TITLE

Cloning and characterization of two allelic glyceraldehyde-3-phosphate dehydrogenase genes in Auricularia auricula- judae

AUTHOR(S)
Fan, Xiuzhi; Zhou, Yan; Xiao, Yang; Bian, Yinbing
PUB. DATE
January 2014
SOURCE
World Journal of Microbiology & Biotechnology;Jan2014, Vol. 30 Issue 1, p181
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Two allelic variants of the gpd gene, Gpd and Gpd, were isolated based on a putative glyceraldehyde-3-phosphate dehydrogenase encoding sequence from the transcriptome of Auricularia auricula- judae strain Au916. The two alleles were found to have a 73 bp length discrepancy and 39 SNP variations. Both of the genomic DNA sequences of two alleles were interrupted by five introns, and encoded a same 340 aa protein. Intron positions analysis showed that the first intron was absent, but the last unique intron was gained in A. auricula- judae. Allele-specific expression analysis showed that the Gpd and Gpd were expressed with no significant difference in dikaryotic mycelia of A. auricula- judae. To the best of our knowledge, this is the first report about the detection of two allelic gpd genes in A. auricula- judae, as well as the application of allele-specific primers in gene expression analysis for this edible fungus.
ACCESSION #
93434687

 

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