Modulation of expression in BEAS-2B airway epithelial cells of α- l-fucosidase A1 and A2 by Th1 and Th2 cytokines, and overexpression of α- l-fucosidase 2

Sobkowicz, Anna; Gallagher, Mary; Reid, Colm; Crean, Daniel; Carrington, Stephen; Irwin, Jane
May 2014
Molecular & Cellular Biochemistry;May2014, Vol. 390 Issue 1/2, p101
Academic Journal
Chronic Th2-driven airway inflammation with excessive mucus production occurs in asthma. The regulation of FUCA1 and FUCA2 gene expression and enzyme activity in response to asthma-associated Th2 cytokines and, for contrast, Th1 cytokine IFN-γ, were investigated in a human airway cell line. BEAS-2B cells were supplemented with Th2-derived cytokines (IL-13, IL-4, IL-5) or/and IFN-γ. RNA and cell supernatants from stimulated and unstimulated cells were collected over a period of 3 h. Alpha- l-fucosidase A1 and A2 gene expression were assessed using real time RT-PCR, while enzymatic activities were measured using a fluorescent assay. To characterise α- l-fucosidase A2, CHO-K1 and BEAS-2B cell lines were transiently transfected, the FUCA2 gene was overexpressed, and the protein was immunoprecipitated. The transcription of FUCA1 was upregulated ( p < 0.01) in response to IFN-γ, suggesting that FUCA1 transcription and fucosidase activity are regulated in a Th1-dependent manner. The gene expression was the highest for 30 min after IFN-γ stimulation (>twofold induction), whereas secreted enzyme activity in BEAS-2B cells was significantly increased 1 h after IFN-γ addition. IL-4, IL-5 and IL-13 had no effect on FUCA1 and FUCA2 expression and activity. The IFN-γ-induced increase in expression and activity was repressed by the presence of the Th2 cytokine IL-5. Enzymatically active α- l-fucosidase 2 was immunoprecipitated from BEAS-2B cells, with highest activity at pH 4.9. IL-13, IL-4 and IL-5 have no effect on the expression of FUCA1 and FUCA2, but its expression is upregulated by IFN-γ, a Th1 cytokine. Active α- l-fucosidase 2 was overexpressed in BEAS-2B cells.


Related Articles

  • High density of IL-17-producing cells is associated with improved prognosis for advanced epithelial ovarian cancer. Lan, Chunyan; Huang, Xin; Lin, Suxia; Huang, Huiqiang; Cai, Qichun; Lu, Jiabin; Liu, Jihong // Cell & Tissue Research;May2013, Vol. 352 Issue 2, p351 

    Interleukin (IL)-17 is the signature cytokine of T helper 17 cells. The role of IL-17 in the tumor microenvironment is still controversial. Few studies describing IL-17 expression in ovarian cancer have been reported. We have therefore analyzed the in situ tumor expression of IL-17 in advanced...

  • Rhinovirus-16 Induced Release of IP-10 and IL-8 Is Augmented by Th2 Cytokines in a Pediatric Bronchial Epithelial Cell Model. Cakebread, Julie A.; Haitchi, Hans Michael; Xu, Yunhe; Holgate, Stephen T.; Roberts, Graham; Davies, Donna E. // PLoS ONE;Apr2014, Vol. 9 Issue 4, p1 

    Background: In response to viral infection, bronchial epithelial cells increase inflammatory cytokine release to activate the immune response and curtail viral replication. In atopic asthma, enhanced expression of Th2 cytokines is observed and we postulated that Th2 cytokines may augment the...

  • Regulation of CXCR/IL-8 in Human Airway Epithelial Cells. Gras, Delphine; Tiers, Laurent; Vachier, Isabelle; de Senneville, Laure Denis; Bourdin, Arnaud; Godard, Philippe; Lehman, Sylvain; Chanez, Pascal // International Archives of Allergy & Immunology;2010, Vol. 152 Issue 2, p140 

    Background: Severe asthma is characterized by neutrophilic inflammation and high levels of interleukin (IL)-8. Airway epithelial cells play a pivotal role in the pathogenesis and chronicity of asthma. The objective of this work was to determine whether CXC receptors were involved in human small...

  • Increased Il-4 Mrna expression and poly-aromatic hydrocarbon concentrations from children with asthma. Al-Daghri, Nasser M.; Abd-Alrahman, Sherif; Draz, Hossam; Alkharfy, Khalid; Khader Mohammed, Abdul; Clerici, Mario S.; Alokail, Majed S. // BMC Pediatrics;2014, Vol. 14 Issue 1, p1 

    Background Asthma is the most common chronic childhood disease. Imbalance of cytokines released from T helper cells and environmental factors, such as exposure to poly-aromatic hydrocarbon (PAH), play pivotal roles in the pathogenesis of asthma. The aim of this study was to compare the mRNA...

  • Regulation of the Development of Asthmatic Inflammation by In Situ CD4Foxp3 T Cells in a Mouse Model of Late Allergic Asthma. Nakashima, Tomomi; Hayashi, Toshiharu; Mizuno, Takuya // Inflammation;Oct2014, Vol. 37 Issue 5, p1642 

    CD4Foxp3T cells (Tregs) mediate homeostatic peripheral tolerance by suppressing helper T2 cells in allergy. However, the regulation of asthmatic inflammation by local ( in situ) Tregs in asthma remains unclear. BALB/c mice sensitized and challenged with ovalbumin (OVA) (asthma group) developed...

  • Effects of Interleukin-31 on MUC5AC Gene Expression in Nasal Allergic Inflammation. Shah, Said ahmad; Ishinaga, Hajime; Hou, Bo; Okano, Mitsuhiro; Takeuchi, Kazuhiko // Pharmacology;2013, Vol. 91 Issue 3/4, p158 

    Background: Interleukin-31 (IL-31) is a newly discovered T helper lymphocyte-derived cytokine that plays an important role in allergic inflammation. However, the effects of IL-31 on mucus production in nasal allergic inflammation are completely unknown. Objective: To investigate the effects of...

  • Lentiviral-mediated GATA-3 RNAi Decreases Allergic Airway Inflammation and Hyperresponsiveness. Chen-Chen Lee; Hsin-Ying Huang; Bor-Luen Chiang // Molecular Therapy;Jan2008, Vol. 16 Issue 1, p60 

    GATA-3 is the key transcriptional factor for Th2 commitment in T cells and is strongly associated with asthma and allergic disease. We studied the silencing of the GATA-3 gene expression using RNA interference (RNAi) delivered by a lentiviral vector, to evaluate the therapeutic role of GATA-3...

  • Active Immunization with Extracellular Vesicles Derived from Staphylococcus aureus Effectively Protects against Staphylococcal Lung Infections, Mainly via Th1 Cell-Mediated Immunity. Choi, Seng Jin; Kim, Min-Hye; Jeon, Jinseong; Kim, Oh Youn; Choi, Youngwoo; Seo, Jihye; Hong, Sung-Wook; Lee, Won-Hee; Jeon, Seong Gyu; Gho, Yong Song; Jee, Young-Koo; Kim, Yoon-Keun // PLoS ONE;9/2/2015, Vol. 10 Issue 9, p1 

    Staphylococcus aureus is an important pathogenic bacterium that causes various infectious diseases. Extracellular vesicles (EVs) released from S. aureus contain bacterial proteins, nucleic acids, and lipids. These EVs can induce immune responses leading to similar symptoms as during...

  • Differential effects of simvastatin on IL-13-induced cytokine gene expression in primary mouse tracheal epithelial cells. Zeki, Amir A.; Thai, Phil; Kenyon, Nicholas J.; Wu, Reen // Respiratory Research;May2012, Vol. 13 Issue 5, p1 

    Background: Asthma causes significant morbidity worldwide in adults and children alike, and incurs large healthcare costs. The statin drugs, which treat hyperlipidemia and cardiovascular diseases, have pleiotropic effects beyond lowering cholesterol, including immunomodulatory,...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics