Effect of 3D Chondrocyte Culturing Conditions on the Formation of Extracellular Matrix in Cartilage Tissue-Engineering Constructs

Ponomarev, I.; Kochneva, L.; Barnewitz, D.
February 2014
Bulletin of Experimental Biology & Medicine;Feb2014, Vol. 156 Issue 4, p548
Academic Journal
We studied biochemical, morphological, and histological parameters of the extracellular matrix in scaffold-free tissue engineering chondrotransplants prepared from chondrocytes isolated from knee joint cartilage biopsy specimens of Haflinger horses (age 3.5-14 years) and in transplants prepared on the basis of commercial matrixes of Ethisorbff and Chondro-Gideff. A total of 50 tissue-engineering constructs were designed and analyzed. Passage 2 cells populations were used. Mechanical stimulation during culturing of scaffold-free constructs considerably activated synthesis of the basic components of the cartilage matrix (proteoglycan concentration was 35% of the content in the native tissue, and the content of collagen-specific amino acids hydroxyproline and hydroxylysine attained 29.3 and 12.7%, respectively). The architecture of these cartilage constructs was morphologically and histologically similar to the native cartilage tissue. Insufficient support of the chondrogenesis by scaffold-based chondrotransplants and no differences between these constructs by the studied parameters were noted despite different chemical nature and structure of these scaffolds.


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