Downregulation of L1CAM inhibits proliferation, invasion and arrests cell cycle progression in pancreatic cancer cells in vitro

April 2014
Experimental & Therapeutic Medicine;2014, Vol. 7 Issue 4, p785
Academic Journal
The aim of the present study was to establish the effect of silencing L1 cell adhesion molecule (L1CAM) on the proliferation, invasion, cell cycle progression and apoptosis of pancreatic cancer cells, and to determine the potential molecular mechanisms that are involved. The human Capan-2 pancreatic cancer cell line was infected with lentivirus-mediated short hairpin RNA (shRNA) to target L1CAM. Cell proliferation and invasion were analyzed using cell counting kit-8 and Transwell assays, respectively, and cell cycle progression and apoptosis were analyzed using flow cytometry. L1CAM protein expression in Capan-2 cells decreased following shRNA-L1CAM infection. Furthermore, knockdown of L1CAM significantly inhibited cell proliferation and reduced the number of invasive cells, while increasing the percentage of cells in the G0/G1 phase (P<0.05). However, the effect on apoptosis was not identified to be statistically significant. In addition, L1CAM silencing may induce activation of p38/extracellular signal regulated kinase1/2. Downregulation of L1CAM may inhibit proliferation, invasion and arrests cell cycle progression in pancreatic cancer via p38/ERK1/2 signal pathway, and therefore, L1CAM may serve as a potential target for gene therapy in pancreatic cancer.


Related Articles

  • Umbilical Cord Blood-Derived Stromal Cells Regulate Megakaryocytic Proliferation and Migration Through SDF-1/PECAM-1 Pathway. Feng, Yimei; Zou, Zhongmin; Gao, Lei; Zhang, Xi; Wang, Tao; Sun, Haoping; Liu, Ying; Chen, Xinghua // Cell Biochemistry & Biophysics;Sep2012, Vol. 64 Issue 1, p5 

    We have previously reported that human umbilical cord blood-derived stromal cells (hUCBDSCs) are able to enhance the expansion of CFU-Meg in vitro, particularly promote the megakaryocytic lineage recovery, and effectively protect the survival of irradiated mice. In this study, we demonstrated...

  • B7-H4's Role 'Beyond the Tumor'. Yu, Ning; Li, Xiaomei; Zheng, Songguo; Li, Xiangpei // Inflammation;Aug2013, Vol. 36 Issue 4, p941 

    B7-H4 is a ligand in the B7 costimulatory family, executing suppressive function on the immune system in many diseases, such as cancer, allograft rejection, and autoimmune diseases. The receptor for this molecule has yet to be clarified. The engagement of B7-H4 inhibits proliferation of immune...

  • EpCAM proteolysis: new fragments with distinct functions? SCHNELL, Ulrike; KUIPERS, Jeroen; GIEPMANS, Ben N. G. // Bioscience Reports;2013, Vol. 33 Issue 2, preceding p321 

    EpCAM [epithelial cell adhesion molecule; CD326 (cluster of differentiation 326)] is highly expressed on epitheliumderived tumours and can play a role in cell proliferation. Recently, RIP (regulated intramembrane proteolysis) has been implicated as the trigger for EpCAM-mediated proliferative...

  • Current Study on HPV16 E5 Protein in Cervical Cancer. YU Yu; ZHANG Shu-lan // Journal of International Obstetrics & Gynecology;Feb2014, Vol. 41 Issue 1, p39 

    HPV16 virus is the most correlative HPV type to cervical cancer and Encoding E5 protein. HPV16 E5 protein as one of membrane integration of proteins, mainly distributed in the golgi apparatus and endoplasmic reticulum. Numerous studies have demonstrated that HPV16 E5 protein regulates cervical...

  • Vitamin D3 up-regulated protein 1 controls the priming phase of liver regeneration. Hyo-Jung Kwon; Sung-Kuk Hong; Won-Kee Yoon; Ki-Hoan Nam; In-Pyo Choi; Dae-Yong Kim; Hyoung-Chin Kim; Young-Suk Won // Journal of Veterinary Science;Sep2013, Vol. 14 Issue 3, p257 

    Vitamin D3 up-regulated protein 1 (VDUP1) is a potent growth suppressor that inhibits tumor cell proliferation and cell cycle progression when overexpressed. In a previous study, we showed that VDUP1 knockout (KO) mice exhibited accelerated liver regeneration because such animals could...

  • Novel FTS-diamine/cinnamic acid hybrids inhibit tumor cell proliferation and migration and promote apoptosis via blocking Ras-related signaling in vitro. Ling, Yong; Zhao, Xinmei; Li, Xianghua; Wang, Xuemin; Yang, Yang; Wang, Zhiqiang; Wang, Xinyang; Zhang, Jie; Zhang, Yihua // Cancer Chemotherapy & Pharmacology;Feb2015, Vol. 75 Issue 2, p381 

    Novel FTS-diamine/cinnamic acid hybrids 7a- f were prepared, and their in vitro biological activities were evaluated. It was found that 7c showed the strongest anti-proliferation activities against cancer cells in vitro and significant growth inhibition of tumor in vivo, and more potential for...

  • Epithelial membrane protein 3 functions as an oncogene and is regulated by microRNA-765 in primary breast carcinoma. XIAO CHUN HONG; YUAN JIAN FEN; GUO CHUN YAN; HONG HONG; CAO HONG YAN; LI WEI BING; YU HAI ZHONG // Molecular Medicine Reports;2015, Vol. 12 Issue 5, p6445 

    Epithelial membrane protein 3 (EMP3) is a transmembrane signaling molecule, which is important in the regulation of apoptosis, differentiation and invasion of cancer cells. However, the specific function and regulatory mechanism of EMP3 in primary breast carcinoma remain to be elucidated. In the...

  • Knockdown of RON Inhibits AP-1 Activity and Induces Apoptosis and Cell Cycle Arrest Through the Modulation of Akt/FoxO Signaling in Human Colorectal Cancer Cells. Chung, Cho-Yun; Park, Young-Lan; Song, Young-A; Myung, Eun; Kim, Kyu-Yeol; Lee, Gi-Hoon; Ki, Ho-Seok; Park, Kang-Jin; Cho, Sung-Bum; Lee, Wan-Sik; Jung, Young-Do; Kim, Kyung-Keun; Joo, Young-Eun // Digestive Diseases & Sciences;Feb2012, Vol. 57 Issue 2, p371 

    Background/Aims: Altered Recepteur d'Origine nantais (RON) expression transduces signals inducting invasive growth phenotype that includes cell proliferation, migration, matrix invasion, and protection of apoptosis in human cancer cells. The aims of the current study were to evaluate whether RON...

  • Different Vascular Smooth Muscle Cell Apoptosis in the Human Internal Mammary Artery and the Saphenous Vein. Frischknecht, Karin; Greutert, Helen; Weisshaupt, Christian; Kaspar, Mathias; Yang, Zhihong; L├╝scher, Thomas F.; Carrel, Thierry P.; Tanner, Felix C. // Journal of Vascular Research;2006, Vol. 43 Issue 4, p338 

    Background: The remarkable patency of internal mammary artery (MA) grafts compared to saphenous vein (SV) grafts has been related to different biological properties of the two blood vessels. We examined whether proliferation and apoptosis of vascular smooth muscle cells (VSMC) from human...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics