TITLE

Efficient in vitro plant regeneration from immature embryos of endemic Iris sari and I. schachtii

AUTHOR(S)
UZUN, Satı; İLBAŞ, Ali İrfan; İPEK, Arif; ARSLAN, Neşet; BARPETE, Surendra
PUB. DATE
June 2014
SOURCE
Turkish Journal of Agriculture & Forestry;Jun2014, Vol. 38 Issue 3, p348
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Plant tissue culture is an efficient technique for conserving endemic plant species. A reproducible in vitro regeneration protocol was developed for the endemic Iris sari and Iris schachtii in the present study. The highest number of shoots per explant was obtained on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L thidiazuron (TDZ) plus 0.5 mg/L a-naphthaleneacetic acid (NAA) and 1 mg/L TDZ plus 0.5 mg/L NAA, whereby 96.88% and 100% shoot induction with 9.55 and 11.34 shoots per explant of Iris sari and Iris schachtii were recorded, respectively. Regenerated shoots were successfully rooted on MS medium with either 1 mg/L indole-3-butyric acid (IBA) or 1 mg/L IBA plus 0.2 mg/L NAA. Rooted shoots were transferred to pots containing either a peat-soil-sand (1:1:1) mixture or a hydroponic culture containing Hoagland solution to acclimatize the regenerated plants to the greenhouse chamber. Approximately 90% of plants were transferred ex vitro successfully.
ACCESSION #
94828523

 

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics