An investigation into IgE-facilitated allergen recognition and presentation by human dendritic cells

Sharquie, Inas K.; Al-Ghouleh, Abeer; Fitton, Patricia; Clark, Mike R.; Armour, Kathryn L.; Sewell, Herb F.; Shakib, Farouk; Ghaemmaghami, Amir M.
January 2013
BMC Immunology;2013, Vol. 14 Issue 1, p1
Academic Journal
Background Allergen recognition by dendritic cells (DCs) is a key event in the allergic cascade leading to production of IgE antibodies. C-type lectins, such as the mannose receptor and DC-SIGN, were recently shown to play an important role in the uptake of the house dust mite glycoallergen Der p 1 by DCs. In addition to mannose receptor (MR) and DC-SIGN the high and low affinity IgE receptors, namely FcεRI and FcεRII (CD23), respectively, have been shown to be involved in allergen uptake and presentation by DCs. Objectives This study aims at understanding the extent to which IgE- and IgG-facilitated Der p 1 uptake by DCs influence T cell polarisation and in particular potential bias in favour of Th2. We have addressed this issue by using two chimaeric monoclonal antibodies produced in our laboratory and directed against a previously defined epitope on Der p 1, namely human IgE 2C7 and IgG1 2C7. Results Flow cytometry was used to establish the expression patterns of IgE (FcεRI and FcεRII) and IgG (FcγRI) receptors in relation to MR on DCs. The impact of FcεRI, FcεRII, FcγRI and mannose receptor mediated allergen uptake on Th1/Th2 cell differentiation was investigated using DC/T cell co-culture experiments. Myeloid DCs showed high levels of FcεRI and FcγRI expression, but low levels of CD23 and MR, and this has therefore enabled us to assess the role of IgE and IgG-facilitated allergen presentation in T cell polarisation with minimal interference by CD23 and MR. Our data demonstrate that DCs that have taken up Der p 1 via surface IgE support a Th2 response. However, no such effect was demonstrable via surface IgG. Conclusions IgE bound to its high affinity receptor plays an important role in Der p 1 uptake and processing by peripheral blood DCs and in Th2 polarisation of T cells.


Related Articles

  • T-cell responses: DCs get KITted out. Bird, Lucy // Nature Reviews Immunology;Jun2008, Vol. 8 Issue 6, p406 

    The article focuses on the research which explains how the allergen housedust mite (HDM) and the mucosal adjuvant cholera toxin act on dendritic cells to promote T helper cell differentiation. It also shows how antigens or allergens influence (DCs) to guide a particular pathway involving the...

  • House dust mite allergen induces asthma via Toll-like receptor 4 triggering of airway structural cells. Hammad, Hamida; Chieppa, Marcello; Perros, Frederic; Willart, Monique A.; Germain, Ronald N.; Lambrecht, Bart N. // Nature Medicine;Apr2009, Vol. 15 Issue 4, p410 

    Barrier epithelial cells and airway dendritic cells (DCs) make up the first line of defense against inhaled substances such as house dust mite (HDM) allergen and endotoxin (lipopolysaccharide, LPS). We hypothesized that these cells need to communicate with each other to cause allergic disease....

  • House dust mite allergens mediate the activation of c-kit in dendritic cells via Toll-like receptor 2. WEI WU; CHUN-XIA WANG; HUI CHEN; JING ZHOU; JIN-ZHAO ZHANG; LIN GAO; HONG-YAN ZHOU // Molecular Medicine Reports;2015, Vol. 12 Issue 4, p5307 

    Several studies have demonstrated that the c-kit proto-oncogene and its ligand, stem cell factor, are important in the development of asthma. House dust mite (HDM; Dermatophagoides pteronyssinus) allergens are a major trigger in the development and exacerbation of asthma. HDM allergens can...

  • Resident CD11b+Ly6C- Lung Dendritic Cells Are Responsible for Allergic Airway Sensitization to House Dust Mite in Mice. Mesnil, Claire; Sabatel, Catherine M.; Marichal, Thomas; Toussaint, Marie; Cataldo, Didier; Drion, Pierre- Vincent; Lekeux, Pierre; Bureau, Fabrice; Desmet, Christophe J. // PLoS ONE;Dec2012, Vol. 7 Issue 12, p1 

    Conventional dendritic cells (DCs) are considered to be the prime initiators of airway allergy. Yet, it remains unclear whether specific DC subsets are preferentially involved in allergic airway sensitization. Here, we systematically assessed the respective pro-allergic potential of individually...

  • Immuno-modulatory activity of Ganoderma lucidum-derived polysacharide on human monocytoid dendritic cells pulsed with Der p 1 allergen. Rong-Hwa Jan; Teng-Yi Lin; Ya-Chun Hsu; Shiuh-Sheng Lee; Shih-Yen Lo; Mingi Chang; Li-Kuang Chen; Yu-Li Lin // BMC Immunology;2011, Vol. 12 Issue 1, p31 

    Background: Ganoderma lucidum-derived polysaccharide (PS-G) can rapidly and effectively promote the activation and maturation of immature dendritic cells (DCs), suggesting that PS-G possesses the capacity to regulate immune responses. This study aimed to clarify the immunologic effect of PS-G on...

  • Patch test may help understanding of dust mite. Nemec, John // Dermatology Times;Jun2002, Vol. 23 Issue 6, p42 

    Focuses on the benefit of standardized patch test allergen in diagnosing allergens to house dust mites in the U.S. Improvement of the diagnosis of allergies; Availability of allergen patch test kit; Investigation of the effect of dust mite in atopic dermatitis.

  • Dust mites' dirty dealings with dendritic cells. Sheppard, Dean // Nature Medicine;May2008, Vol. 14 Issue 5, p487 

    The article examines the multicellular pathway in which allergens act directly on dendritic cells to drive differentiation of both T helper type 2 cells and T lymphocyte. Krishnamoorthy and colleagues used recognition receptors to recognize common components of viruses and bacteria in the cells...

  • Mite allergen decreases DC-SIGN expression and modulates human dendritic cell differentiation and function in allergic asthma. Huang, H.-J.; Lin, Y.-L.; Liu, C.-F.; Kao, H.-F.; Wang, J.-Y. // Mucosal Immunology (1933-0219);Sep2011, Vol. 4 Issue 5, p519 

    House dust mites (HDMs) induce allergic asthma in sensitized individuals, although how HDMs activate immature mucosal dendritic cells (DCs) to render the T helper cell type 2 (Th2)-mediated immune response is unclear. In this study, our results showed a significant calcium-dependent lectin...

  • Defining a role for ambient TLR ligand exposures in the genesis and prevention of allergic diseases. Tse, Kevin; Horner, Anthony // Seminars in Immunopathology;2008, Vol. 30 Issue 1, p53 

    Environmental variables responsible for the increasing allergic disease burden observed in developed countries over the last century have yet to be adequately characterized. Meta-analyses of epidemiological studies presented in the first half of this paper demonstrate a correlation between...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics