TITLE

GPER functions as a tumor suppressor in triple-negative breast cancer cells

AUTHOR(S)
Weißenborn, Christine; Ignatov, Tanja; Ochel, Hans-Joachim; Costa, Serban; Zenclussen, Ana; Ignatova, Zoya; Ignatov, Atanas
PUB. DATE
May 2014
SOURCE
Journal of Cancer Research & Clinical Oncology;May2014, Vol. 140 Issue 5, p713
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: The orphan, membrane-bound estrogen receptor (GPER) is expressed at high levels in a large fraction of breast cancer patients and its expression is favorable for patients' survival. Methods: We investigated the role of GPER as a potential tumor suppressor in triple-negative breast cancer cells MDA-MB-231 and MDA-MB-468 using cell cycle analysis and apoptosis assay. The constitutive activity of GPER was investigated. Results: GPER-specific activation with G-1 agonist inhibited breast cancer cell growth in concentration-dependent manner via induction of the cell cycle arrest in G2/M phase, enhanced phosphorylation of histone H3 and caspase-3-mediated apoptosis. Analysis of the methylation status of the GPER promoter in the triple-negative breast cancer cells and in tissues derived from breast cancer patients revealed that GPER amount is regulated by epigenetic mechanisms and GPER expression is inactivated by promoter methylation. Furthermore, GPER expression was induced by stress factors, such as radiation, and GPER amount inversely correlated with the p53 expression level. Conclusions: Overall, our results establish the protective role in breast cancer tumorigenesis, and the cell surface expression of GPER makes it an excellent potential therapeutic target for triple-negative breast cancer.
ACCESSION #
95516508

 

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