A novel experimental hypoxia chamber for cell culture

Ruoxiang Wang; Fengshuo Jin; Hua Zhong
January 2014
American Journal of Cancer Research;2014, Vol. 4 Issue 1, p53
Academic Journal
Tissue hypoxia is a common pathophysiological process. Since 1990s, numerous studies have focused on investigating cellular adaptation to experimental hypoxia. A modular incubator chamber made of solid materials has frequently been used in the experiments that require hypoxic conditions. Here, we introduce a novel and inflatable chamber for hypoxia experiments. In experiments detecting hypoxia-induced accumulation of hypoxia-inducible factor 1α (HIF-1α) and hypoxia-induced expression of HIF-1-regulated genes, the new chamber yielded reproducible and comparable results as the modular incubator chamber did. The new chamber did not create inner chamber pressure during its use. Other properties of the new chamber were low-cost, easy to use, and leakage-free. Moreover, the size of the new chamber was adjustable, and the smaller one could be placed onto an inverted microscope for real-time studies. The successful examples of real-time studies included the real-time recording of GFP-HIF-1α fusion nuclear translocation and endothelial cell tubular formation.


Related Articles

  • The Tyrosine Phosphatase SHP-1 Regulates Hypoxia Inducible Factor-1α (HIF-1α) Protein Levels in Endothelial Cells under Hypoxia. Alig, Stefan K.; Stampnik, Yvonn; Pircher, Joachim; Rotter, Raffaela; Gaitzsch, Erik; Ribeiro, Andrea; Wörnle, Markus; Krötz, Florian; Mannell, Hanna // PLoS ONE;Mar2015, Vol. 10 Issue 3, p1 

    Introduction: The tyrosine phosphatase SHP-1 negatively influences endothelial function, such as VEGF signaling and reactive oxygen species (ROS) formation, and has been shown to influence angiogenesis during tissue ischemia. In ischemic tissues, hypoxia induced angiogenesis is crucial for...

  • BH4 domain of bcl-2 protein is required for its proangiogenic function under hypoxic condition. Gabellini, Chiara; De Luca, Teresa; Trisciuoglio, Daniela; Desideri, Marianna; Di Martile, Marta; Passeri, Daniela; Candiloro, Antonio; Biffoni, Mauro; Rizzo, Maria Giulia; Orlandi, Augusto; Del Bufalo, Donatella // Carcinogenesis;Nov2013, Vol. 34 Issue 11, p2558 

    Beyond its classical role as apoptosis inhibitor, bcl-2 protein promotes tumor angiogenesis and the removal of N-terminal bcl-2 homology (BH4) domain abrogates bcl-2-induced hypoxia-inducible factor 1 (HIF-1)-mediated vascular endothelial growth factor (VEGF) expression in hypoxic cancer cells....

  • A Novel Prolyl Hydroxylase Inhibitor Protects Against Cell Death After Hypoxia. Kontani, Satoru; Nagata, Eiichiro; Uesugi, Tsuyoshi; Moriya, Yusuke; Fujii, Natsuko; Miyata, Toshio; Takizawa, Shunya // Neurochemical Research;Dec2013, Vol. 38 Issue 12, p2588 

    Hypoxia-inducible factor 1 (HIF-1) is regulated by the oxygen-dependent hydroxylation of proline residues by prolyl hydroxylases (PHDs). We recently developed a novel PHD inhibitor, TM6008, that suppresses the activity of PHDs, inducing continuous HIF-1α activation. In this study, we...

  • Up-regulation of hypoxia inducible factor-1α by cobalt chloride correlates with proliferation and apoptosis in PC-2 cells.  // Journal of Experimental & Clinical Cancer Research (17569966);2012, Vol. 31 Issue 1, p28 

    The article presents a study investigating the variation of hypoxia-inducible factor-1α (HIF-1α) expression and the apoptosis effect of hypoxia stimulated by cobalt chloride in pancreatic cancer PC-2 cells. It is mentioned that hypoxia stimulated by cobalt chloride could effectively induce...

  • Adipose differentiation-related protein is not involved in hypoxia inducible factor-1-induced lipid accumulation under hypoxia. GUOMIN SHEN; NING NING; XINGSHENG ZHAO; XI LIU; GUANGYU WANG; TIANZHEN WANG; RAN ZHAO; CHAO YANG; DONGMEI WANG; PINGYUAN GONG; YAN SHEN; YONGJIAN SUN; XIAO ZHAO; YINJI JIN; WEIWEI YANG; YAN HE; LEI ZHANG; XIAOMING JIN; XIAOBO LI // Molecular Medicine Reports;2015, Vol. 12 Issue 6, p8055 

    Increasing evidence has showed that hypoxia inducible factor-1 (HIF1) has an important role in hypoxia-induced lipid accumulation, a common feature of solid tumors; however, its role remains to be fully elucidated. Adipose differentiation-related protein (ADRP), a structural protein of lipid...

  • A novel microtubule-modulating agent EM011 inhibits angiogenesis by repressing the HIF-1α axis and disrupting cell polarity and migration. Karna, Prasanthi; Rida, Padmashree C.G.; Turaga, Ravi Chakra; Gao, Jinmin; Gupta, Meenakshi; Fritz, Andreas; Werner, Erica; Yates, Clayton; Zhou, Jun; Aneja, Ritu // Carcinogenesis;Sep2012, Vol. 33 Issue 9, p1769 

    Endothelial tubular morphogenesis relies on an exquisite interplay of microtubule dynamics and actin remodeling to propel directed cell migration. Recently, the dynamicity and integrity of microtubules have been implicated in the trafficking and efficient translation of the mRNA for HIF-1α...

  • Pioglitazone inhibits HIF-1α-dependent angiogenesis in rats by paracrine and direct effects on endothelial cells. Dromparis, Peter; Sutendra, Gopinath; Paulin, Roxane; Proctor, Spencer; Michelakis, Evangelos; McMurtry, M. // Journal of Molecular Medicine;May2014, Vol. 92 Issue 5, p497 

    Pioglitazone was associated with increased hazard for surgical or percutaneous lower extremity revascularization in patients with diabetes in a large clinical trial, but this clinical finding has not been adequately explored in animal models. We hypothesized that pioglitazone would decrease...

  • Educational paper: pathogenesis of infantile haemangioma, an update 2014 (part I). Janmohamed, Sherief; Madern, Gerard; Laat, Peter; Oranje, Arnold // European Journal of Pediatrics;Jan2015, Vol. 174 Issue 1, p97 

    Infantile haemangioma (IH) is the most frequent childhood tumour. Although it is benign and self-limiting, severe complications can arise due to localisation and fast tumour growth. Management and therapy of IH has changed greatly after 2008 with propranolol. However, the pathogenesis remains...

  • Pre-B-cell colony enhancing factor (PBEF) increases endothelial permeability in hypoxia/re-oxygenation model. Wei Yang; Yuan Zeng; Bin Li; Jianliang Zhou; Yi Gong; Jianjun Xu; Xiao Dong // International Journal of Clinical & Experimental Medicine;2015, Vol. 8 Issue 6, p8842 

    Objective: This study aims to explore the relationship between PBEF and VEGF and p-MLC and the mechanism of PBEF increasing permeability of endothelial cells in hypoxia/re-oxygenation. Methods: Hypoxia/re-oxygenation model was established and PBEF siRNA was synthesized. According to the...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics