Effect of long-term treatment of morphine on enzymes, oxidative stress indices and antioxidant status in male rat liver

Samarghandian, Saeed; Afshari, Reza; Farkhondeh, Tahereh
June 2014
International Journal of Clinical & Experimental Medicine;2014, Vol. 7 Issue 5, p1449
Academic Journal
In this study, biochemical changes due to long term usage of morphine in rat's liver were assessed. Twenty male Wistar rats (180-220 g) were included and divided into two groups. Normal saline was given intraperitoneally in the control group (n = 10). Morphine group (n = 10) received morphine intraperitoneally at a dose of 4, 8, and 10 mg/kg/day in the first, second and the third ten days of the study, respectively. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and liver malondialdehyde (MDA) level as well as activities of superoxide dismutase (SOD), glutathione-s-transfrase (GST) and catalase (CAT) were measured. Serum levels of AST, ALT and LDH were significantly higher in the morphine group compared with the control group. The mean MDA level of liver was significantly higher in the morphine group compared with the control group (P < 0.05). The activities of SOD, GST and CAT were significantly lower in the morphine group compared with the control group (P < 0.01). Our findings pointed out the risk of hepatic damage due to long term usage of morphine via disturbance oxidant-antioxidant balance. Although morphine is showed to be effective in pain treatment, their toxic effects should be kept in mind during the chronic usage.


Related Articles

  • Deep hypothermia impact on acid-base parameters and liver antioxidant status in an in vivo rat model. Alva, Norma; Carbonell, Teresa; Palomeque, Jesús // Canadian Journal of Physiology & Pharmacology;Jun2009, Vol. 87 Issue 6, p471 

    Although clinical hypothermia is used for reducing postischemic damage, injurious effects have also been reported. To determine whether hypoxia and oxidative stress are induced by systemic deep hypothermia, we used an in vivo rat model keeping the arterial Pco2...

  • Protective effects of roasted coffee drink on liver function of cirrhotic rats. Neto, Youssef A. H.; Rocha, Bruno A.; Pedroso, Rodrigo N.; Neto, Marthus M. F .; Paula, Fernanda B. A.; Duarte, Stella M. S. // Journal of Natural Pharmaceuticals;Dec2010, Vol. 1 Issue 1, p19 

    The role of oxidative stress as a major cause of tissue injury has been suggested and it has been demonstrated that changes in the oxidant and antioxidant mechanisms contribute largely to hepatic necrosis in these situations. However, the increased intake of foods rich in antioxidants could help...

  • Oxidative Stress in the Prefrontal Cortex as a Factor Responsible for Morphine Administration-Related Depression in Rats. Famitafreshi, H.; Karimian, M. // Neurophysiology;Jul2019, Vol. 51 Issue 4, p253 

    We examined the possible role of oxidative stress in the prefrontal cortex for the development of depression induced by course morphine administration. Thirty-two male albino rats were divided into four groups: control intact (CI), control saline-treated (CS), morphine (5 mg/kg) treated (M), and...

  • Oxidative stress in rat liver during acute cadmium and ethanol intoxication. Radosavljević, Tatjana; Mladenović, Dušan; Ninković, Milica; Vučević, Danijela; Boričić, Ivan; Ješićukićević, Rada; Šljivančanin, Tamara; Lopičić, Srdjan; Todorović, Vera // Journal of the Serbian Chemical Society;Feb2012, Vol. 77 Issue 2, p159 

    The aim of this study was to investigate the effects of binge drinking on the prooxidant/antioxidant system in rat liver in acute cadmium (Cd) intoxication. Male Wistar rats were used in the experiments. They were divided into the following groups: 1. control, 2. ethanol-treated group, in five...

  • The effects of different periods of renal ischemia/reperfusion injury on rats' liver function, cytokine levels and antioxidant status. Kadkhodaee, M.; Golab, F.; Zahmatkesh, M.; Ghaznavi, R.; Hedayati, M.; Arab, H. A.; Soleimani, M. // Tehran University Medical Journal;Oct2009, Vol. 67 Issue 7, p464 

    Background: The effect of ischemia/reperfusion (I/R) injury on kidney has been under investigation for many years. But the changes in liver function and oxidative stress status in renal I/R injury is not well known. Recent studies suggest a crosstalk between liver and kidneys. The aim of the...

  • Protective effects of Gingko biloba on thioacetamide-induced fulminant hepatic failure in rats. Harputluoglu, M. M. M.; Demirel, U.; Ciralik, H.; Temel, I.; Firat, S.; Ara, C.; Aladag, M.; Karincaoglu, M.; Hilmioglu, F. // Human & Experimental Toxicology;Dec2006, Vol. 25 Issue 12, p705 

    Gingko biloba (GB) has antioxidant and platelet-activating factor (PAF) antagonistic effects. We investigated the protective effects of GB on thioacetamide (TAA)-induced fulminant hepatic failure in rats. Fulminant hepatic failure was induced in treatment groups by three intraperitoneal (ip)...

  • Effects of diallyl tetrasulfide on cadmiuminduced oxidative damage in the liver of rats. Murugavel, P.; Pari, L. // Human & Experimental Toxicology;Jun2007, Vol. 26 Issue 6, p527 

    The protective efficacy of diallyl tetrasulfide (DTS) from garlic on liver injury induced by cadmium (Cd) was investigated. In this study, Cd (3 mg/kg body weight) was administered subcutaneously for 3 weeks to induce toxicity. DTS was administered orally (10, 20 and 40 mg/kg body weight) for 3...

  • Apocynin administration prevents the changes induced by a fructose-rich diet on rat liver metabolism and the antioxidant system. CASTRO, María Cecilia; FRANCINI, Flavio; SCHINELLA, Guillermo; CALDIZ, Claudia Inés; ZUBIRÍA, María Guillermina; GAGLIARDINO, Juan José; MASSA, María Laura // Clinical Science;2012, Vol. 123 Issue 12, p681 

    In the present study, we investigated the role of NADPH oxidase in F (fructose)-rich-dietinduced hepatic OS (oxidative stress) and metabolic changes, and their prevention by apocynin co-administration. Wistar rats were fed for 21 days on (i) a control diet, (ii) a control diet plus 10% F in the...

  • A COMPARATIVE STUDY OF THERESTORATIVE EFFECTS OF OCIMUM GRATISSIMUM AND OCIMUM CANUM ON ALCOHOL INDUCED HEPATOTOXICITY IN ALBINO RATS. George, S.; Chaturvedi, P.; Moseki, B. // African Journal of Food, Agriculture, Nutrition & Development;Nov2011, Vol. 11, p5614 

    Many species of Ocimum possess anti-inflammatory, antioxidant and anti-microbial properties and the plants are widely used in Ayurveda. The oxidative stress induced by ethanol consumption had been implicated the changes in the structure and functions of liver cells especially the membrane...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics