TITLE

Anti-senescence efficacy of radio-electric asymmetric conveyer technology

AUTHOR(S)
Maioli, Margherita; Rinaldi, Salvatore; Santaniello, Sara; Castagna, Alessandro; Pigliaru, Gianfranco; Delitala, Alessandro; Lotti Margotti, Matteo; Bagella, Luigi; Fontani, Vania; Ventura, Carlo
PUB. DATE
February 2014
SOURCE
Age;Feb2014, Vol. 36 Issue 1, p9
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Recent evidence suggests that ageing-related diseases could result in an accelerated loss of self-renewal capability of adult stem cells, normally involved in replacing damaged cellular elements. In previous works, we highlighted that a specific treatment, named tissue optimization-regenerative (TO-RGN), of radio-electric asymmetric conveyer (REAC) technology, influenced gene expression profiles controlling stem cell differentiation and pluripotency of human skin-derived fibroblasts in vitro. The purpose of the present work was to verify whether TO-RGN may also be effective in counteracting the expression of the senescence marker beta-galactosidase and of senescence-associated gene expression patterning, engaged during prolonged culture of human adipose-derived stem cells (hADSCs). Following TO-RGN exposure, we observed a significant downregulation in beta-galactosidase staining and in the expression of the senescence mediator genes p16INK4, ARF, p53, and p21. Moreover, differently formed untreated cells, TO-RGN-exposed hADSCs maintained their typical fibroblast-like morphology and exhibited a multilineage potential even at late passages, as shown by the remarkable preservation of commitment to osteogenic, adipogenic, chondrogenic, and vasculogenic fates, both at morphologic and gene expression levels. In conclusion, our study highlights a positive effect of TO-RGN in counteracting degenerative senescence processes in vitro.
ACCESSION #
93447577

 

Related Articles

  • Lineage conversion induced by pluripotency factors involves transient passage through an iPSC stage. Bar-Nur, Ori; Verheul, Cassandra; Sommer, Andreia G; Brumbaugh, Justin; Schwarz, Benjamin A; Lipchina, Inna; Huebner, Aaron J; Mostoslavsky, Gustavo; Hochedlinger, Konrad // Nature Biotechnology;Jul2015, Vol. 33 Issue 7, p761 

    Brief expression of pluripotency-associated factors such as Oct4, Klf4, Sox2 and c-Myc (OKSM), in combination with differentiation-inducing signals, has been reported to trigger transdifferentiation of fibroblasts into other cell types. Here we show that OKSM expression in mouse fibroblasts...

  • The piggyBac Transposon-Mediated Expression of SV40 T Antigen Efficiently Immortalizes Mouse Embryonic Fibroblasts (MEFs). Wang, Ning; Zhang, Wenwen; Cui, Jing; Zhang, Hongmei; Chen, Xiang; Li, Ruidong; Wu, Ningning; Chen, Xian; Wen, Sheng; Zhang, Junhui; Yin, Liangjun; Deng, Fang; Liao, Zhan; Zhang, Zhonglin; Zhang, Qian; Yan, Zhengjian; Liu, Wei; Ye, Jixing; Deng, Youlin; Wang, Zhongliang // PLoS ONE;May2014, Vol. 9 Issue 5, p1 

    Mouse embryonic fibroblasts (MEFs) are mesenchymal stem cell (MSC)-like multipotent progenitor cells and can undergo self-renewal and differentiate into to multiple lineages, including bone, cartilage and adipose. Primary MEFs have limited life span in culture, which thus hampers MEFs’...

  • Genetically Matched Human iPS Cells Reveal that Propensity for Cartilage and Bone Differentiation Differs with Clones, not Cell Type of Origin. Nasu, Akira; Ikeya, Makoto; Yamamoto, Takuya; Watanabe, Akira; Jin, Yonghui; Matsumoto, Yoshihisa; Hayakawa, Kazuo; Amano, Naoki; Sato, Shingo; Osafune, Kenji; Aoyama, Tomoki; Nakamura, Takashi; Kato, Tomohisa; Toguchida, Junya // PLoS ONE;Jan2013, Vol. 8 Issue 1, Special section p1 

    Background: For regenerative therapy using induced pluripotent stem cell (iPSC) technology, cell type of origin to be reprogrammed should be chosen based on accessibility and reprogramming efficiency. Some studies report that iPSCs exhibited a preference for differentiation into their original...

  • Human skin-derived keratinocytes and fibroblasts co-cultured on 3D poly ε-caprolactone scaffold support in vitro HSC differentiation into T-lineage committed cells. Palamaro, Loredana; Guarino, Vincenzo; Scalia, Giulia; Antonini, Dario; De Falco, Luigia; Bianchino, Gabriella; Fusco, Anna; Romano, Rosa; Grieco, Vitina; Missero, Caterina; Del Vecchio, Luigi; Ambrosio, Luigi; Pignata, Claudio // International Immunology;Dec2013, Vol. 25 Issue 12, p703 

    An artificial scaffold with skin-derived cells supports T-cell differentiation from HSCs.In humans, the thymus is the primary lymphoid organ able to support the development of T cells through its three-dimensional (3D) organization of the thymic stromal cells. Since a remarkable number of...

  • Identification of Appropriate Reference Genes for Human Mesenchymal Cells during Expansion and Differentiation. Amable, Paola Romina; Teixeira, Marcus Vinicius Telles; Carias, Rosana Bizon Vieira; Granjeiro, José Mauro; Borojevic, Radovan // PLoS ONE;Sep2013, Vol. 8 Issue 9, p1 

    Background: Quantitative real time polymerase chain reaction (qPCR) is an extremely powerful technique for monitoring gene expression. The quantity of the messenger ribonucleic acids (mRNA) of interest should be normalized using a reference gene, in order to avoid unreliable results originated...

  • A Role for Topographic Cues in the Organization of Collagenous Matrix by Corneal Fibroblasts and Stem Cells. Karamichos, Dimitrios; Funderburgh, Martha L.; Hutcheon, Audrey E. K.; Zieske, James D.; Du, Yiqin; Wu, Jian; Funderburgh, James L. // PLoS ONE;Jan2014, Vol. 9 Issue 1, p1 

    Human corneal fibroblasts (HCF) and corneal stromal stem cells (CSSC) each secrete and organize a thick stroma-like extracellular matrix in response to different substrata, but neither cell type organizes matrix on tissue-culture polystyrene. This study compared cell differentiation and...

  • A Combination of Culture Conditions and Gene Expression Analysis Can Be Used to Investigate and Predict hES Cell Differentiation Potential towards Male Gonadal Cells. Kjartansdóttir, Kristín Rós; Reda, Ahmed; Panula, Sarita; Day, Kelly; Hultenby, Kjell; Söder, Olle; Hovatta, Outi; Stukenborg, Jan-Bernd // PLoS ONE;12/2/2015, Vol. 10 Issue 12, p1 

    Human embryonic stem cell differentiation towards various cell types belonging to ecto-, endo- and mesodermal cell lineages has been demonstrated, with high efficiency rates using standardized differentiation protocols. However, germ cell differentiation from human embryonic stem cells has been...

  • The Effect of Human and Mouse Fibroblast Feeder Cells on Cardiac Differentiation of Human Pluripotent Stem Cells. Pekkanen-Mattila, Mari; Ojala, Marisa; Kerkelä, Erja; Rajala, Kristiina; Skottman, Heli; Aalto-Setälä, Katriina // Stem Cells International;2012, p1 

    Mouse embryonic fibroblasts (MEFs) and human foreskin fibroblasts (hFFs) are commonly used as feeder cells to maintain the pluripotent state of stem cells. The aim of the present study was to evaluate the effect of MEF and hFF feeders on the cardiac differentiation. Two human embryonic and two...

  • Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Aasen, Trond; Raya, Angel; Barrero, Maria J; Garreta, Elena; Consiglio, Antonella; Gonzalez, Federico; Vassena, Rita; Bilić, Josipa; Pekarik, Vladimir; Tiscornia, Gustavo; Edel, Michael; Boué, Stéphanie; Belmonte, Juan Carlos Izpisúa // Nature Biotechnology;Nov2008, Vol. 26 Issue 11, p1276 

    The utility of induced pluripotent stem (iPS) cells for investigating the molecular logic of pluripotency and for eventual clinical application is limited by the low efficiency of current methods for reprogramming. Here we show that reprogramming of juvenile human primary keratinocytes by...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics