Isolation of rhizospheric and roots endophytic actinomycetes from Leguminosae plant and their activities to inhibit soybean pathogen, Xanthomonas campestris pv. glycine

Mingma, Ratchanee; Pathom-aree, Wasu; Trakulnaleamsai, Savitr; Thamchaipenet, Arinthip; Duangmal, Kannika
January 2014
World Journal of Microbiology & Biotechnology;Jan2014, Vol. 30 Issue 1, p271
Academic Journal
In this study, actinomycetes from roots and rhizospheric soils of leguminous plants were isolated using starch casein agar supplemented with antifungal and antibacterial antibiotics. Three hundred and seventeen actinomycetes were isolated with 77 isolates obtained from plant roots and 240 isolates from rhizospheric soils. Analysis of whole-organism hydrolysates showed that 289 strains were rich in the LL-isomer of diaminopimelic acid, a result consistent with their assignment to the streptomycetes. The remaining 28 strains were assigned to non-streptomycetes based on the presence of meso-isomer of diaminopimelic acid in cell wall. Sixty-four isolates (20.2 %) showed antagonistic activity against soybean pathogen Xanthomonas campestris pv. glycine by agar overlay method. Isolate RM 365 showed the highest activity with an inhibition ratio of 3.79, with no inhibitory activity on the growth of Rhizobium japonicum TISTR 079, Rhizobium sp. TISTR 061 and Rhizobium sp. TISTR 063. The 16S rRNA gene sequence analysis revealed that isolate RM 365 shared 99.28 % similarity to Streptomyces caeruleatus GIMN4 (GQ329712). In addition, isolates which contained meso-DAP were also identified by 16S rRNA gene sequence analysis. The results showed that they were members of the genus Amycolatopsis, Isoptericola, Micromonospora, Microbispora, Nocardia, Nonomuraea, Promicromonospora and Pseudonocardia.


Related Articles

  • Legume Root Symbioses: Natural History and Prospects for Improvement. Shtark, Oksana; Provorov, Nikolay; Mikić, Aleksandar; Borisov, Alexey; Ćupina, Branko; Tikhonovich, Igor // Field & Vegetable Crops Research / Ratarstvo i povrtarstvo;2011, Vol. 48 Issue 2, p291 

    Legumes develop different mutually beneficial microbial-root symbioses such as arbuscular mysorrhiza (AM), rhizobium-legume symbiosis (RLS) and epiphytic or endophytic associations with plant growth-promoting bacteria (PGPB) which are distinguished in level of integration of the partners....

  • Scout beans for summer insects. JOHNSON, MARK // Wallaces Farmer;Jul2014, Vol. 139 Issue 7, p35 

    The article offers the author's insights on two soybean pests in Iowa such as soybean aphids and Japanese beetles.

  • Study: resistant plants better than sprays. GRAY, MIKE // Prairie Farmer;Aug2013, Vol. 185 Issue 8, p52 

    The article focuses on the findings of the study conducted by entomologist Chris DiFonzo at Michigan State University on the favor of mild temperatures in summer to soybean aphid development and the unimportance of coupling insecticide with resistance.

  • SCN, other ills gang up.  // Wallaces Farmer;Oct2014, Vol. 139 Issue 10, p20 

    The article reports on the study conducted by Iowa State University (ISU) researchers Mike McCarville, Matt O'Neal and Greg Tylka which found that soybean aphid populations can increase the susceptibility of plants to soybean cyst nematode (SCN).

  • Elicitation of systemic resistance and growth promotion of Arabidopsis thaliana by PGPRs from Nicotiana glauca: a study of the putative induction pathway. Domenech, J.; Ramos, S. B.; Probanza, A.; Lucas, G. J. A.; Gutierrez, M. F. J. // Plant & Soil;Jan2007, Vol. 290 Issue 1/2, p43 

    The ability of six putative plant growth promoting rhizobacteria, isolated from the rhizosphere of Nicotiana glauca L., to stimulate growth and induce systemic resistance against Xanthomonas campestris CECT 95 in Arabidopsis thaliana L. Col-0 was evaluated. The six bacterial strains...

  • Amycolatopsis jiangsuensis sp. nov., a novel endophytic actinomycete isolated from a coastal plant in Jiangsu, China. Xing, Ke; Liu, Wei; Zhang, Yue-Ji; Bian, Guang-Kai; Zhang, Wen-Di; Tamura, Tomohiko; Lee, Jung-Sook; Qin, Sheng; Jiang, Ji-Hong // Antonie van Leeuwenhoek;Feb2013, Vol. 103 Issue 2, p433 

    A novel actinomycete, designated strain KLBMP 1262, was isolated from a coastal plant Dendranthema indicum (Linn.) Des Moul collected from the coastal region of Nantong, Jiangsu Province, in east China and was studied in detail for its taxonomic position. Phylogenetic analysis based on the 16S...

  • Distribution of Micromonospora Isolated from Sugar Cane in Thailand. Sinma, K.; Khucharoenphaisan, K. // Journal of Applied Sciences;2014, Vol. 14 Issue 22, p3013 

    Micromonospora is one of actinomycetes belonging to Gram-positive bacteria. It was found both inside plant tissues and outside tissues as free-living bacteria. The objectives of this study were determined the distribution of Micromonospora in sugar cane tissues; root, leave and stem which...

  • Visualization of grapevine root colonization by the Saharan soil isolate Saccharothrix algeriensis NRRL B-24137 using DOPE-FISH microscopy. Compant, Stéphane; Muzammil, Saima; Lebrihi, Ahmed; Mathieu, Florence // Plant & Soil;Sep2013, Vol. 370 Issue 1/2, p583 

    Background and aim: There is currently a gap of knowledge regarding whether some beneficial bacteria isolated from desert soils can colonize epi- and endophytically plants of temperate regions. In this study, the early steps of the colonization process of one of these bacteria, Saccharothrix...

  • RNA-Seq reveals a xenobiotic stress response in the soybean aphid, Aphis glycines, when fed aphid-resistant soybean. Bansal, Raman; Mian, MAR; Mittapalli, Omprakash; Michel, Andy P. // BMC Genomics;2014, Vol. 15 Issue 1, p1 

    Background While much recent research has expanded our understanding of the molecular interactions between aphids and their host plants, it is lacking for the soybean aphid, Aphis glycines. Since its North American invasion, A. glycines has become one of the most damaging insect pests on this...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics