夏 嘉 勇; 詹 雅 慧; 張 毅 偉; 陳 瑋 芸; 曾 素 香
April 2013
Taiwanese Journal of Agricultural Chemistry & Food Science;Apr2013, Vol. 51 Issue 2, p51
Academic Journal
A high performance liquid chromatographic (HPLC) method was developed and validated for simultaneous determination of β-carotene, lutein, lycopene and zeaxanthin in tablet and capsule products. Samples were dispersed in water and extracted with ethyl acetate before analysing with HPLC. The HPLC was performed on a YMC Carotenoids C30 (4.6 × 250 mm ) column and a gradient mobile phase profile, and the flow rate was 1 mL/min. The chromatography was detected at 450 nm for β-carotene, lutein and zeaxanthin, and 470 nm for lycopene. The specificity of the method was demonstrated by the retention characteristics, HPLC spectra and by comparing the peak purity with the standard of each compound. The calibration curves ( β-carotene 0.1-2 μg/mL , lutein 0.05-1 μg/mL , lycopene 5-100 μg/mL and zeaxanthin 0.5-10 μg/mL ) plotted with five concentrations of each compound were linear with a determination coefficient R2 > 0.9960. It was found to be satisfactory in terms of accuracy (recovery between 92.3% and 112.2% ) and precision (intra-day and inter-day variability between 0.17-4.74% and 0.13-5.72% , respectively). The limits of quantification (LOQ) were 10, 50, 500 and 50 μg/g for β-carotene, lutein, lycopene and zeaxanthin, respectively. The HPLC method was applied to SRM3280, a standard reference material for Multivitamin/Multielement Tablets, and the results all met the specification of SRM 3280. Four samples purchased from the market were analyzed by this method to ensure the applicability. The results showed that the consistency is between 84-137%.


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