Numb Promotes Cell Proliferation and Correlates with Poor Prognosis in Hepatocellular Carcinoma

Wu, Jian; Shen, Shun-Li; Chen, Bin; Nie, Jing; Peng, Bao-Gang
April 2014
PLoS ONE;Apr2014, Vol. 9 Issue 4, p1
Academic Journal
Background: Numb is an evolutionary conserved protein that plays critical roles in cell fate determination, cell adhesion, cell migration and a number of signaling pathways, but evidence for a substantial involvement of Numb in HCC has remained unclear. The present study was aimed to investigate the clinical and prognostic significance of Numb and its role in hepatocellular carcinoma (HCC). Methodology: The expression of Numb was detected in 107 cases of clinical paraffin-embedded hepatocellular carcinoma tissues,5 matched paris of fresh tissues and six hepatocellular cell lines by immunohistochemistry with clinicopathological analyses,RT-PCR or Western blot. Moreover, loss of function and gain of function assays were performed to evaluate the effect of Numb on cell proliferation in vitro. Conclusions: We found that Numb was obviously up-regulated in HCC tissues and cell lines (p<0.05). The Numb up-regulation correlated significantly with poor prognosis, and Numb status was identified as an independent prognostic factor. Over-expression of Numb increased proliferation in SMMC-7721 and BEL-7402 cells, while knock-down of Numb showed the opposite effect. Our study indicates that Numb up-regulation significantly correlates with cell proliferation and poor prognosis in hepatocellular carcinoma patients. It may be a useful biomarker for therapeutic strategy in hepatocellular carcinoma treatment.


Related Articles

  • Expression and clinical significance of Nectin-4 in hepatocellular carcinoma. Jie Ma; ZhiYong Sheng; Yang Lv; WenBin Liu; QiYang Yao; TingTing Pan; ZhiJun Xu; ChuanHai Zhang; GeLiang Xu // OncoTargets & Therapy;Jan2016, Vol. 9, p183 

    Objective: Nectin-4 is a member of the Nectin family of four Ca+-independent immunoglobulin-like cell adhesion molecules and implicated in cell adhesion, movement, proliferation, differentiation, polarization, and survival. The aberrant expression of Nectin-4 has been found in a variety of...

  • Expression of STAT3 and p-STAT3 in human hepatocellular carcinoma and its relationship wtth clinicopathological characteristics. SONG Tao; YANG Xue; GUO Cheng; YAO Ying-ming; YANG Wei; DING Fei-hu; LIU Qing-guang // Journal of Xi'an Jiaotong University (Medical Sciences);Jan2014, Vol. 35 Issue 1, p81 

    Objective To study the expression of signal transducer and activator of transcription 3 (STAT3) and phosphates signal transducer and activator of transcsiption 3 (p-STAT3) in human hepatocellular carcinoma (HCC) tissues and paracancerous tissues and the relationship between p-STAT3 expression...

  • Influence of smoothened siRNA on human esophageal cancer cell line EC9706 proliferation and apoptosis. Wu Hui; Chen Kuisheng; Zhang Hongxin; Ding Yanzhi; Zhao Zhihua; Zhang Hu // Pakistan Journal of Pharmaceutical Sciences;Sep2014, Vol. 27 Issue 5, p1661 

    Apply siRNA technology to take Smoothened (Smo) genetic locus, the possibility of Hh signaling pathway, and also inhibits esophageal squamous carcinoma cell proliferation and induction of apoptosis. Utilizing Smo siRNA transfected esophageal squamous carcinoma EC9706 cells, adopting RT-PCR and...

  • DAX-1 Inhibits Hepatocellular Carcinoma Proliferation by Inhibiting β-Catenin Transcriptional Activity. Jiang, Hong-Lei; Xu, Dong; Yu, Hao; Ma, Xu; Lin, Guo-Fu; Ma, Dong-Yan; Jin, Jun-Zhe // Cellular Physiology & Biochemistry (Karger AG);Aug2014, Vol. 34 Issue 3, p734 

    Background/Aims: Hepatocellular carcinoma (HCC) represents the most common type of liver cancer. DAX1 (dosage-sensitive sex reversal adrenal hypoplasia congenital critical region on X chromosome, gene 1), an atypical member of the nuclear receptor family due to lack of classical DNA-binding...

  • POSTER SESSION: BASIC SCIENCE.  // European Journal of Heart Failure;May2013 Supplement, Vol. 12, pS36 

    No abstract available.

  • The Lin28/let-7a/c-Myc pathway plays a role in non-muscle invasive bladder cancer. Li, Yunfei; Liu, Haitao; Lai, Caiyong; Du, Xinghua; Su, Zexuan; Gao, Shuangquan // Cell & Tissue Research;Nov2013, Vol. 354 Issue 2, p533 

    We investigate the role of the Lin28/let-7a/c-Myc pathway in non-muscle invasive bladder cancer (NMIBC). Using RT-PCR, western blot and immunohistochemistry techniques, the levels of pre-let-7a, let-7a, Lin28 and c-Myc RNA and/or proteins were determined in samples of normal bladder tissue and...

  • Restoration of WNT4 inhibits cell growth in leukemia-derived cell lines. García-Castro, Beatriz; Alvarez-Zavala, Monserrat; Riveros-Magaña, Alma R.; Ortíz-Lazareno, Pablo C.; Ratkovich-González, Sarah; Hernández-Flores, Georgina; Bravo-Cuellar, Alejandro; Jave-Suarez, Luis F.; Aguilar-Lemarroy, Adriana // BMC Cancer;2013, Vol. 13 Issue 1, p1 

    Background WNT signaling pathways are significantly altered during cancer development. Vertebrates possess two classes of WNT signaling pathways: the "canonical" WNT/ß-catenin signaling pathway, and the "non-canonical" pathways including WNT/Ca2+ and WNT/Planar cell polarity [PCP] signaling....

  • MicroRNA-19a mediates gastric carcinoma cell proliferation through the activation of nuclear factor-κB. FAN YANG; HONGJIAN WANG; ZHENYU JIANG; ANXIANG HU; LISHA CHU; YILING SUN; JUNQING HAN // Molecular Medicine Reports;2015, Vol. 12 Issue 4, p5780 

    In gastric carcinoma, the nuclear factor-B (NF-B) signaling pathway is highly active, and the constitutive activation of NF-B prompts malignant cell proliferation. MicroRNAs are considered to be important mediators in the regulation of the NF-B signaling pathway. The present study predominantly...

  • Rho GDP Dissociation Inhibitor Beta Promotes Cell Proliferation and Invasion by Modulating the AKT Pathway in Hepatocellular Carcinoma. Fang, Yang; Yi, Jiang; Lizhi, Lv; Qiucheng, Cai // DNA & Cell Biology;Nov2014, Vol. 33 Issue 11, p781 

    Rho GDP dissociation inhibitor (GDI) beta, (RhoGDI2), has been identified as a proto-oncogene that is upregulated in human cancers, but the role of RhoGDI2 in hepatocellular carcinoma (HCC) remains unclear. In the present study, we investigated the RhoGDI2 expression level in HCC tissues and the...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics