Sustained Hypoxic Pulmonary Vasoconstriction in the Isolated Perfused Rat Lung: Effect of α1-adrenergic Receptor Agonist

Ketabchi, Farzaneh; Karimi, Zinab; Moosavi, Seyed Mostafa S.
May 2014
Iranian Journal of Medical Sciences;May2014, Vol. 39 Issue 3, p275
Academic Journal
Background: Alveolar hypoxia induces monophasic pulmonary vasoconstriction in vivo, biphasic vasoconstriction in the isolated pulmonary artery, and controversial responses in the isolated perfused lung. Pulmonary vascular responses to sustained alveolar hypoxia have not been addressed in the isolated perfused rat lung. In this study, we investigated the effect of sustained hypoxic ventilation on pulmonary artery pressure in the present of phenylephrine, an α1-receptor agonist, under the above condition. Methods: We performed this study in the isolated perfused rat lung. After preparation, the lungs were divided randomly into five groups of normoxic-normocapnia, hypoxic-normocapnia, phenylephrine pre- or post-treated hypoxic-normocapnia and phenylephrine pre-treated normoxic-normocapnia. Pulmonary hemodynamic, airway pressure and lung weight were measured during 60 min of the experiment for each group. Results: In the phenylephrine-pre-treated hypoxic-normocapnia group we observed a gradual increase in pulmonary artery pressure which approximated the results seen in the phenylephrine-pre-treated normoxic-normocapnia group. In contrast, in the phenylephrine-post-treated hypoxic-normcapnic group, pulmonary artery pressure did not change during the first 3 min of hypoxic-normocapnia. However at 1.5 min after administration of phenylephrine, this pressure began to increase sharply and continued until the end of the experiment. This response was biphasic (0-10 min: acute phase, 10-60 min: sustained phase) with significantly higher pulmonary artery pressure compared to the other groups. Conclusion: This study, for the first time, showed biphasic hypoxic pulmonary vasoconstriction in the isolated perfused rat lung with the sole administration of phenylephrine after but not before hypoxic gas ventilation. This finding suggested a facilitative role of alveolar hypoxia on pulmonary vasoconstriction induced by an α1-receptor agonist.


Related Articles

  • Endothelium/nitric oxide mechanism mediates vasorelaxation and counteracts vasoconstriction induced by low concentration of flavanols. Aggio, Annalisa; Grassi, Davide; Onori, Eugenia; D'alessandro, Antonio; Masedu, Francesco; Valenti, Marco; Ferri, Claudio // European Journal of Nutrition;Feb2013, Vol. 52 Issue 1, p263 

    Purpose: At relatively low concentrations, flavanols induce inconsistent effects on isolated arterial tone, sometimes explained as being due to a structure-activity relationship. The aim of our study was to investigate the effects of two flavanols at different doses on arterial functional state....

  • Reduction of Ethanol Consumption in Alcohol-Preferring Rats by Dual Expression Gene Transfer. Rivera-Meza, Mario; Quintanilla, María Elena; Tampier, Lutske // Alcohol & Alcoholism;Mar/Apr2012, Vol. 47 Issue 2, p102 

    Aims: To mimic, in an animal model of alcoholism, the protective phenotype against alcohol consumption observed in humans carrying a fast alcohol dehydrogenase (ADH1B*2) and an inactive aldehyde dehydrogenase (ALDH2*2). Methods: We developed a multiple expression cassette adenoviral vector...

  • Alterations in Lipid Levels of Mitochondrial Membranes Induced by Amyloid-β: A Protective Role of Melatonin. Rosales-Corral, Sergio A.; Lopez-Armas, Gabriela; Cruz-Ramos, Jose; Melnikov, Valery G.; Dun-Xian Tan; Manchester, Lucien C.; Munoz, Ruben; Reiter, Russel J. // International Journal of Alzheimer's Disease;2012, p1 

    Alzheimer pathogenesis involves mitochondrial dysfunction, which is closely related to amyloid-β (Aβ) generation, abnormal tau phosphorylation, oxidative stress, and apoptosis. Alterations in membranal components, including cholesterol and fatty acids, their characteristics, disposition,...

  • Effects of different extracts of kanggushu (抗骨疏) on osteoporosis in model rats and the underlying mechanisms. Gao, Zhi-ming; Yang, Li; Huang, Feng; Xiong, Ai-hua; Zhou, Ning; Zhou, Lan; Li, Kong-yan; Deng, Jie-li; Li, Kai-yu; Liu, Wei; Chen, Yao; Luo, Wan-ting; Nie, Hong // Chinese Journal of Integrative Medicine;Nov2013, Vol. 19 Issue 11, p844 

    Objective: To investigate the preventive effects and possible underlying mechanism of different extracts of Kanggushu (抗骨疏) on osteoporosis in ovariectomized rats. Methods: One hundred and sixtyfive female SD rats were divided into 11 groups: control, sham, model, Xianling Gubao...

  • Resveratrol Prevents Cardiovascular Complications in the SHR/STZ Rat by Reductions in Oxidative Stress and Inflammation. Vella, Rebecca K.; Pullen, Candice; Coulson, Fiona R.; Fenning, Andrew S. // BioMed Research International;2/23/2015, Vol. 2015, p1 

    The cardioprotective effects of resveratrol are well established in animal models of metabolic disease but are yet to be investigated in a combined model of hypertension and diabetes. This study investigated the ability of resveratrol’s antioxidant and anti-inflammatory effects to prevent...

  • Antithrombotic effect of ligustrazine hydrochloride injection on the model of induced arteriovenous shunt thrombosis. Peng Wang; Chun-hua Luo; Qun-xing Wang; Qian-yuan Li; Peng Li; Xiao-hui Yuan // Journal of Research in Medical Sciences;Aug2013, Vol. 18 Issue 8, p704 

    Background: The objective of this study is to optimize the effective dose of heparin and ligustrazine hydrochloride injection (LHI) for drug combination. Materials and Methods: The animal clinical study of LHI was performed by the rat’s model of induced arteriovenous shunt thrombosis....

  • The Electrophysiological Effects of Qiliqiangxin on Cardiac Ventricular Myocytes of Rats. Yidong Wei; Xiaoyu Liu; Haidong Wei; Lei Hou; Wenliang Che; Erlinda The; Gang Li; Jhummon, Muktanand Vikash; Wanlin Wei // Evidence-based Complementary & Alternative Medicine (eCAM);2013, Vol. 2013, p1 

    Qiliqiangxin, a Chinese herb, represents the affection in Ca channel function of cardiac myocytes. It is unknown whether Qiliqiangxin has an effect on Na current and K current because the pharmacological actions of this herb's compound are very complex. We investigated the rational usage of...

  • Modulation of Brain Electroencephalography Oscillations by Electroacupuncture in a Rat Model of Postincisional Pain. Jing Wang; Xuezhu Li; Duan Li; Xiao-Li Li; Ji-Sheng Han; You Wan // Evidence-based Complementary & Alternative Medicine (eCAM);2013, Vol. 2013, p1 

    The present study aimed to investigate how ongoing brain rhythmical oscillations changed during the postoperative pain and whether electroacupuncture (EA) regulated these brain oscillations when it relieved pain. We established a postincisional pain model of rats with plantar incision to mimic...

  • Long-Term Supplementation with Beta Serum Concentrate (BSC), a Complex of Milk Lipids, during Post-Natal Brain Development Improves Memory in Rats. Jian Guan; MacGibbon, Alastair; Fong, Bertram; Rong Zhang; Liu, Karen; Rowan, Angela; McJarrow, Paul // Nutrients;2015, Vol. 7 Issue 6, p4526 

    We have previously reported that the supplementation of ganglioside-enriched complex-milk-lipids improves cognitive function and that a phospholipid-enriched complex-milk-lipid prevents age-related cognitive decline in rats. This current study evaluated the effects of post-natal supplementation...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics