TITLE

THE ROLE OF GHRELIN AGAINST ACUTE CARBON TETRACHLORIDE HEPATOTOXICITY IN RATS

AUTHOR(S)
Şahin, Sabiha; Alataş, Özkan
PUB. DATE
September 2013
SOURCE
Nobel Medicus Journal;Sep-Dec2013, Vol. 9 Issue 3, p43
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Objective: Due to its anti-inflammatory and antioxidant effects, the peptide ghrelin has a protective effect in a variety of tissues. Antioxidant properties of ghrelin have been recently reported in various types of oxidative stress. Therefore, we designed this study to explore the antioxidative effects of ghrelin in acute liver injury induced by carbon tetrachloride (CCl4) in rats. Material and Method: In this experimental study, 24 Spraque-Dawley genus albino rats were equally divided into three groups as follows: control, CCU and CCl4+ghrelin. 4 ml/kg olive oil was administered intraperitoneally (i.p.) to the control group, 4 ml/kg CCl4 (1:1 dissolved in olive oil) was administered i.p. to the animals in other two groups. After three and six hours, 80 mcg/kg ghrelin was administered i.p. to the CCl4+ghrelin group. Twenty four hours after administrating CCU, all of the rats were sacrificed. Biochemical assessments were performed using serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA), tissue MDA, myeloperoxidase (MPO) and nitric oxide (NO) levels. Histopathological assesments were performed using hematoxilen & eosine staining in light microscope. Results: Serum AST, ALT, MDA and tissue MDA, MPO levels were all increased in rats exposed to CCU. These values were significantly decreased in the group subsequently treated with ghrelin. Histopathological data showed a significant decrease in congestion, polymorphonuclear leukocytes, mononuclear leukocytes, vacuolar degeneration of hepatocytes and hepatocellular necrosis in rats treated with ghrelin. Conclusion: No effective therapies are currently available for patients with acute liver injury. Our study indicates that ghrelin protected rats from acute liver injury by reducing oxidative stress and inflammation.
ACCESSION #
93443851

 

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