TITLE

Investigation on the mechanisms for biotransformation of saponins to diosgenin

AUTHOR(S)
Zhu, Yuling; Zhu, Hancan; Qiu, Muqing; Zhu, Tingting; Ni, Jinren
PUB. DATE
January 2014
SOURCE
World Journal of Microbiology & Biotechnology;Jan2014, Vol. 30 Issue 1, p143
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
In order to improve the efficiency of biotransformation of saponins in Dioscorea zingiberensis to diosgenin, a new enzymatic model was developed to investigate the mechanism of the metabolic systems. Four main saponin hydrolases (E1, E2, E3 and E4) were purified from Trichoderma reesei. Using progracillin as substrate, the enzymatic hydrolysis experiments with E1, E2, E3 and E4 were carried out respectively. Saponin concentrations during each biotransformation reaction were constructed with a kinetic model consisting of a few Michaelis-Menten equations. During biotransformation, C-26 glycoside and C-3 terminal glycoside were cleaved sequentially from saponins by E1, E2, E3 and E4. Then C-3 terminal rhamnoside and C-3 glycoside were released from the aglycone stepwisely by E2 and E3, to yield diosgenin. E2 and E3 were the key enzymes in the system, and cleavage of the C-3 glycoside from saponins was the rate-limiting step in the biotransformation process. The proposed enzymatic model might be used to analyze the mechanism for biotransformation of saponins to diosgenin.
ACCESSION #
93434685

 

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