Induction of human plasmablasts during infection with antibiotic-resistant nosocomial bacteria

Band, Victor I.; Ibegbu, Chris; Kaur, Surinder Pal; Cagle, Stephanie M.; Trible, Ronald; Jones, Crystal L.; Wang, Yun F.; Kraft, Colleen S.; Ray, Susan M.; Wrammert, Jens; Weiss, David S.
July 2014
Journal of Antimicrobial Chemotherapy (JAC);Jul2014, Vol. 69 Issue 7, p1830
Academic Journal
Objectives Nosocomial pathogens such as Acinetobacter baumannii are a growing public health threat, due in part to their increasing resistance to antibiotics. Since some strains are resistant to all available antibiotics, novel therapies are urgently needed. Plasmablasts are short-lived B cells found in the blood that can be collected and harnessed to produce therapeutic antibodies. We set out to determine whether plasmablasts are induced during infection with A. baumannii and other nosocomial pathogens. Methods We obtained blood samples from patients infected with antibiotic-resistant nosocomial pathogens, and analysed their plasmablast response by flow cytometry. Results We observed a strong induction of plasmablasts in patients with antibiotic-resistant A. baumannii infection. Furthermore, plasmablasts were also induced in response to other drug-resistant nosocomial pathogens. Conclusions These data suggest that plasmablasts may be broadly harnessed to develop therapeutic antibodies to combat otherwise untreatable antibiotic-resistant infections.


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