TITLE

Production of the Phanerochaete flavido- alba laccase in Aspergillus niger for synthetic dyes decolorization and biotransformation

AUTHOR(S)
Benghazi, Lamiae; Record, Eric; Suárez, Antonio; Gomez-Vidal, José; Martínez, José; Rubia, Teresa
PUB. DATE
January 2014
SOURCE
World Journal of Microbiology & Biotechnology;Jan2014, Vol. 30 Issue 1, p201
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We investigated the expression of Phanerochaete flavido- alba laccase gene in Aspergillus niger and the physical and biochemical properties of the recombinant enzyme (rLac-LPFA) in order to test it for synthetic dye biotransformation. A. niger was able to produce high levels of active recombinant enzyme (30 mgL), whose identity was further confirmed by immunodetection using Western blot analysis and N-terminal sequencing. Interestingly, rLac-LPFA exhibited an improved stability at pH (2-9) and organic solvents tested. Furthermore, the percentage of decoloration and biotransformation of synthetic textile dyes, Remazol Brilliant Blue R (RBBR) and Acid Red 299 (NY1), was higher than for the native enzyme. Its high production, simple purification, high activity, stability and ability to transform textile dyes make rLac-LPFA a good candidate for industrial applications.
ACCESSION #
93434681

 

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