Nucleic Acid Amplification Tests for the Diagnosis of Pneumonia

Murdoch, David R.
May 2003
Clinical Infectious Diseases;5/1/2003, Vol. 36 Issue 9, p1162
Academic Journal
Molecular diagnostic techniques, such as polymerase chain reaction (PCR), are promising tools for the rapid etiological diagnosis of pneumonia. PCR offers potential advantages over conventional tests for the detection of Mycoplasma pneumoniae, Legionella species, and Chlamydia pneumoniae. For pneumococcal pneumonia in adults, PCR adds little to existing diagnostic tests and is unable to distinguish pneumococcal colonization from infection when testing respiratory samples. Although PCR is probably more sensitive than are conventional microscopy-based methods for diagnosing Pneumocystis carinii pneumonia, the specificity is uncertain, because P. carinii can occasionally be detected in the absence of clinical symptoms. PCR is useful for the diagnosis of viral pneumonia in immunocompromised patients. Further work is required to better characterize the role of PCR versus the role of other tests for diagnosing pneumonia and to develop standard PCR assays that can be readily adopted by routine diagnostic laboratories.


Related Articles

  • Low-Cost 3D Printers Enable High-Quality and Automated Sample Preparation and Molecular Detection. Chan, Kamfai; Coen, Mauricio; Hardick, Justin; Gaydos, Charlotte A.; Wong, Kah-Yat; Smith, Clayton; Wilson, Scott A.; Vayugundla, Siva Praneeth; Wong, Season // PLoS ONE;6/30/2016, Vol. 11 Issue 6, p1 

    Most molecular diagnostic assays require upfront sample preparation steps to isolate the target’s nucleic acids, followed by its amplification and detection using various nucleic acid amplification techniques. Because molecular diagnostic methods are generally rather difficult to perform...

  • The PCR assay in the preclinical safety evaluation of nucleic acid medicines. Haworth, R.; Pilling, A.M. // Human & Experimental Toxicology;May2000, Vol. 19 Issue 5, p267 

    The polymerase chain reaction (PCR) is a highly efficient gene amplification procedure which is increasingly being applied to the safety assessment of nucleic acid (NA) medicines such as gene therapies and DNA vaccines. Although clinical experience is limited, a number of potential safety issues...

  • Chapter 7: Nucleic Acid Amplification. Buckingham, Lela; Flaws, Maribeth L. // Molecular Diagnostics: Fundamentals, Methods & Clinical Applicat;2007, p121 

    The article discusses the assays for amplifying nucleic acids which includes polymerase chain reaction, branched DNA amplification, ligase chain reaction. It also describes the detection of amplicons for the amplification methods. Comparison between target amplification and signal amplification...

  • Critical points of DNA quantification by real-time PCR -- effects of DNA extraction method and sample matrix on quantification of genetically modified organisms. Cankar, Katarina; Štebih, Dejan; Dreo, Tanja; Žel, Jana; Gruden, Kristina // BMC Biotechnology;2006, Vol. 6, p37 

    Background: Real-time PCR is the technique of choice for nucleic acid quantification. In the field of detection of genetically modified organisms (GMOs) quantification of biotech products may be required to fulfil legislative requirements. However, successful quantification depends crucially on...

  • chipPCR: an R package to pre-process raw data of amplification curves. Rödiger, Stefan; Burdukiewicz, Michał; Schierack, Peter // Bioinformatics;9/1/2015, Vol. 31 Issue 17, p2900 

    Motivation: Both the quantitative real-time polymerase chain reaction (qPCR) and quantitative isothermal amplification (qIA) are standard methods for nucleic acid quantification. Numerous real-time read-out technologies have been developed. Despite the continuous interest in amplification- based...

  • Polymerase reaction without primers throughout for the reconstruction of full-length cDNA from products of rapid amplification of cDNA ends (RACE). Sunohara, Mitsuhiro; Kawakami, Masanori; Kage, Hidenori; Watanabe, Kousuke; Emoto, Noriko; Nagase, Takahide; Ohishi, Nobuya; Takai, Daiya // Biotechnology Letters;Jul2011, Vol. 33 Issue 7, p1301 

    Rapid amplification of cDNA ends (RACE) has widely been used to determine both ends of the cDNA from its partial sequence. Conventionally, 5′- and 3′-RACE products were ligated at a restriction site in the overlap region to reconstruct the full-length cDNA; however, reconstruction is...

  • Successful use of saliva without DNA extraction for detection of macrolide-resistant Mycoplasma pneumoniae DNA in children using LNA probe-based real-time PCR. Komatsu, Haruki; Tsunoda, Tomoyuki; Inui, Ayano; Sogo, Tsuyoshi; Fujisawa, Tomoo; Imura, Motoki; Tateno, Akihiko // Journal of Infection & Chemotherapy (Springer Science & Business;Dec2013, Vol. 19 Issue 6, p1087 

    It is not known that saliva is useful to diagnose Mycoplasma pneumoniae ( M. pneumoniae) infection by PCR. We evaluated prospectively whether crude saliva samples without the DNA extraction process were useful for the detection of M. pneumoniae DNA in a locked nucleic acid (LNA) probe-based...

  • Comparison of reverse transcription loop-mediated isothermal amplification, conventional PCR and real-time PCR assays for Japanese encephalitis virus. Zhiyong Chen; Yuxue Liao; Xuemei Ke; Jie Zhou; Yixiong Chen; LuLu Gao; Qing Chen; Shouyi Yu // Molecular Biology Reports;Aug2011, Vol. 38 Issue 6, p4063 

    We developed and evaluated a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting Japanese encephalitis virus (JEV). The sensitivity of the JEV RT-LAMP assay was in concordance with that of real-time RT-PCR and 10-fold more sensitive than that of...

  • Rapid Salt-Extraction of Genomic DNA from Formalin-Fixed Toad and Frog Tissues for PCR-Based Analyses. Wu-Yi Liu; Ke-Jun Zhang // Asian Journal of Animal & Veterinary Advances;Sep2011, Vol. 6 Issue 9, p958 

    The technical procedures for extraction of DNA from formalin-fixed tissues include many steps such as chemical treatment, enzymatic digestion, phenol-chloroform purification and alcohol precipitation. Formalin-fixed specimens used in molecular cell and DNA studies have shown shortcomings with...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics