TITLE

Optimization and Characterization of Biosurfactant Producing Microbes and Expression of Biosurfact Producing Genes in Non Biosurfactant Producing Miocrobes

AUTHOR(S)
Pandey, Amit; Mishra, Mansi; Rai, Vineeta
PUB. DATE
March 2014
SOURCE
International Journal of Pharmaceutical Research & Allied Scienc;2014, Vol. 3 Issue 1, p47
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The present study is carried out by isolation and characterization of bio surfactant producing bacteria and expression of bio surfactant producing genes in non bio surfactant producing bacteria. The soil samples were collected from different regions of Lucknow and isolation as well as screening was done by using serial dilution and shake flask method respectively. According to Bergey's manual the 5 cultures were characterized they were- M.luteus, B.cereus, S.aureus, Neisseria and Alcaligens. The Bio surfactant activity was measured till 7 days and further optimization was done to get best carbon source, nitrogen source, pH and metal ions. Further the plasmids were isolated and restriction digestion, ligation were performed and the E.coli competent cells were used for transformation of bio surfactant producing genes in non bio surfactant producing bacteria.
ACCESSION #
94733742

 

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