Production of the Phanerochaete flavido- alba laccase in Aspergillus niger for synthetic dyes decolorization and biotransformation

Benghazi, Lamiae; Record, Eric; Suárez, Antonio; Gomez-Vidal, José; Martínez, José; Rubia, Teresa
January 2014
World Journal of Microbiology & Biotechnology;Jan2014, Vol. 30 Issue 1, p201
Academic Journal
We investigated the expression of Phanerochaete flavido- alba laccase gene in Aspergillus niger and the physical and biochemical properties of the recombinant enzyme (rLac-LPFA) in order to test it for synthetic dye biotransformation. A. niger was able to produce high levels of active recombinant enzyme (30 mgL), whose identity was further confirmed by immunodetection using Western blot analysis and N-terminal sequencing. Interestingly, rLac-LPFA exhibited an improved stability at pH (2-9) and organic solvents tested. Furthermore, the percentage of decoloration and biotransformation of synthetic textile dyes, Remazol Brilliant Blue R (RBBR) and Acid Red 299 (NY1), was higher than for the native enzyme. Its high production, simple purification, high activity, stability and ability to transform textile dyes make rLac-LPFA a good candidate for industrial applications.


Related Articles

  • Decolorization of structurally different textile dyes by Aspergillus niger SA1. Naeem Ali; Abdul Hameed; Safia Ahmed; Abdul Khan // World Journal of Microbiology & Biotechnology;Jul2008, Vol. 24 Issue 7, p1067 

    Abstract  The fungal strain, Aspergillus niger SA1, isolated from textile wastewater sludge was screened for its decolorization ability for four different textile dyes. It was initially adapted to higher concentration of dyes (10–1,000 mg l−1) on solid culture medium...

  • Direct-dyes bioremoval using Aspergillus niger in pilot scale bioreactor. El-Rahim, Wafaa M. Abd; El-Ardy, Ola Ahmed M. // Desalination & Water Treatment;Jan2011, Vol. 25 Issue 1-3, p39 

    Treatment of effluents from dye-based industries represents one of the most important approaches to protect the surrounding agricultural environment from heavy pollutions particularly in developing countries. In this work, decolorization of two structurally different direct textile dyes; Direct...

  • Enhanced decolourisation ability of laccase towards various synthetic dyes by an electrocatalysis technology. Cameselle, C.; Pazos, M.; Lorenzo, M.; Sanromán, MA. // Biotechnology Letters;Apr2003, Vol. 25 Issue 8, p603 

    Laccase in culture filtrates of Trametes versicolor degraded a number of structurally different dyes by about 30% after 30 min though only 5% of Azure B was degraded in 1 h and Poly R-478 and fuchsin were not degraded at all even after 24 h. However, by using enzymatic electrocatalysis...

  • Comparative study of biochemical properties of glucoamylases from the filamentous fungi Penicillium and Aspergillus. Volkov, P.; Rozhkova, A.; Semenova, M.; Zorov, I.; Sinitsyn, A. // Biochemistry (00062979);Oct2013, Vol. 78 Issue 10, p1180 

    Here we report the first isolation to homogeneous forms of two glucoamylases from the fungus Penicillium verruculosum and their study in comparison with known glucoamylases from Aspergillus awamori and Aspergillus niger. Genes that encode glucoamylases from P. verruculosum were cloned and...

  • Characterization of metabolites produced from the biotransformation of 6-shogaol formed by Aspergillus niger. Jo, Soo; Kim, In; Rehman, Shaheed; Ha, Sang; Park, Ho-Young; Park, Yong; Yoo, Hye // European Food Research & Technology;Jan2016, Vol. 242 Issue 1, p137 

    6-Shogaol [(4E)-1-(4-Hydroxy-3-methoxyphenyl)-4-decen-3-one] is a pungent and bioactive ingredient of ginger. This study investigated the biotransformation of 6-shogaol by Aspergillus niger. 6-Shogaol was fermented by A. niger, and the resultant metabolites were structurally characterized by...

  • Decolourisation of reactive textile dyes Drimarene Blue X3LR and Remazol Brilliant Blue R by Funalia trogii ATCC 200800. H. Duygu Özsoy; Ali Ünyayar; M. Ali Mazmancı // Biodegradation;Jun2005, Vol. 16 Issue 3, p195 

    Abstract Decolourisation of reactive dyes Drimarene Blue X3LR and Remazol Brilliant Blue R by white rot fungi Funalia trogii was studied under static conditions. The effect of various conditions such as mycelial age, initial dye and glucose concentrations on decolourisation were also...

  • Dye decolorisation by laccase entrapped in copper alginate. Churapa Teerapatsakul; Christopher Bucke; Roberto Parra; Tajalli Keshavarz; Lerluck Chitradon // World Journal of Microbiology & Biotechnology;Aug2008, Vol. 24 Issue 8, p1367 

    Abstract  A novel immobilisation system was developed for dye decolorisation using laccase produced by Ganoderma sp. KU-Alk4. The enzyme showed high efficiency in dye decolorisation when entrapped in Cu–Al and Cu-alginate beads. The former gave the highest activity but the enzyme...

  • Decolorisation of textile dye effluent using fungal microflora isolated from spent mushroom substrate (SMS). Manikandan, N.; Kuzhali, S. Surumbar; Kumuthakalavalli, R. // Journal of Microbiology & Biotechnology Research;2012, Vol. 2 Issue 1, p57 

    Decolorisation study was carried out by treating the textile dye effluent with the fungal strains namely Aspergillus niger, Pencillium spp., Rhizopus spp isolated from spent mushroom substrate (SMS). The fungal strains were inoculated directly into the effluent, nourished with two nutritional...

  • RAPID DECOLOURISATION OF ACID RED 10B BY FUNGAL STRAINS. KRISHNAN, VEDA; SIDDIQUI, SAHIDA // Journal of Applied Technology in Environmental Sanitation;Jul2013, Vol. 3 Issue 1, p21 

    Decolourisation of Acid Red 10B by different fungal strains like Aspergillus flavus, Aspergillus niger, Chaetobium globosum, Pénicillium funiculosum, Pluerotusflabellatus, Trichodermaviride, Rhizoctoniasolani, Fusariumoxysporum, Aspergillusfoetidus and Pecillomycesvariotti were studied. Among...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics