Rhinovirus-16 Induced Release of IP-10 and IL-8 Is Augmented by Th2 Cytokines in a Pediatric Bronchial Epithelial Cell Model

Cakebread, Julie A.; Haitchi, Hans Michael; Xu, Yunhe; Holgate, Stephen T.; Roberts, Graham; Davies, Donna E.
April 2014
PLoS ONE;Apr2014, Vol. 9 Issue 4, p1
Academic Journal
Background: In response to viral infection, bronchial epithelial cells increase inflammatory cytokine release to activate the immune response and curtail viral replication. In atopic asthma, enhanced expression of Th2 cytokines is observed and we postulated that Th2 cytokines may augment the effects of rhinovirus-induced inflammation. Methods: Primary bronchial epithelial cell cultures from pediatric subjects were treated with Th2 cytokines for 24 h before infection with RV16. Release of IL-8, IP-10 and GM-CSF was measured by ELISA. Infection was quantified using RTqPCR and TCID50. Phosphatidyl inositol 3-kinase (PI3K) and P38 mitogen activated protein kinase (MAPK) inhibitors and dexamethasone were used to investigate differences in signaling pathways. Results: The presence of Th2 cytokines did not affect RV replication or viral titre, yet there was a synergistic increase in IP-10 release from virally infected cells in the presence of Th2 cytokines. Release of IL-8 and GM-CSF was also augmented. IP-10 release was blocked by a PI3K inhibitor and IL-8 by dexamethasone. Conclusion: Th2 cytokines increase release of inflammatory cytokines in the presence of rhinovirus infection. This increase is independent of effects of virus replication. Inhibition of the PI3K pathway inhibits IP-10 expression.


Related Articles

  • Lipoteichoic acid upregulates NF-κB and proinflammatory cytokines by modulating β-catenin in bronchial epithelial cells. JAEWOONG JANG; WONYONG KIM; KIJEONG KIM; SANG-IN CHUNG; YAE JIE SHIM; SEOK-MIN KIM; YOOSIK YOON // Molecular Medicine Reports;2015, Vol. 12 Issue 3, p4720 

    Lipoteichoic acid (LTA) is a major cell wall component and virulence factor of gram-positive bacteria. The present study investigated the LTA-induced inflammatory response of BEAS-2B human bronchial epithelial cells, and detected the expression levels of proinflammatory cytokines interleukin...

  • Upregulation of TMEM16A Protein in Bronchial Epithelial Cells by Bacterial Pyocyanin. Caci, Emanuela; Scudieri, Paolo; Di Carlo, Emma; Morelli, Patrizia; Bruno, Silvia; De Fino, Ida; Bragonzi, Alessandra; Gianotti, Ambra; Sondo, Elvira; Ferrera, Loretta; Palleschi, Alessandro; Santambrogio, Luigi; Ravazzolo, Roberto; Galietta, Luis J. V. // PLoS ONE;Jun2015, Vol. 10 Issue 6, p1 

    Induction of mucus hypersecretion in the airway epithelium by Th2 cytokines is associated with the expression of TMEM16A, a Ca2+-activated Cl- channel. We asked whether exposure of airway epithelial cells to bacterial components, a condition that mimics the highly infected environment occurring...

  • Toll-Like Receptors: Role in Inflammation and Commensal Bacteria. Fooladi, Abbas Ali Imani; Mousavi, Seyed Fazlollah; Seghatoleslami, Sepideh; Yazdani, Samaneh; Nourani, Mohammad Reza // Inflammation & Allergy - Drug Targets;Jun2011, Vol. 10 Issue 3, p198 

    TLR ligands are present on both commensal and pathogenic microbes. Intestinal epithelial cells (IECs) have been observed to be largely unresponsive to TLR ligands. This observation has partly been explained by the fact that TLR expression on IECs is sparse. The discovery of the Toll-like...

  • IL-10- and IL-20-Expressing Epithelial and Inflammatory Cells are Increased in Patients with Ulcerative Colitis. Fonseca-Camarillo, Gabriela; Furuzawa-Carballeda, Janette; Llorente, Luis; Yamamoto-Furusho, Jesús // Journal of Clinical Immunology;Apr2013, Vol. 33 Issue 3, p640 

    Interleukin (IL)-20, a pro-inflammatory cytokine, is a recently discovered member of the IL-10 family. This cytokine has been described in inflammatory diseases such as psoriasis and asthma. However, IL-20 expression in ulcerative colitis (UC) patients has not been yet described. The aim of this...

  • Modulation of expression in BEAS-2B airway epithelial cells of α- l-fucosidase A1 and A2 by Th1 and Th2 cytokines, and overexpression of α- l-fucosidase 2. Sobkowicz, Anna; Gallagher, Mary; Reid, Colm; Crean, Daniel; Carrington, Stephen; Irwin, Jane // Molecular & Cellular Biochemistry;May2014, Vol. 390 Issue 1/2, p101 

    Chronic Th2-driven airway inflammation with excessive mucus production occurs in asthma. The regulation of FUCA1 and FUCA2 gene expression and enzyme activity in response to asthma-associated Th2 cytokines and, for contrast, Th1 cytokine IFN-γ, were investigated in a human airway cell line....

  • Resolution of PMA-Induced Skin Inflammation Involves Interaction of IFN-γ and ALOX15. Guojun Zhang; Xiaoman Liu; Chunhui Wang; Liwei Qu; Jingjing Deng; Hui Wang; Zhihai Qin // Mediators of Inflammation;2013, Vol. 2013, p1 

    Background. Acute inflammation and its timely resolution play important roles in the body's responses to the environmental stimulation. Although IFN-γ is well known for the induction of inflammation, its role in the inflammation resolution is still poorly understood. Methodology and Principal...

  • PKC Activation Induces Inflammatory Response and Cell Death in Human Bronchial Epithelial Cells Kim, Hyunhee; Zamel, Ricardo; Bai, Xiao-Hui; Liu, Mingyao // PLoS ONE;May2013, Vol. 8 Issue 5, p1 

    A variety of airborne pathogens can induce inflammatory responses in airway epithelial cells, which is a crucial component of host defence. However, excessive inflammatory responses and chronic inflammation also contribute to different diseases of the respiratory system. We hypothesized that the...

  • The Role of Interleukin-1 and Interleukin-18 in Pro-Inflammatory and Anti-Viral Responses to Rhinovirus in Primary Bronchial Epithelial Cells Piper, Siân C.; Ferguson, John; Kay, Linda; Parker, Lisa C.; Sabroe, Ian; Sleeman, Matthew A.; Briend, Emmanuel; Finch, Donna K. // PLoS ONE;May2013, Vol. 8 Issue 5, p1 

    Human Rhinovirus (HRV) is associated with acute exacerbations of chronic respiratory disease. In healthy individuals, innate viral recognition pathways trigger release of molecules with direct anti-viral activities and pro-inflammatory mediators which recruit immune cells to support viral...

  • Human rhinovirus infection causes different DNA methylation changes in nasal epithelial cells from healthy and asthmatic subjects. McErlean, Peter; Favoreto Jr., Silvio; Costa, Fabricio F.; Junqing Shen; Quraishi, Jihan; Biyasheva, Assel; Cooper, Jocelyn J.; Scholtens, Denise M.; Vanin, Elio F.; de Bonaldo, Maria F.; Hehuang Xie; Soares, Marcelo B.; Avila, Pedro C. // BMC Medical Genomics;2014, Vol. 7 Issue 1, p2 

    Background Mechanisms underlying the development of virus-induced asthma exacerbations remain unclear. To investigate if epigenetic mechanisms could be involved in virus-induced asthma exacerbations, we undertook DNA methylation profiling in asthmatic and healthy nasal epithelial cells (NECs)...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics