A transgenic approach to controlling wheat seed dormancy level by using Triticeae DOG1-like genes

Ashikawa, Ikuo; Mori, Masahiko; Nakamura, Shingo; Abe, Fumitaka
August 2014
Transgenic Research;Aug2014, Vol. 23 Issue 4, p621
Academic Journal
Seed dormancy is an important agronomic trait: low levels can cause premature germination, while too much can inhibit uniform germination. As an approach to controlling the seed dormancy level in crops, we used Triticeae DOG1-like genes as transgenes. DOG1 is an Arabidopsis gene that underlies natural variation in seed dormancy. We previously showed that although their sequence similarities to DOG1 were low, some cereal DOG1-like genes enhanced seed dormancy in Arabidopsis. Here, we introduced two DOG1-like genes, TaDOG1L4 from wheat and HvDOG1L1 from barley, individually into the wheat cultivar Fielder. Their overexpression under the control of a maize ubiquitin promoter enhanced the seed dormancy level while leaving other traits unchanged. TaDOG1L4 was more effective than HvDOG1L1, which accords with the previously revealed difference in the effectiveness of these two genes in Arabidopsis seed dormancy. Knockdown of endogenous TaDOG1L4 in Fielder using double-strand RNA interference decreased the seed dormancy level by several tens of percent. This result indicates that some degree of seed dormancy inherent in wheat is imparted by DOG1-like genes.


Related Articles

  • TaABI5, a wheat homolog of Arabidopsis thaliana ABA insensitive 5, controls seed germination. Utsugi, Shigeko; Ashikawa, Ikuo; Nakamura, Shingo; Shibasaka, Mineo // Journal of Plant Research;Mar2020, Vol. 133 Issue 2, p245 

    Abscisic acid (ABA) response element (ABRE)-binding factors (ABFs) are basic region/leucine zipper motif (bZIP) transcription factors that regulate the expression of ABA-induced genes containing ABRE in their promoters. The amino acid sequence of the wheat bZIP protein, TaABI5, showed high...

  • Arabidopsis IQM4, a Novel Calmodulin-Binding Protein, Is Involved With Seed Dormancy and Germination in Arabidopsis. Yu Ping Zhou; Jing Hui Wu; Wen Hui Xiao; Wei Chen; Qiong Hua Chen; Tian Fan; Chu Ping Xie; Chang-En Tian // Frontiers in Plant Science;6/5/2018, p1 

    Seed dormancy and germination are regulated by complex mechanisms controlled by diverse hormones and environmental cues. Abscisic acid (ABA) promotes seed dormancy and inhibits seed germination and post-germination growth. Calmodulin (CaM) signals are involved with the inhibition of ABA during...

  • Dormant and after-Ripened Arabidopsis thaliana Seeds are Distinguished by Early Transcriptional Differences in the Imbibed State. Dekkers, Bas J. W.; Pearce, Simon P.; van Bolderen-Veldkamp, R. P. M.; Holdsworth, Michael J.; Bentsink, Leónie // Frontiers in Plant Science;8/30/2016, p1 

    Seed dormancy is a genetically controlled block preventing the germination of imbibed seeds in favorable conditions. It requires a period of dry storage (after-ripening) or certain environmental conditions to be overcome. Dormancy is an important seed trait, which is under selective pressure, to...

  • Genome-wide association study and quantitative trait loci mapping of seed dormancy in common wheat (Triticum aestivum L.). Cao, Hong; Chen, Fengying; Liu, Jindong; Zuo, Jinghong; Liu, Yongxiu; Lin, Chih-Ta // Planta;Jul2019, Vol. 250 Issue 1, p187 

    Main conclusion: Totally, 23 and 26 loci for the first count germination ratio and the final germination ratio were detected by quantitative trait loci (QTL) mapping and association mapping, respectively, which could be used to facilitate wheat pre-harvest sprouting breeding. Weak dormancy can...

  • A role for an alternative splice variant of PIF6 in the control of Arabidopsis primary seed dormancy. Penfield, Steven; Josse, Eve-Marie; Halliday, Karen J. // Plant Molecular Biology;May2010, Vol. 73 Issue 1/2, p89 

    Phytochrome interacting factor (PIF) transcription factors have been shown to be important in the regulation of seed dormancy and germination by environmental cues. Many PIF-family transcription factors are expressed in seeds but only PIF1 and SPATULA (SPT) have been tested for a role in...

  • Tetrazolium test adjustment for wheat seeds. de Carvalho, Tereza Cristina; Krzyzanowski, Francisco Carlos; de Castro Ohlson, Osvaldo; Panobianco, Maristela // Journal of Seed Science;set2013, Vol. 35 Issue 3, p361 

    The assessment of the germination test in wheat seeds varies from 4 to 15 days, because the species normally presents dormancy in freshly harvested seeds. The tetrazolium test can characterize seed viability in less than 24 hours including lots with dormancy seeds. The objective of this study...

  • Arabidopsis thaliana SEED DORMANCY 4-LIKE regulates dormancy and germination by mediating the gibberellin pathway. Cao, Hong; Han, Yi; Li, Jingyi; Ding, Meng; Li, Yu; Li, Xiaoying; Chen, Fengying; Soppe, Wim Jj; Liu, Yongxiu // Journal of Experimental Botany;1/23/2020, Vol. 71 Issue 3, p919 

    The molecular mechanisms underlying seed dormancy and germination are not fully understood. Here, we show that Arabidopsis thaliana SEED DORMANCY 4-LIKE (AtSdr4L) is a novel specific regulator of dormancy and germination. AtSdr4L encodes a protein with an unknown biochemical function that is...

  • Nitric oxide implication in the control of seed dormancy and germination. Arc, Erwann; Galland, Marc; Godin, Béatrice; Cueff, Gwendal; Rajjou, Loïc // Frontiers in Plant Science;Sep2013, Vol. 4, p1 

    Germination ability is regulated by a combination of environmental and endogenous signals with both synergistic and antagonistic effects. Nitric oxide (NO) is a potent dormancy-releasing agent in many species, including Arabidopsis, and has been suggested to behave as an endogenous regulator of...

  • H2O2 mediates the regulation of ABA catabolism and GA biosynthesis in Arabidopsis seed dormancy and germination. Yinggao Liu; Nenghui Ye; Rui Liu; Moxian Chen; Jianhua Zhang // Journal of Experimental Botany;Oct2010, Vol. 61 Issue 11, p2979 

    H2O2 is known as a signal molecule in plant cells, but its role in the regulation of aqbscisic acid (ABA) and gibberellic acid (GA) metabolism and hormonal balance is not yet clear. In this study it was found that H2O2 affected the regulation of ABA catabolism and GA biosynthesis during seed...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics