TITLE

Long noncoding RNA expression signatures of bladder cancer revealed by microarray

AUTHOR(S)
YI-PING ZHU; XIAO-JIE BIAN; DING-WEI YE; XU-DONG YAO; SHI-LIN ZHANG; BO DAI; HAI-LIANG ZHANG; YI-JUN SHEN
PUB. DATE
April 2014
SOURCE
Oncology Letters;2014, Vol. 7 Issue 4, p1197
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Dysregulation of long noncoding RNAs (lncRNAs) has been regarded as a primary feature of several human cancers. However, the genome-wide expression and functional significance of lncRNAs in bladder cancer remains unclear. The aim of this study was to identify aberrantly expressed lncRNAs that may play an important role in contributing to bladder cancer pathogenesis. In this study, we described lncRNAs profiles in four pairs of human bladder cancer and matched normal bladder tissues by microarray. We finally determined 3,324 differentially expressed human lncRNAs and 2,120 differentially expressed mRNAs (≥2-fold change). A total of 110 lncRNAs were significantly differentially expressed between the tumor and the control groups (≥8-fold change). Four lncRNAs (TNXA, CTA-134P22.2, CTC-276P9.1 and KRT19P3) were selected for further confirmation of microarray results using quantitative PCR (qPCR), and a strong correlation was identified between the qPCR results and microarray data. We also observed that numerous lncRNA expression levels were significantly correlated with the expression of tens of protein coding genes by construction of the lncRNA-mRNA co-expression network. Kyoto Encyclopedia of Genes and Genomes annotation showed a significant association with p53, bladder cancer, cell cycle and propanoate metabolism pathway gene expression in the bladder cancer group compared with the normal tissue group, indicating that deregulated lncRNAs may act by regulating protein-coding genes in these pathways. We demonstrated the expression profiles of human lncRNAs in bladder cancer by microarray. We identified a collection of aberrantly expressed lncRNAs in bladder cancer compared with matched normal tissue. It is likely that these deregulated lncRNAs play a key or partial role in the development and/or progression of bladder cancer.
ACCESSION #
94754593

 

Related Articles

  • Distinct Expression Profiles of lncRNAs Between Regressive and Mature Scars. Li, Jingyun; Long, Wei; Li, Qian; Zhou, Qing; Wang, Yu; Wang, Hui; Zhou, Bei; Li, Jun // Cellular Physiology & Biochemistry (Karger AG);Jan2015, Vol. 35 Issue 2, p663 

    Background: Recent studies suggest that long non-coding RNAs (lncRNAs) play crucial roles in human diseases. The function of lncRNAs in abnormal scar pathogenesis remains poorly understood. Methods: In this study, we examined the lncRNAs expression profiles among regressive and mature scars...

  • Dynamic Transcription of Long Non-Coding RNA Genes during CD4+ T Cell Development and Activation. Xia, Fei; Dong, Fulu; Yang, Yi; Huang, Anfei; Chen, Si; Sun, Di; Xiong, Sidong; Zhang, Jinping // PLoS ONE;Jul2014, Vol. 9 Issue 7, p1 

    Background: Recent evidence shows that long non-coding RNA (LncRNA) play important regulatory roles in many biology process, including cell development, activation and oncogenesis. However, the roles of these LncRNAs in the development and activation of CD4+ T cells, which is an important...

  • Long non-coding RNA gadd7 interacts with TDP-43 and regulates Cdk6 mRNA decay. Liu, Xuefeng; Li, Dan; Zhang, Weimin; Guo, Mingzhou; Zhan, Qimin // EMBO Journal;11/28/2012, Vol. 31 Issue 23, p4415 

    Long non-coding RNAs (lncRNAs) transcribed extensively from the genome have been proposed to be key regulators of diverse biological processes. However, little is known about the role of lncRNAs in regulation of the cell-cycle G1/S checkpoint following DNA damage, a key step in the maintenance...

  • Interplay of noncoding RNAs, mRNAs, and proteins during the growth of eukaryotic cells. Zhdanov, V. // Journal of Experimental & Theoretical Physics;Oct2010, Vol. 111 Issue 4, p699 

    Numerous biological functions of noncoding RNAs (ncRNAs) in eukaryotic cells are based primarily on their ability to pair with target mRNAs and then either to prevent translation or to result in rapid degradation of the mRNA-ncRNA complex. Using a general model describing this scenario, we show...

  • A Feed-Forward Regulatory Loop between HuR and the Long Noncoding RNA HOTAIR Promotes Head and Neck Squamous Cell Carcinoma Progression and Metastasis. Cheng-Zhi Xu; Chenyan Jiang; Qingwei Wu; Liu Liu; Xiaojun Yan; Runjie Shi // Cellular Physiology & Biochemistry (Karger AG);Dec2016, Vol. 40 Issue 3/4, p1039 

    Background/Aims: The lncRNA Homeobox (HOX) transcript antisense RNA (HOTAIR) is overexpressed in numerous cancers. HuR is also overexpressed during tumourigenesis and is abnormally present within the cytoplasm, where it binds to AU-rich elements in the 3′UTRs of target mRNA and...

  • Expression Profiles of Long Noncoding RNAs and Messenger RNAs in Mn-Exposed Hippocampal Neurons of Sprague–Dawley Rats Ascertained by Microarray: Implications for Mn-Induced Neurotoxicity. Ma, Shuyan; Qing, Li; Yang, Xiaobo; Liang, Guiqiang; Zhang, Li’e; Li, Qin; Xiong, Feng; Peng, Suwan; Ma, Yifei; Huang, Xiaowei; Zou, Yunfeng // PLoS ONE;1/8/2016, Vol. 11 Issue 1, p1 

    Manganese (Mn) is an essential trace element, while excessive expose may induce neurotoxicity. Recently, lncRNAs have been extensively studied and it has been confirmed that lncRNAs participate in neural functions and aberrantly expressed lncRNAs are involved in neurological diseases. However,...

  • Genome-Wide Analysis of Long Noncoding RNA (lncRNA) Expression in Hepatoblastoma Tissues. Dong, Rui; Jia, Deshui; Xue, Ping; Cui, Ximao; Li, Kai; Zheng, Shan; He, Xianghuo; Dong, Kuiran // PLoS ONE;Jan2014, Vol. 9 Issue 1, p1 

    Long noncoding RNAs (lncRNAs) have crucial roles in cancer biology. We performed a genome-wide analysis of lncRNA expression in hepatoblastoma tissues to identify novel targets for further study of hepatoblastoma. Hepatoblastoma and normal liver tissue samples were obtained from hepatoblastoma...

  • Expression Pattern of Long Non-Coding RNAs in Renal Cell Carcinoma Revealed by Microarray. Qin, Chao; Han, Zhijian; Qian, Jian; Bao, Meiling; Li, Pu; Ju, Xiaobing; Zhang, Shaobo; Zhang, Lei; Li, Shuang; Cao, Qiang; Lu, Qiang; Li, Jie; Shao, Pengfei; Meng, Xiaoxin; Zhang, Wei; Yin, Changjun // PLoS ONE;Jun2014, Vol. 9 Issue 6, p1 

    Background: Recent large-scale transcriptome analyses have found large numbers of transcripts, including that of long non-coding RNAs (lncRNAs), which are aberrant in various diseases, especially cancers. However, it is not clear whether lncRNAs are involved specifically in renal cell carcinoma...

  • Matrin 3 Binds and Stabilizes mRNA. Salton, Maayan; Elkon, Ran; Borodina, Tatiana; Davydov, Aleksey; Yaspo, Marie-Laure; Halperin, Eran; Shiloh, Yosef // PLoS ONE;2011, Vol. 6 Issue 8, p1 

    Matrin 3 (MATR3) is a highly conserved, inner nuclear matrix protein with two zinc finger domains and two RNA recognition motifs (RRM), whose function is largely unknown. Recently we found MATR3 to be phosphorylated by the protein kinase ATM, which activates the cellular response to double...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics