TITLE

Mouse induced pluripotent stem cell microenvironment generates epithelial-mesenchymal transition in mouse Lewis lung cancer cells

AUTHOR(S)
Ling Chen; Akifumi Mizutani; Tomonari Kasai; Ting Yan; Guoliang Jin; Vaidyanath, Arun; El-Aarag, Bishoy Y. A.; Yixin Liu; Takayuki Kudoh; Salomon, David S.; Li Fu; Masaharu Seno
PUB. DATE
January 2014
SOURCE
American Journal of Cancer Research;2014, Vol. 4 Issue 1, p80
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Induced pluripotent stem (iPS) cells may be a powerful tool in regenerative medicine, but their potential tumorigenicity is a significant challenge for the clinical use of iPS cells. Previously, we succeeded in converting miPS cells into cancer stem cells (CSCs) under the conditions of tumor microenvironment. Both stem cells and tumor cells are profoundly influenced by bi-directional communication with their respective microenvironment, which dictates cell fate determination and behavior. The microenvironment derived from iPS cells has not been well studied. In this paper, we have investigated the effects of secreted factors from Nanog-mouse iPS (miPS) cells on mouse Lewis lung cancer (LLC) cells that are found in the conditioned media. The results demonstrated that miPS cells secrete factors that can convert the epithelia phenotype of LLC cells to a mesenchymal phenotype, and that can promote tumorigenisity, migration and invasion. Furthermore, LLC cells that have been exposed to miPS conditioned medium became resistant to apoptosis. These various biological effects suggest that the miPS microenvironment contain factors that can promote an epithelial-mesenchymal transition (EMT) through an active Snail-MMP axis or by suppressing differentiation in LLC cells.
ACCESSION #
94750438

 

Related Articles

  • Hypothermia Stimulates Glioma Stem Spheres to Spontaneously Dedifferentiate Adjacent Non-stem Glioma Cells. Shi, Lei; Fei, Xifeng; Sun, Guan; Wang, Zhimin; Wan, Yi; Zeng, Yanjun; Guo, Jun // Cellular & Molecular Neurobiology;Mar2015, Vol. 35 Issue 2, p217 

    Current models of stem cell biology assume that glioma stem cells reside at the apices of hierarchies and differentiate into non-stem progeny in a unidirectional manner. However, here we found an opposite phenomenon that glioma stem spheres could induce adjacent non-stem glioma cells to...

  • Variation in Mesodermal and Hematopoietic Potential of Adult Skin-derived Induced Pluripotent Stem Cell Lines in Mice. Inoue, Tomoko; Kulkeaw, Kasem; Okayama, Satoko; Tani, Kenzaburo; Sugiyama, Daisuke // Stem Cell Reviews & Reports;Dec2011, Vol. 7 Issue 4, p958 

    Induced pluripotent stem cells (iPSCs) are a promising tool for regenerative medicine. Use of iPSC lines for future hematotherapy will require examination of their hematopoietic potential. Adult skin fibroblast somatic cells constitute a source of iPSCs that can be accessed clinically without...

  • JAK-STAT3 and somatic cell reprogramming. Yong Tang; Xiuchun (Cindy) Tian // JAK-STAT;Oct2013, Vol. 2 Issue 4, pe24935-1 

    Reprogramming somatic cells to pluripotency, especially by the induced pluripotent stem cell (iPSC) technology, has become widely used today to generate various types of stem cells for research and for regenerative medicine. However the mechanism(s) of reprogramming still need detailed...

  • Differentiation of mouse iPS cells into ameloblast-like cells in cultures using medium conditioned by epithelial cell rests of Malassez and gelatin-coated dishes. Yoshida, Koki; Sato, Jun; Takai, Rie; Uehara, Osamu; Kurashige, Yoshihito; Nishimura, Michiko; Chiba, Itsuo; Saitoh, Masato; Abiko, Yoshihiro // Medical Molecular Morphology;Sep2015, Vol. 48 Issue 3, p138 

    Induced pluripotent stem (iPS) cells are generated from adult cells and are potentially of great value in regenerative medicine. Recently, it was shown that iPS cells can differentiate into ameloblast-like cells in cultures using feeder cells. In the present study, we sought to induce...

  • Function of the IgM receptor. Fehervari, Zoltan // Nature Immunology;Apr2013, Vol. 14 Issue 4, p318 

    No abstract available.

  • Development of a simple, repeatable, and cost-effective extracellular matrix for long-term xeno-free and feeder-free self-renewal of human pluripotent stem cells. Pakzad, Mohammad; Ashtiani, Mohammad Kazemi; Mousavi-Gargari, Seyed Latif; Baharvand, Hossein // Histochemistry & Cell Biology;Dec2013, Vol. 140 Issue 6, p635 

    Given the potential importance of human pluripotent stem cells (hPSCs) in translational research and regenerative medicine, the aim of the present study was to develop a simple, safe, and cost-effective substrate to expand hPSCs. We report the development of an extracellular matrix (ECM),...

  • Vitamin C improves the quality of somatic cell reprogramming. Esteban, Miguel A; Pei, Duanqing // Nature Genetics;Apr2012, Vol. 44 Issue 4, p366 

    The reprogramming of somatic cells to a pluripotent state is prone to errors that could hamper the use of this technology. A new study now shows that vitamin C enhances the quality of somatic cell reprogramming in mice, highlighting the possibility that further manipulation of culture conditions...

  • Lung Cancer Stem Cells: Current Progress and Future Perspectives. Salama, Rasha; Tang, Joy; Gadgeel, Shirish M.; Ahmad, Aamir; Sarkar, Fazlul H. // Journal of Stem Cell Research & Therapy;Special2012, Vol. 2 Issue S, Special section p1 

    Cancer stem cells (CSCs) have attracted a lot of interest in the field of cancer research in recent years. Lung CSCs share many characteristics with the normal pluripotent stem cells, such as self-renewal and multi-potent abilities. Identification of normal adult lung stem cells and their...

  • Evidence for self-maintaining pluripotent murine stem cells in embryoid bodies. Attia, Wael; Abd El Aziz, Osama; Spitkovsky, Dimitry; Gaspar, John; Dröge, Peter; Suhr, Frank; Sabour, Davood; Winkler, Johannes; Meganathan, Kesavan; Jagtap, Smita; Khalil, Markus; Hescheler, Jürgen; Brockmeier, Konrad; Sachinidis, Agapios; Pfannkuche, Kurt // Stem Cell Reviews & Reports;Feb2014, Vol. 10 Issue 1, p1 

    Pluripotent stem cells have great potential for regenerative medicine; however, their clinical use is associated with a risk of tumor formation. We utilized pluripotent cells expressing green fluorescent protein and puromycin resistance under control of the Oct4 promoter to study the persistence...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics