TITLE

Halk Elinde Yetiştirilen Holştayn, Doğu Anadolu Kırmızısı ve Yerli Kara Sığır Irklarında Diacylglycerol O-Acyltransferase 1 (DGAT1) Gen Polimorfizminin PCR-RFLP Yöntemi ile Belirlenmesi

AUTHOR(S)
BAL, Onur; AKYÜZ, Bilal
PUB. DATE
January 2014
SOURCE
Journal of Faculty of Veterinary Medicine, Erciyes University / ;2014, Vol. 11 Issue 1, p7
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The purpose of this work was to examine the allele structures of DGAT1 gene by RFLP method in 100 head of Holstein, 50 head East Anatolian Red and 50 head Anatolian Black cattle breeds raised in Turkey. In order to determine the DGAT1 alleles, the products obtained from PCR were digested with CfrI endonuclease enzyme. After digestion, a 411 bp single band in KK genotype; three bands (411, 208 and 203 bp) in KA genotype and two bands (208 and 203 bp) in AA genotype were observed. The AA genotypic frequency was found the highest in the Holstein breed; the KK genotypic frequency was found the highest in the Anatolian Black breed and the KA genotypic frequency was found the highest likewise in the Anatolian Black breed in DGAT1 gene. In this study, the A allele frequency was found higher than the K frequency allele in Holstein and East Anatolian Red cattle breeds. But, the K allele frequency was found higher than the A allele in only the Anatolian Black breed. It was found that HW equilibrium was not in DGAT1 locus in all three breeds examined.
ACCESSION #
96053136

 

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