TITLE

Characterization of the glutathione S-transferase gene family through ESTs and expression analyses within common and pigmented cultivars of Citrus sinensis (L.) Osbeck

AUTHOR(S)
Licciardello, Concetta; D' Agostino, Nunzio; Traini, Alessandra; Recupero, Giuseppe Reforgiato; Frusciante, Luigi; Chiusano, Maria Luisa
PUB. DATE
February 2014
SOURCE
BMC Plant Biology;2014, Vol. 14 Issue 1, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background Glutathione S-transferases (GSTs) represent a ubiquitous gene family encoding detoxification enzymes able to recognize reactive electrophilic xenobiotic molecules as well as compounds of endogenous origin. Anthocyanin pigments require GSTs for their transport into the vacuole since their cytoplasmic retention is toxic to the cell. Anthocyanin accumulation in Citrus sinensis (L.) Osbeck fruit flesh determines different phenotypes affecting the typical pigmentation of Sicilian blood oranges. In this paper we describe: i) the characterization of the GST gene family in C. sinensis through a systematic EST analysis; ii) the validation of the EST assembly by exploiting the genome sequences of C. sinensis and C. clementina and their genome annotations; iii) GST gene expression profiling in six tissues/organs and in two different sweet orange cultivars, Cadenera (common) and Moro (pigmented). Results We identified 61 GST transcripts, described the full- or partial-length nature of the sequences and assigned to each sequence the GST class membership exploiting a comparative approach and the classification scheme proposed for plant species. A total of 23 full-length sequences were defined. Fifty-four of the 61 transcripts were successful aligned to the C. sinensis and C. clementina genomes. Tissue specific expression profiling demonstrated that the expression of some GST transcripts was 'tissue-affected' and cultivar specific. A comparative analysis of C. sinensis GSTs with those from other plant species was also considered. Data from the current analysis are accessible at http://biosrv.cab.unina.it/citrusGST/, with the aim to provide a reference resource for C. sinensis GSTs. Conclusions This study aimed at the characterization of the GST gene family in C. sinensis. Based on expression patterns from two different cultivars and on sequence-comparative analyses, we also highlighted that two sequences, a Phi class GST and a Mapeg class GST, could be involved in the conjugation of anthocyanin pigments and in their transport into the vacuole, specifically in fruit flesh of the pigmented cultivar.
ACCESSION #
94445146

 

Related Articles

  • Identification and characterization of 27 conserved microRNAs in citrus. Changnian Song; Jinggui Fang; Xiaoying Li; Hong Liu; Chao, C. Thomas // Planta;Sep2009, Vol. 230 Issue 4, p671 

    MicroRNAs (miRNAs) are a class of non-protein-coding small RNAs. Considering the conservation of many miRNA genes in different plant genomes, the identification of miRNAs from non-model organisms is both practicable and instrumental in addressing miRNA-guided gene regulation. Citrus is an...

  • Genetic linkage maps of two apricot cultivars (Prunus armeniaca L.) compared with the almond Texas × peach Earlygold reference map for Prunus. Lambert, P.; Hagen, L. S.; Arus, P.; Audergon, J. M. // Theoretical & Applied Genetics;Apr2004, Vol. 108 Issue 6, p1120 

    Several genetic linkage maps have been published in recent years on different Prunus species suggesting a high level of resemblance among the genomes of these species. One of these maps (Joobeur et al., Theor Appl Genet 97:1034–1041 [(1998); Aranzana et al., Theor Appl Genet...

  • Survey and molecular detection of two citrus viroids affecting commercial citrus orchards in the Northern part of Sudan. Abubaker, Mohamed Yousif Adam; Elhassan, Siddig Mohamed // Agriculture & Biology Journal of North America;2010, Vol. 1 Issue 5, p930 

    Two extensive surveys and laboratory work were conducted to determine the occurrence of citrus exocortis viroid (CEVd) and hop stunt viroid (HSVd), the causal agent of cachexia (CVd-IIb) disease in the main citrus growing areas in three states in the northern part of Sudan, viz, Northern, River...

  • A reference genetic map of C. clementina hort. ex Tan.; citrus evolution inferences from comparative mapping. Ollitrault, Patrick; Terol, Javier; Chunxian Chen; Federici, Claire T.; Lotfy, Samia; Hippolyte, Isabelle; Ollitrault, Fr�d�rique; B�rard, Aur�lie; Chauveau, Aur�lie; Cuenca, Jose; Costantino, Gilles; Kacar, Yildiz; Mu, Lisa; Garcia-Lor, Andres; Froelicher, Yann; Aleza, Pablo; Boland, Anne; Billot, Claire; Navarro, Luis; Luro, Fran�ois // BMC Genomics;2012, Vol. 13 Issue 1, p593 

    Background: Most modern citrus cultivars have an interspecific origin. As a foundational step towards deciphering the interspecific genome structures, a reference whole genome sequence was produced by the International Citrus Genome Consortium from a haploid derived from Clementine mandarin. The...

  • Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp. aurantifolii. Moreira, Leandro M.; Almeida, Jr., Nalvo F.; Potnis, Neha; Digiampietri, Luciano A.; Adi, Said S.; Bortolossi, Julio C.; da Silva, Ana C.; da Silva, Aline M.; de Moraes, Fabrício E.; de Oliveira, Julio C.; de Souza, Robson F.; Facincani, Agda P.; Ferraz, André L.; Ferro, Maria I.; Furlan, Luiz R.; Gimenez, Daniele F.; Jones, Jeffrey B.; Kitajima, Elliot W.; Laia, Marcelo L.; Leite, Jr., Rui P. // BMC Genomics;2010, Vol. 11, p238 

    Background: Citrus canker is a disease that has severe economic impact on the citrus industry worldwide. There are three types of canker, called A, B, and C. The three types have different phenotypes and affect different citrus species. The causative agent for type A is Xanthomonas citri subsp....

  • Citrus genomics. Gmitter, Fred; Chen, Chunxian; Machado, Marcos; Souza, Alessandra; Ollitrault, Patrick; Froehlicher, Yann; Shimizu, Tokurou // Tree Genetics & Genomes;Jun2012, Vol. 8 Issue 3, p611 

    Citrus fruits (sweet orange, mandarin, pummelo, grapefruit, lemon, lime and assorted hybrids) are among the most widely grown and economically important fruit tree crops in the world. As fresh fruit, they are an important and nutrient dense food source for human diets; as processed juice...

  • A genealogy of the citrus family. Velasco, Riccardo; Licciardello, Concetta // Nature Biotechnology;Jul2014, Vol. 32 Issue 7, p640 

    The article focuses on the study of genetic relationship among several citrus fruits including clementines, tangerines and grapefruits and also mention the genomic sequences of the fruits. Topics include phylogenetic history of the fruits, identification of superior varieties of fruits resistant...

  • Further Arguments. Sarai, Sarah // Minnesota Review (Minnesota Review);Spring2007, Issue 68, p5 

    Presents the poem "Further Arguments," by Sarah Sarai. First Line: If there is a god you must sculpt my bellied likeness then; Last Line: of us is what I am calling god.

  • Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus. María Zanek; Carina Reyes; Magdalena Cervera; Eduardo Peña; Karelia Velázquez; Norma Costa; Maria Plata; Oscar Grau; Leandro Peña; María García // Plant Cell Reports;Jan2008, Vol. 27 Issue 1, p57 

    Abstract  Citrus psorosis is a serious viral disease affecting citrus trees in many countries. Its causal agent is Citrus psorosis virus (CPsV), the type member of genus Ophiovirus. CPsV infects most important citrus varieties, including oranges, mandarins and grapefruits, as...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics