A high throughput RNAi screen reveals determinants of HIV-1 activity in host kinases

Wei-Min Jiang; Xin-Yun Zhang; Yun-Zhi Zhang; Li Liu; Hong-Zhou Lu
May 2014
International Journal of Clinical & Experimental Pathology;2014, Vol. 7 Issue 5, p2229
Academic Journal
No abstract available.


Related Articles

  • Insights to transcriptional networks by using high throughput RNAi strategies. Mattila, Jaakko; Puig, Oscar // Briefings in Functional Genomics;Jan2010, Vol. 9 Issue 1, p43 

    RNA interference (RNAi) is a powerful method to unravel the role of a given gene in eukaryotic cells. The development of high throughput assay platforms such as fluorescence plate readers and high throughput microscopy has allowed the design of genome wide RNAi screens to systemically discern...

  • A parallel genome-wide RNAi screening strategy to identify host proteins important for entry of Marburg virus and H5N1 influenza virus. Han Cheng; Koning, Katie; O'Hearn, Aileen; Minxiu Wang; Rumschlag-Booms, Emily; Varhegyi, Elizabeth; Lijun Rong // Virology Journal;11/24/2015, Vol. 12 Issue 1, p1 

    Background: Genome-wide RNAi screening has been widely used to identify host proteins involved in replication and infection of different viruses, and numerous host factors are implicated in the replication cycles of these viruses, demonstrating the power of this approach. However, discrepancies...

  • Cellular Assays for High-Throughput Screening for Modulators of Trk Receptor Tyrosine Kinases. Jun Wang; Hancock, Michael K.; Dudek, Jeanne M.; Kun Bi // Current Chemical Genomics;2009, Vol. 2, p27 

    Trk receptor tyrosine kinases are required for signal transduction initiated by neurotrophins leading to cell proliferation, differentiation, survival and death. Alterations in Trk kinase activity have been linked to various diseases. To address the need for cell-based assays for screening and...

  • Integrated Functional, Gene Expression and Genomic Analysis for the Identification of Cancer Targets. Iorns, Elizabeth; Lord, Christopher J.; Grigoriadis, Anita; McDonald, Sarah; Fenwick, Kerry; MacKay, Alan; Mein, Charles A.; Natrajan, Rachael; Savage, Kay; Tamber, Narinder; Reis-Filho, Jorge S.; Turner, Nicholas C.; Ashworth, Alan // PLoS ONE;2009, Vol. 4 Issue 4, p1 

    The majority of new drug approvals for cancer are based on existing therapeutic targets. One approach to the identification of novel targets is to perform high-throughput RNA interference (RNAi) cellular viability screens. We describe a novel approach combining RNAi screening in multiple cell...

  • Stimulation of Host Immune Defenses by a Small Molecule Protects C. elegans from Bacterial Infection. Pukkila-Worley, Read; Feinbaum, Rhonda; Kirienko, Natalia V.; Larkins-Ford, Jonah; Conery, Annie L.; Ausubel, Frederick M. // PLoS Genetics;Jun2012, Vol. 8 Issue 6, Special section p1 

    The nematode Caenorhabditis elegans offers currently untapped potential for carrying out high-throughput, live-animal screens of low molecular weight compound libraries to identify molecules that target a variety of cellular processes. We previously used a bacterial infection assay in C. elegans...

  • A novel specific edge effect correction method for RNA interference screenings. Carralot, Jean-Philippe; Ogier, Arnaud; Boese, Annette; Genovesio, Auguste; Brodin, Priscille; Sommer, Peter; Dorval, Thierry // Bioinformatics;Jan2012, Vol. 28 Issue 2, p261 

    Motivation: High-throughput screening (HTS) is an important method in drug discovery in which the activities of a large number of candidate chemicals or genetic materials are rapidly evaluated. Data are usually obtained by measurements on samples in microwell plates and are often subjected to...

  • Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays. Kulkarni, Meghana M.; Booker, Matthew; Silver, Serena J.; Friedman, Adam; Pengyu Hong; Perrimon, Norbert; Mathey-Prevot, Bernard // Nature Methods;Oct2006, Vol. 3 Issue 10, p1 

    To evaluate the specificity of long dsRNAs used in high-throughput RNA interference (RNAi) screens performed at the Drosophila RNAi Screening Center (DRSC), we performed a global analysis of their activity in 30 genome-wide screens completed at our facility. Notably, our analysis predicts that...

  • Domain-Based Biosensor Assay to Screen for Epidermal Growth Factor Receptor Modulators in Live Cells. Antczak, Christophe; Bermingham, Alun; Calder, Paul; Malkov, Dmitry; Song, Keming; Fetter, John; Djaballah, Hakim // Assay & Drug Development Technologies;Feb2012, Vol. 10 Issue 1, p24 

    AbstractTraditional drug discovery efforts have resulted in the approval of a handful of receptor tyrosine kinase (RTK) inhibitors; however, their discovery relied solely on screening recombinant kinases, often with poor cellular activity outcome. The ability to screen RTKs in their natural...

  • RNAi Screen Reveals an Abl Kinase-Dependent Host Cell Pathway Involved in Pseudomonas aeruginosa Internalization. Pielage, Julia F.; Powell, Kimberly R.; Kalman, Daniel; Engel, Joanne N. // PLoS Pathogens;Mar2008, Vol. 4 Issue 3, p1 

    Internalization of the pathogenic bacterium Pseudomonas aeruginosa by non-phagocytic cells is promoted by rearrangements of the actin cytoskeleton, but the host pathways usurped by this bacterium are not clearly understood. We used RNAi-mediated gene inactivation of ∼80 genes known to...

  • Antiviral Stratagems Against HIV-1 Using RNA Interference (RNAi) Technology. Vlachakis, Dimitrios; Tsiliki, Georgia; Pavlopoulou, Athanasia; Roubelakis, Maria G.; Champeris Tsaniras, Spyridon; Kossida, Sophia // Evolutionary Bioinformatics;2013, Issue 9, p203 

    The versatility of human immunodeficiency virus (HIV)-1 and its evolutionary potential to elude antiretroviral agents by mutating may be its most invincible weapon. Viruses, including HIV, in order to adapt and survive in their environment evolve at extremely fast rates. Given that conventional...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics