TITLE

MicroRNA-339 and microRNA-556 regulate Klotho expression in vitro

AUTHOR(S)
Mehi, Stephen; Maltare, Astha; Abraham, Carmela; King, Gwendalyn
PUB. DATE
February 2014
SOURCE
Age;Feb2014, Vol. 36 Issue 1, p141
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Klotho is an anti-aging protein with direct effects on life-span in mice. Klotho functions to regulate pathways classically associated with longevity including insulin/IGF1 and Wnt signaling. Decreased Klotho protein expression is observed throughout the body during the normal aging process. While increased methylation of the Klotho promoter is reported, other epigenetic mechanisms could contribute to age-related downregulation of Klotho expression, including microRNA-mediated regulation. Following in silico identification of potential microRNA binding sites within the Klotho 3′ untranslated region, reporter assays reveal regulation by microRNA-339, microRNA-556, and, to a lesser extent, microRNA-10 and microRNA-199. MicroRNA-339 and microRNA-556 were further found to directly decrease Klotho protein expression indicating that, if upregulated in aging tissue, these microRNA could play a role in age-related downregulation of Klotho messenger RNA. These microRNAs are differentially regulated in cancer cells compared to normal cells and may imply a role for microRNA-mediated regulation of Klotho in cancer.
ACCESSION #
93447563

 

Related Articles

  • Identification of a New Target of miR-16, Vacuolar Protein Sorting 4a. Adhikari, Neeta; Guan, Weihua; Capaldo, Brian; Mackey, Aaron J.; Carlson, Marjorie; Ramakrishnan, Sundaram; Walek, Dinesha; Gupta, Manu; Mitchell, Adam; Eckman, Peter; John, Ranjit; Ashley, Euan; Barton, Paul J.; Hall, Jennifer L. // PLoS ONE;Jul2014, Vol. 9 Issue 7, p1 

    Rationale: The rationale was to utilize a bioinformatics approach to identify miRNA binding sites in genes with single nucleotide mutations (SNPs) to discover pathways in heart failure (HF). Objective: The objective was to focus on the genes containing miRNA binding sites with miRNAs that were...

  • Dual role of FoxA1 in androgen receptor binding to chromatin, androgen signalling and prostate cancer. Sahu, Biswajyoti; Laakso, Marko; Ovaska, Kristian; Mirtti, Tuomas; Lundin, Johan; Rannikko, Antti; Sankila, Anna; Turunen, Juha-Pekka; Lundin, Mikael; Konsti, Juho; Vesterinen, Tiina; Nordling, Stig; Kallioniemi, Olli; Hautaniemi, Sampsa; Jänne, Olli A // EMBO Journal;10/5/2011, Vol. 30 Issue 19, p3962 

    High androgen receptor (AR) level in primary tumour predicts increased prostate cancer-specific mortality. However, the mechanisms that regulate AR function in prostate cancer are poorly known. We report here a new paradigm for the forkhead protein FoxA1 action in androgen signalling. Besides...

  • The role of the coreceptor Orco in insect olfactory transduction. Stengl, Monika; Funk, Nico // Journal of Comparative Physiology A: Neuroethology, Sensory, Neu;Nov2013, Vol. 199 Issue 11, p897 

    Insects sense odorants with specialized odorant receptors (ORs). Each antennal olfactory receptor neuron expresses one OR with an odorant binding site together with a conserved coreceptor called Orco which does not bind odorants. Orco is necessary for localization of ORs to dendritic membranes...

  • A New Exhaustive Method and Strategy for Finding Motifs in ChIP-Enriched Regions. Jia, Caiyan; Carson, Matthew B.; Wang, Yang; Lin, Youfang; Lu, Hui // PLoS ONE;Jan2014, Vol. 9 Issue 1, p1 

    ChIP-seq, which combines chromatin immunoprecipitation (ChIP) with next-generation parallel sequencing, allows for the genome-wide identification of protein-DNA interactions. This technology poses new challenges for the development of novel motif-finding algorithms and methods for determining...

  • Human SNF2L Gene Is Regulated Constitutively and Inducibly in Neural Cells via a cAMP-Response Element. Yu Xia; Laicheng Wang; Chunyan Ma; Yaoqin Gong; Yueran Zhao // Yonsei Medical Journal;May2013, Vol. 54 Issue 3, p772 

    Purpose: SNF2L belongs to Imitation Switch family and plays an essential role in neural tissues and gonads. In our previous studies, we have demonstrated that the basal transcription of human SNF2L gene is regulated by two cis-elements, cAMP response element (CRE)- and Sp1-binding sites. Recent...

  • Both MicroRNA-155 and Virus-Encoded MiR-155 Ortholog Regulate TLR3 Expression. Hu, Xuming; Ye, Jianqiang; Qin, Aijian; Zou, Haitao; Shao, Hongxia; Qian, Kun // PLoS ONE;May2015, Vol. 10 Issue 5, p1 

    MicroRNA-155 (miR-155) has been as an important controller of TLR3 signalling. However, the interactions between miR-155 and TLR3 are poorly understood. Here, we focused on the regulation of the relationship between miR-155 and TLR3. Sequence analyses and firefly luciferase reporter assay...

  • An insertion/deletion polymorphism at the microRNA-122 binding site in the interleukin-1α 3'-untranslated region is associated with a risk for osteoarthritis. FAN YANG; ANFENG HU; DEWEI ZHAO; LIN GUO; LEI YANG; BENJIE WANG; FENGDE TIAN; BAOYI LIU; SHIBO HUANG; HUI XIE // Molecular Medicine Reports;2015, Vol. 12 Issue 4, p6199 

    Polymorphisms located at microRNA (miRNA) binding sites may affect the expression of genes. The present study aimed to identify the association between an insertion/ deletion (Ins/Del) polymorphism (rs3783553) in the 3'-untranslated region (3'-UTR) of interleukin-1a (IL-1A) and the risk for...

  • Signal Transducer and Activator of Transcription–3 Induces MicroRNA-155 Expression in Chronic Lymphocytic Leukemia. Li, Ping; Grgurevic, Srdana; Liu, Zhiming; Harris, David; Rozovski, Uri; Calin, George A.; Keating, Michael J.; Estrov, Zeev // PLoS ONE;Jun2013, Vol. 8 Issue 6, p1 

    MicroRNA (miR) abnormalities play a key role in the pathogenesis of chronic lymphocytic leukemia (CLL). High levels of miR-155 have been detected in human neoplasms, and overexpression of miR-155 has been found to induce lymphoma in mice. High levels of miR-155 were detected in CLL cells and...

  • Differential Ligand Binding Affinities of Human Estrogen Receptor-α Isoforms. Lin, Amanda H. Y.; Li, Rachel W. S.; Ho, Eva Y. W.; Leung, George P. H.; Leung, Susan W. S.; Vanhoutte, Paul M.; Man, Ricky Y. K. // PLoS ONE;Apr2013, Vol. 8 Issue 4, p1 

    Rapid non-genomic effects of 17β-estradiol are elicited by the activation of different estrogen receptor-α isoforms. Presence of surface binding sites for estrogen have been identified in cells transfected with full-length estrogen receptor-α66 (ER66) and the truncated isoforms,...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics