TITLE

The Root Hair Assay Facilitates the Use of Genetic and Pharmacological Tools in Order to Dissect Multiple Signalling Pathways That Lead to Programmed Cell Death

AUTHOR(S)
Kacprzyk, Joanna; Devine, Aoife; McCabe, Paul F.
PUB. DATE
April 2014
SOURCE
PLoS ONE;Apr2014, Vol. 9 Issue 4, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The activation of programmed cell death (PCD) is often a result of complex signalling pathways whose relationship and intersection are not well understood. We recently described a PCD root hair assay and proposed that it could be used to rapidly screen genetic or pharmacological modulators of PCD. To further assess the applicability of the root hair assay for studying multiple signalling pathways leading to PCD activation we have investigated the crosstalk between salicylic acid, autophagy and apoptosis-like PCD (AL-PCD) in Arabidopsis thaliana. The root hair assay was used to determine rates of AL-PCD induced by a panel of cell death inducing treatments in wild type plants treated with chemical modulators of salicylic acid synthesis or autophagy, and in genetic lines defective in autophagy or salicylic acid signalling. The assay demonstrated that PCD induced by exogenous salicylic acid or fumonisin B1 displayed a requirement for salicylic acid signalling and was partially dependent on the salicylic acid signal transducer NPR1. Autophagy deficiency resulted in an increase in the rates of AL-PCD induced by salicylic acid and fumonisin B1, but not by gibberellic acid or abiotic stress. The phenylalanine ammonia lyase-dependent salicylic acid synthesis pathway contributed only to death induced by salicylic acid and fumonisin B1. 3-Methyladenine, which is commonly used as an inhibitor of autophagy, appeared to influence PCD induction in all treatments suggesting a possible secondary, non-autophagic, effect on a core component of the plant PCD pathway. The results suggest that salicylic acid signalling is negatively regulated by autophagy during salicylic acid and mycotoxin-induced AL-PCD. However, this crosstalk does not appear to be directly involved in PCD induced by gibberellic acid or abiotic stress. This study demonstrates that the root hair assay is an effective tool for relatively rapid investigation of complex signalling pathways leading to the activation of PCD.
ACCESSION #
95819432

 

Related Articles

  • Differential Inhibitor Sensitivity between Human Kinases VRK1 and VRK2. Vázquez-Cedeira, Marta; Barcia-Sanjurjo, Iria; Sanz-García, Marta; Barcia, Ramiro; Lazo, Pedro A. // PLoS ONE;2011, Vol. 6 Issue 8, p1 

    Human vaccinia-related kinases (VRK1 and VRK2) are atypical active Ser-Thr kinases implicated in control of cell cycle entry, apoptosis and autophagy, and affect signalling by mitogen activated protein kinases (MAPK). The specific structural differences in VRK catalytic sites make them suitable...

  • Autophagy Plays a Protective Role in Advanced Glycation End Product-Induced Apoptosis in Cardiomyocytes. Hu, Pengfei; Zhou, Hui; Lu, Ming; Dou, Liping; Bo, Gang; Wu, Jiale; Huang, Shuwei // Cellular Physiology & Biochemistry (Karger AG);Sep2015, Vol. 37 Issue 2, p697 

    Background/Aims: To investigate the effect of advanced glycation endproduct-induced autophagy in rat cardiomyocytes and to identify the role of autophagy in advanced glycation end product-induced cell apoptosis. Methods: After cultured rat cardiomyocytes were treated with advanced glycation end...

  • Feeding by Whiteflies Suppresses Downstream Jasmonic Acid Signaling by Eliciting Salicylic Acid Signaling. Zhang, Peng-Jun; Li, Wei-Di; Huang, Fang; Zhang, Jin-Ming; Xu, Fang-Cheng; Lu, Yao-Bin // Journal of Chemical Ecology;May2013, Vol. 39 Issue 5, p612 

    Phloem-feeding whiteflies in the species complex Bemisia tabaci cause extensive crop damage worldwide. One of the reasons for their 'success' is their ability to suppress the effectual jasmonic acid (JA) defenses of the host plant. However, little is understood about the mechanisms underlying...

  • Exogenous Salicylic Acid Activates Two Signaling Arms of the Unfolded Protein Response in Arabidopsis. Nagashima, Yukihiro; Iwata, Yuji; Ashida, Makoto; Mishiba, Kei-ichiro; Koizumi, Nozomu // Plant & Cell Physiology;Oct2014, Vol. 55 Issue 10, p1772 

    The unfolded protein response (UPR) is a highly conserved cellular response that prevents abnormal maturation of proteins in the endoplasmic reticulum (ER). The expression of genes encoding ER chaperones is induced during the UPR. In the Arabidopsis UPR, two membrane-bound transcription factors,...

  • Stability of small ubiquitin-like modifier (SUMO) proteases OVERLY TOLERANT TO SALT1 and -2 modulates salicylic acid signalling and SUMO1/2 conjugation in Arabidopsis thaliana. Bailey, Mark; Srivastava, Anjil; Conti, Lucio; Nelis, Stuart; Cunjin Zhang; Florance, Hannah; Love, Andrew; Milner, Joel; Napier, Richard; Grant, Murray; Sadanandom, Ari // Journal of Experimental Botany;Jan2016, Vol. 67 Issue 1, p353 

    Small ubiquitin-like modifier proteases 1 and 2 (SUMO1/2) have been linked to the regulation of salicylic acid (SA)-mediated defence signalling in Arabidopsis thaliana. In order to define the role of the SUMO proteases OVERLY TOLERANT TO SALT1 and -2 (OTS1/2) in defence and to provide insight...

  • The Saharan isolate Saccharothrix algeriensis NRRL B-24137 induces systemic resistance in Arabidopsis thaliana seedlings against Botrytis cinerea. Muzammil, Saima; Graillon, Clotilde; Saria, Rayenne; Mathieu, Florence; Lebrihi, Ahmed; Compant, Stéphane // Plant & Soil;Jan2014, Vol. 374 Issue 1/2, p423 

    Background and aim: Saccharothrix algeriensis NRRL B-24137, isolated from a Saharan soil, has been described as a potential biocontrol agent against Botrytis cinerea and other phytopathogens. However, the plant protection mechanisms involved still need to be described. The aim of this study was...

  • Mitochondrial-Derived Reactive Oxygen Species Play a Vital Role in the Salicylic Acid Signaling Pathway in Arabidopsis thaliana. Nie, Shengjun; Yue, Haiyun; Zhou, Jun; Xing, Da // PLoS ONE;Mar2015, Vol. 10 Issue 3, p1 

    Plant mitochondria constitute a major source of ROS and are proposed to act as signaling organelles in the orchestration of defense response. At present, the signals generated and then integrated by mitochondria are still limited. Here, fluorescence techniques were used to monitor the events of...

  • Salicylic acid activates nitric oxide synthesis in Arabidopsis. Zottini, Michela; Costa, Alex; De Michele, Roberto; Ruzzene, Maria; Carimi, Francesco; Lo Schiavo, Fiorella // Journal of Experimental Botany;Apr2007, Vol. 58 Issue 6, p1397 

    The relationship between nitric oxide (NO) and salicylic acid (SA) was investigated in Arabidopsis thaliana. Here it is shown that SA is able to induce NO synthesis in a dose-dependent manner in Arabidopsis. NO production was detected by confocal microscopic analysis and spectrofluorometric...

  • Mitogen-Activated Protein Kinase 4 Is a Salicylic Acid-Independent Regulator of Growth But Not of Photosynthesis in Arabidopsis. Gawroński, Piotr; Witoń, Damian; Vashutina, Kateryna; Bederska, Magdalena; Betliński, Błażej; Rusaczonek, Anna; Karpiński, Stanisław // Molecular Plant (Oxford University Press / USA);Jul2014, Vol. 7 Issue 7, p1 

    In this study, we show that Mitogen-Activated Protein Kinase 4 (MPK4) is a complex regulator of chloroplastic retrograde signaling for photosynthesis in Arabidopsis. Our work strongly supports the general hypothesis that light-acclimatory responses, regulation of photosynthesis, as well as...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics