Hydroxytyrosol stimulates lipolysis via A-kinase and extracellular signal-regulated kinase activation in 3T3-L1 adipocytes

Drira, Riadh; Sakamoto, Kazuichi
April 2014
European Journal of Nutrition;Apr2014, Vol. 53 Issue 3, p743
Academic Journal
Purpose: The principal function of the adipose tissue is the storage of energy in the form of triglyceride through the process of adipogenesis, as well as the provision of the stored energy through lipolysis. In the present study, we investigated the effect of hydroxytyrosol on lipolysis in 3T3-L1 adipocytes. Methods: 3T3-L1 adipocytes, used as in vitro model in this study, were treated with several concentration of hydroxytyrosol. Glycerol release was measured to identify the lipolytic rate activation. All factors activation and expression were carried out via Western blotting and qRT-PCR. Results: Our results showed that hydroxytyrosol, over a range of concentrations, attenuated triglyceride accumulation and stimulated glycerol release in fully differentiated adipocytes in a dose- and time-dependent manner. Moreover, hydroxytyrosol had no effect on adipocyte viability. To understand the mechanism underlying hydroxytyrosol-stimulated lipolysis, we used inhibitors of PKA, PKC, PKG, ERK1/2, and nitric oxide production. Pretreatment with a PKA inhibitor (Rp-cAMPs) and an ERK1/2 inhibitor (U0126) significantly attenuated hydroxytyrosol-stimulated lipolysis. In contrast, a PKC inhibitor (Calphostin C), 2 PKG inhibitors (KT 5823 and Rp-cGMPs), and a nitric oxide inhibitor (S-ethyl ITU) had no effect on hydroxytyrosol-stimulated lipolysis. Over the same range of concentrations, hydroxytyrosol downregulated the expression of adipose triglyceride lipase, hormone sensitive lipase (HSL), and adipogenesis-related transcription factors PPARγ and C/EBPα. In addition, hydroxytyrosol increased the phosphorylation rate of HSL at Ser563 and Ser660, as well as perilipin and ERK phosphorylation. Conclusion: Hydroxytyrosol induced lipolysis in 3T3-L1 adipocytes via the activation of PKA and ERK1/2 pathway.


Related Articles

  • Activation of MITF by Argan Oil Leads to the Inhibition of the Tyrosinase and Dopachrome Tautomerase Expressions in B16 Murine Melanoma Cells. Villareal, Myra O.; Sayuri Kume; Thouria Bourhim; Bakhtaoui, Fatima Zahra; Kenichi Kashiwagi; Junkyu Han; Chemseddoha Gadhi; Hiroko Isoda // Evidence-based Complementary & Alternative Medicine (eCAM);2013, Vol. 2013, p1 

    Argan (Argania spinosa L.) oil has been used for centuries in Morocco as cosmetic oil to maintain a fair complexion and to cure skin pimples and chicken pox pustules scars. Although it is popular, the scientific basis for its effect on the skin has not yet been established. Here, the...

  • Tetrandrine Inhibits the Wnt/β-Catenin Signalling Pathway and Alleviates Osteoarthritis: An In Vitro and In Vivo Study. Xindie Zhou; Weijun Li; Lifeng Jiang; Jiapeng Bao; Lijiang Tao; Jin Li; Lidong Wu // Evidence-based Complementary & Alternative Medicine (eCAM);2013, Vol. 2013, p1 

    There is currently no effective drug treatment for the early phase of osteoarthritis (OA), one of the most common senile diseases. The goal of this study was to investigate the protective effect of the tetrandrine (Tet) on OA, in vitro and in vivo. In an in vitro experiment, quantitative...

  • Ras Oncogene-Mediated Progressive Silencing of Extracellular Superoxide Dismutase in Tumorigenesis. Cammarota, Francesca; de Vita, Gabriella; Salvatore, Marco; Laukkanen, Mikko O. // BioMed Research International;10/15/2015, Vol. 2015, p1 

    Extracellular superoxide dismutase (SOD3) is a secreted enzyme that uses superoxide anion as a substrate in a dismutase reaction that results in the formation of hydrogen peroxide. Both of these reactive oxygen species affect growth signaling in cells. Although SOD3 has growth-supporting...

  • Pesticides and Health in Vegetable Production in Kenya. Macharia, Ibrahim // BioMed Research International;12/10/2015, Vol. 2015, p1 

    This paper investigates the determinants of pesticide-related cost of illness (COI) and acute symptoms, using a balanced panel of 363 farmers interviewed from seven major vegetable producing districts of Kenya. Finding shows that the incidences of pesticide-related health impairments have...

  • Obesity Promotes Alterations in Iron Recycling. Citelli, Marta; Fonte-Faria, Thaís; Nascimento-Silva, Vany; Renovato-Martins, Mariana; Silva, Raphael; Luna, Aderval Severino; Vargas da Silva, Simone; Barja-Fidalgo, Christina // Nutrients;2015, Vol. 7 Issue 1, p336 

    Hepcidin is a key hormone that induces the degradation of ferroportin (FPN), a protein that exports iron from reticuloendothelial macrophages and enterocytes. The aim of the present study was to experimentally evaluate if the obesity induced by a high-fat diet (HFD) modifies the expression of...

  • Regulation of rat hepatic α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase, a key enzyme in the tryptophan-NAD pathway, by dietary cholesterol and sterol regulatory element-binding protein-2. Matsuda, Hiroko; Sato, Mayumi; Yakushiji, Mako; Koshiguchi, Manami; Hirai, Shizuka; Egashira, Yukari // European Journal of Nutrition;Mar2014, Vol. 53 Issue 2, p469 

    Purpose: Nicotinic acid is one of the older drugs used to treat hyperlipidemia, the greatest risk factor of coronary heart disease. Nicotinic acid is also a precursor of the coenzyme nicotinamide adenine dinucleotide (NAD). In mammals, α-amino-β-carboxymuconate-ε-semialdehyde...

  • Immunomodulatory Activity of Red Ginseng against Influenza A Virus Infection. Jong Seok Lee; Hye Suk Hwang; Eun-Ju Ko; Yu-Na Lee; Young-Man Kwon; Min-Chul Kim; Sang-Moo Kang // Nutrients;Feb2014, Vol. 6 Issue 2, p517 

    Ginseng herbal medicine has been known to have beneficial effects on improving human health. We investigated whether red ginseng extract (RGE) has preventive effects on influenza A virus infection in vivo and in vitro. RGE was found to improve survival of human lung epithelial cells upon...

  • Traditional Korean Herbal Formula Samsoeum Attenuates Adipogenesis by Regulating the Phosphorylation of ERK1/2 in 3T3-L1 Cells. Jeong, Soo-Jin; Yoo, Sae-Rom; Seo, Chang-Seob; Shin, Hyeun-Kyoo // Evidence-based Complementary & Alternative Medicine (eCAM);9/21/2015, Vol. 2015, p1 

    Adipogenesis is the cell differentiation process from preadipocytes into adipocytes and the critical action in the development of obesity. In the present study, we conducted in vitro analyses to investigate the inhibitory effects of Samsoeum (SSE), a traditional herbal decoction. SSE had no...

  • Guava leaf extracts promote glucose metabolism in SHRSP.Z-Leprfa/Izm rats by improving insulin resistance in skeletal muscle. Xiangyu Guo; Hisae Yoshitomi; Ming Gao; Lingling Qin; Ying Duan; Wen Sun; Tunhai Xu; Peifeng Xie; Jingxin Zhou; Liansha Huang; Tonghua Liu // BMC Complementary & Alternative Medicine;2013, Vol. 13 Issue 1, p52 

    Background: Metabolic syndrome (MS) and type 2 diabetes mellitus (T2DM) have been associated with insulin-resistance; however, the effective therapies in improving insulin sensitivity are limited. This study is aimed at investigating the effect of Guava Leaf (GL) extracts on glucose tolerance...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics