Levels of HOXB7 and miR-337 in pancreatic ductal adenocarcinoma patients

Rui Zhang; Shangen Zheng; Yuwen Du; Yuanyuan Wang; Wenqiao Zang; Guoqiang Zhao
May 2014
Diagnostic Pathology;2014, Vol. 9 Issue 1, p1
Academic Journal
Background Many studies have revealed that homeobox-B7 (HOXB7) and miR-337 play important roles in different types of human cancers. However, the relationship of HOXB7 and miR-337 in PDAC with clinicopathological factors has not yet been examined and their biological roles remain to be explored. Methods Using quantitative real-time RT-PCR and immunohistochemical staining, the expression of HOXB7 mRNA, miR-337, and HOXB7 protein in 44 PDAC samples was detected. Survival curves were made using follow-up data. The relationship between clinical or pathological characteristics and the prognosis was analyzed. Results The expression levels of HOXB7 mRNA and HOXB7 protein were significantly elevated in PDAC samples than that in non-malignant adjacent tissues. There were obvious differences in HOXB7 mRNA and proteins between tumors of different diameters, differentiation, TNM stage, and lymph node status. The level of miR-337 was markedly lower in tumor samples than in non-malignant adjacent tissues. The expression of miR-337 was related to TNM stage and lymph node status. There were significant differences in survival curves between patients with tumors <4 cm in diameter and patients with tumors ≥4 cm, among groups of well, moderately, and poorly differentiated tumors, between groups with TNM stages I, II and III or IV, between groups with metastatic lymph nodes and non-metastatic lymph nodes, among groups of HOXB7 protein expression negative (or weak) and positive, between groups with low levels of miR-337 expression and with high levels of miR-337 expression. The levels of HOXB7 mRNA, HOXB7 protein, and miR-337 were found to be associated with longer survival. Conclusion The present study showed that HOXB7 was over-expressed and miR-337 was minimally expressed in PDAC tissues, and their levels were related to TNM stage and lymph node status. The levels of HOXB7 mRNA, HOXB7 protein, and miR-337 were associated with survival in PDAC patients. Results suggested that HOXB7 and miR-337 could be used as determinants of PDAC patient prognosis. Virtual slides The virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1509730773118658.


Related Articles

  • An analysis of HER-2/neu gene status in invasive ductal carcinomas using immunohistochemistry and fluorescence in situ hybridization. Köseoğlu, Reşit Doğan; Müslehıddınoğlu, Ahmet; Erkorkmaz, Ünal; Caferler, Jülide Saliha; Dürer, Pervin; Özön, Yavuz Hakan // Turkish Journal of Medical Sciences;Oct2011, Vol. 41 Issue 5, p809 

    Aim: To determine the concordance rates between results from immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) assays for the HER-2/neu gene in cases of invasive ductal carcinoma. IHC and FISH are 2 of the most frequently used methods to determine HER-2/neu gene status in...

  • Expression of HOXC8 is inversely related to the progression and metastasis of pancreatic ductal adenocarcinoma. Adwan, H.; Zhivkova-Galunska, M.; Georges, R.; Eyol, E.; Kleeff, J.; Giese, N. A.; Friess, H.; Bergmann, F.; Berger, M. R. // British Journal of Cancer;7/4/2011, Vol. 105 Issue 2, p288 

    Background:The transcription factor HOXC8 regulates many genes involved in tumour progression. This study was to investigate the role of HOXC8 in pancreatic ductal adenocarcinoma (PDAC) growth and metastasis.Methods:The Hoxc8 expression was determined in 15 PDAC cell lines and human specimens by...

  • Sam68 expression and cytoplasmic localization is correlated with lymph node metastasis as well as prognosis in patients with early-stage cervical cancer. Li, Z.; Yu, C.-P.; Zhong, Y.; Liu, T.-J.; Huang, Q.-D.; Zhao, X.-H.; Huang, H.; Tu, H.; Jiang, S.; Zhang, Y.; Liu, J.-H.; Song, L.-B. // Annals of Oncology;Mar2012, Vol. 23 Issue 3, p638 

    Background: This study was aimed at investigating the role and molecular mechanism of Sam68 in cervical cancer lymph node metastasis. Materials and methods: Sam68 expression profile was detected by quantitative polymerase chain reaction, western blotting and immunohistochemical staining. Short...

  • OLFM4 is associated with lymph node metastasis and poor prognosis in patients with gastric cancer. Luo, Zuyan; Zhang, Qi; Zhao, Zhongsheng; Li, Bo; Chen, Junfa; Wang, Yuanyu // Journal of Cancer Research & Clinical Oncology;Nov2011, Vol. 137 Issue 11, p1713 

    Purpose: The present study investigated the clinical significance of the relationship between olfactomedin 4 (OLFM4) expression and the clinicopathological features of patients with gastric cancer. Methods: Tumor tissue and adjacent normal tissue, lymph nodes, and peritoneal metastases were...

  • STAT3 mRNA and protein expression in colorectal cancer: effects on STAT3-inducible targets linked to cell survival and proliferation. Silke Lassmann // Journal of Clinical Pathology;Feb2007, Vol. 60 Issue 2, p173 

    AIMS: To evaluate mRNA and protein expression of signal transducers and activators of transcription (STAT)3 in colorectal carcinomas (CRCs) and to define the association of STAT3 activity with the STAT3-inducible targets cyclin D1, survivin, Bcl-xl and Mcl-1. MATERIALS AND METHODS: Matching...

  • A comprehensive examination of Smad4, Smad6 and Smad7 mRNA expression in pancreatic ductal adenocarcinoma. Singh, P.; Wig, J. D.; Srinivasan, R.; Radotra, B. D. // Indian Journal of Cancer;Apr-Jun2011, Vol. 48 Issue 2, p170 

    BACKGROUND: Smad4, Smad6 and Smad7 are important molecules in TGF-beta pathway, which plays an important role in pancreatic ductal adenocarcinoma (PDAC) biology. AIMS: This study examined the expression profiles of Smad4, Smad6 and Smad7 mRNA in patient samples of PDAC and their relationship to...

  • Cydooxygenase-2 in Barrett's Esophagus, Barrett's Adenocarcinomas, and Esophageal SCC: Ready for Clinical Trials. Lord, Reginald V. N.; Danenberg, Kathleen D.; Danenberg, Peter V.; Johnson, David // American Journal of Gastroenterology;Aug1999, Vol. 94 Issue 8, p2313 

    These studies investigated the role of cyclooxygenase gene expression in esophageal diseases. The study from Germany by Zimmerman et al. detected cytoplasmic cyclooxygenase- 2 (COX-2) protein expression by immunohistochemistry in 156 of 172 (91%) esophageal squamous cell carcinomas...

  • Extrathymic rearrangement of αβT-lymphocyte antigen receptor genes during pregnancy. Shirshev, S. V.; Kuklina, E. M.; A. Yu.Maksimov; Krapivina, O. A.; Parshakova, N. S. // Biochemistry (00062979);Sep2007, Vol. 72 Issue 9, p983 

    The existence of αβT-lymphocyte differentiation processes have been demonstrated in mouse peripheral lymphoid organs during pregnancy. Study of pregnant Swiss mice has shown that the development of the second half of gestation is accompanied by expression of RAG-1 recombinase mRNA and...

  • Down-Regulation of ECRG4, a Candidate Tumor Suppressor Gene, in Human Breast Cancer. Sabatier, Renaud; Finetti, Pascal; Adelaide, José; Guille, Arnaud; Borg, Jean-Paul; Chaffanet, Max; Lane, Lydie; Birnbaum, Daniel; Bertucci, François // PLoS ONE;2011, Vol. 6 Issue 11, p1 

    Introduction: ECRG4/C2ORF40 is a potential tumor suppressor gene (TSG) recently identified in esophageal carcinoma. Its expression, gene copy number and prognostic value have never been explored in breast cancer. Methods: Using DNA microarray and array-based comparative genomic hybridization...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics