Molecular characterization of a short peptidoglycan recognition protein (PGRP-S) from Asian corn borer ( Ostrinia furnacalis) and its role in triggering proPO activity

Sun, Qiang; Xu, Xiao-Xia; Freed, Shoaib; Huang, Wan-Jun; Zheng, Zhihua; Wang, Shuang; Ren, Shun-Xiang; Jin, Feng-Liang
January 2014
World Journal of Microbiology & Biotechnology;Jan2014, Vol. 30 Issue 1, p263
Academic Journal
Peptidoglycan recognition proteins (PGRPs) are non-specific immune molecules of insects, and vertebrates etc., but are not present in plants and nematodes. In the current experiment, a PGRP DNA sequence (2,910 bp containing four exons) was identified from genomic DNA library of Asian corn borer, Ostrinia furnacalis, and a full-length cDNA programming PGRP was cloned (designed as OfPGRP-S) with an open reading frame of 579 bp, having 192 amino acid. This inferred amino acid sequence showed maximum similarity to known lepidopteran PGRPs. Quantitative real-time PCR investigation disclosed the level of mRNA of OfPGRP-S to be constitutively expressed in the whole developmental stages and with higher expression in the mature larvae. Even more the OfPGRP-S was mainly expressed in immune capable organs i.e., fat body and midgut, and was strongly induced by injecting gram-positive bacteria i.e., Staphylococus aureus. Recombinant protein OfPGRP-S could bind to S. aureus and Bacillus thuringiensis which enhance proPO activation in the presence of these microbes. The results indicated that OfPGRP-S is an inducible protein acting as a receptor-type PGRP for enhancing the proPO activation on exposure to bacteria.


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