipo-polysaccharide purified from inactivated B. abortus S19 culture was used as antigen for the...

  • Comparison of the Diagnostic Value of the Standard Tube Agglutination Test and the ELISA IgG and IgM in Patients with Brucellosis. Ertek, Mustafa; Yazgi, Halil; Özkurt, Zülal; Ayyildiz, Ahmet; Parlak, Mehmet // Turkish Journal of Medical Sciences;2006, Vol. 36 Issue 3, p159 

    Background and Aims: Brucellosis is endemic in Turkey. Since it affects many organs and the symptoms are non-specific, the diagnosis by clinical findings is difficult and may be easily missed. Many serological tests have been used for the diagnosis of human brucellosis. This study compared the...

  • The Value of Serologic Tests for Diagnosis and Follow up of Patients having Brucellosis. Lucero, Nidia E.; Ayala, Sandra M.; Escobar, Gabriela I.; Jacob, Néstor R. // American Journal of Infectious Diseases;2007, Vol. 3 Issue 1, p27 

    Though diagnosis of human brucellosis is accurate when the causal agent is isolated, this procedure is not always successful and the most of patients are diagnosed on the basis of rising titres of antibodies in serum. The classical tests used for detection of antibodies to S-Brucella sp.,...

  • Serological investigation of bovine brucellosis in three cattle production systems in Yewa Division, south-western Nigeria. Cadmus, Simeon I. B.; Alabi, Peter I.; Adesokan, Hezekiah K.; Dale, Emma J.; Stack, Judy A. // Journal of the South African Veterinary Association;2013, Vol. 84 Issue 1, p1 

    Limited data are available on the risk factors responsible for the occurrence of brucellosis amongst different cattle production systems in Nigeria despite its significant impact on livestock production. Consequently, a cross-sectional study was conducted to determine the prevalence of bovine...

  • Validation of a simple universal IELISA for the diagnosis of human brucellosis. Ayala, S.; Hasan, D.; Celestino, C.; Escobar, G.; Zhao, D.; Lucero, N. // European Journal of Clinical Microbiology & Infectious Diseases;Jul2014, Vol. 33 Issue 7, p1239 

    The definitive diagnosis of brucellosis requires isolation of the agent, although negative isolation does not rule out the infection. In contrast, serological testing is more sensitive and, therefore, preferred in clinical practice. The majority of reported cases around the world were caused by...

  • Study of the Assimilation Rate of Immunoenzymatic Tests and Traditional Serological Methods in the Diagnosis of Human Brucellosis. Bashiri, Homayoon; Sayad, Babak; Madani, Seyed Hamid // Jundishapur Journal of Microbiology;Jun2013, Vol. 6 Issue 4, p1 

    Background: Brucellosis is a disease carried by animals that can be transmitted to humans. The signs and symptoms of brucellosis are nonspecific, blood tests and blood/tissue cultures are necessary for making the diagnosis of brucellosis. Testing for antibodies against the bacteria and isolating...

  • Comparison between ELISA and other serological tests for diagnosis of brucellosis in sheep in Ninevah Province. AI-Hankawe, O. K. H.; Rhaymah, M. S. // Iraqi Journal of Veterinary Sciences;2012 Supplement II, following p75 

    The study was conducted to compare between the ELISA test and other serological tests (Rose bengal, modified rose bengal, tube agglutination and 2-mercapto-ethanol tests), for diagnosis of Brucellosis in sheep, and to identify the prevalence of the disease. The study included 5723 heads...

  • Epidemiology of Brucellosis and Q Fever in Linked Human and Animal Populations in Northern Togo. Dean, Anna S.; Bonfoh, Bassirou; Kulo, Abalo E.; Boukaya, G. Aboudou; Amidou, Moussa; Hattendorf, Jan; Pilo, Paola; Schelling, Esther // PLoS ONE;Aug2013, Vol. 8 Issue 8, p1 

    Background: Although brucellosis (Brucella spp.) and Q Fever (Coxiella burnetii) are zoonoses of global importance, very little high quality data are available from West Africa. Methods/Principal Findings: A serosurvey was conducted in Togo’s main livestock-raising zone in 2011 in 25...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics