TITLE

Bacterial polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenases in the sediments from the Pearl River estuary, China

AUTHOR(S)
Wu, Peng; Wang, You-Shao; Sun, Fu-Lin; Wu, Mei-Lin; Peng, Ya-lan
PUB. DATE
January 2014
SOURCE
Applied Microbiology & Biotechnology;Jan2014, Vol. 98 Issue 2, p875
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Bacterial community compositions were characterized using denaturing gradient gel electrophoresis analysis of bacterial 16S rRNA gene in the sediments of the Pearl River estuary. Sequencing analyses of the excised bands indicated that Gram-negative bacteria, especially Gammaproteobacteria, were dominant in the Pearl River estuary. The diversity of polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase (PAH-RHD) gene in this estuary was then assessed by clone library analysis. The phylogenetic analyses showed that all PAH-RHD gene sequences of Gram-negative bacteria (PAH-RHD) were closely related to the nagAc gene described for Ralstonia sp. U2 or nahAc gene for Pseudomonas sp. 9816-4, while the PAH-RHD gene sequences of Gram-positive bacteria (PAH-RHD) at sampling site A1 showed high sequence similarity to the nidA gene from Mycobacterium species. Meanwhile, molecular diversity of the two functional genes was higher at the upstream of this region, while lower at the downstream. Redundancy analysis indicated that environmental factors, such as NH-N, ∑PAHs, pH, SiO-Si, and water depth, affected the distribution of the PAH-RHD gene in the Pearl River estuary.
ACCESSION #
93646528

 

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