TITLE

Effects of downregulation of PRDM14 expression on cell proliferation, cell cycle and invasion ability in esophageal quamous cell carcinoma EC9706 cells

AUTHOR(S)
LI Shengke; TIAN Fang; LIU Hongtao; JIANG Zuguang; YUE Wenbin; LIU Yuefen; ZHOU Honglin
PUB. DATE
February 2014
SOURCE
Journal of Huazhong Normal University;Feb2014, Vol. 48 Issue 1, p80
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
In situ hybridization and immunohistochemistry methods were utilized to investigate the expressions of PRDM14 mRNA transcription level and protein level in 89 cases of esophageal squamous cell carcinoma (ESCC) tissues and paired normal esophageal epithelial tissues and effects of its expression downregulation on cell proliferation, cell cycle and invasion ability were analyzed in EC9706 cells. The results demonstrated that expressions of PRDM14 mRNA transcription level and protein level in ESCC tissues were higher than that in normal esophageal epithelial tissues, and there was a significant difference (P<0. 05). PRDM14 siRNA downregulated the expression of PRDM14 protein in EC9706 cells, and its downregulation obviously suppressed cell proliferation, altered cell cycle distribution and reduced invasion ability. In addition, downregulation of PRDM14expression evoked the decreases of cyclin D1, cdk2, MMP-2 and MMP-9 protein levels as well as increased the expressions of p21 and E-cadherin proteins. These data above suggest that PRDM14 may play a pivotal role in the occurrence and development of ESCC, and thus PRDM14 inhibition may be a novel and promising strategy for molecular targeted therapy of the patients with ESCC.
ACCESSION #
93743314

 

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