Limited Flow Cytometry Panels on Bone Marrow Specimens Reduce Costs and Predict Negative Cytogenetics

Hoffmann, Douglas G.; Kim, Burton H.
January 2014
American Journal of Clinical Pathology;Jan2014, Vol. 141 Issue 1, p94
Academic Journal
Objectives: To determine the clinical and financial impact and predictive value of a limited flow cytometry strategy in the evaluation of bone marrow specimens. Methods: Consecutive bone marrow cases (n = 1,242) were reviewed following the independent, prospective application of two flow cytometry protocols: a limited marker strategy and a multimarker strategy. Combined morphologic and flow cytometry findings were also compared with cytogenetic results. Results: A limited flow cytometry strategy did not have a negative impact on disease detection and resulted in reduced utilization and cost. In addition, negative combined morphology and flow cytometry had a 98.4% predictive value for negative cytogenetics (P < .001). Conclusions: Careful initial evaluation of bone marrow specimens can markedly reduce the costs of bone marrow examination and significantly reduce the need for flow cytometric and cytogenetic studies on these samples.


Related Articles

  • Evaluation of canine bone marrow proliferative disorders by use of flow cytometric analysis of CD45 expression and intracytoplasmic complexity. Weiss, Douglas // Comparative Clinical Pathology;Nov2004, Vol. 13 Issue 2, p51 

    Bone marrow from 14 dogs with proliferative disorders was evaluated by labeling cells with anti-CD45 and analyzing them by use of flow cytometry. Bone marrow from five healthy dogs was also evaluated to identify normal cell labeling patterns. The cells were displayed as the logarithm of red...

  • Best Practices for Evaluation of Bone Marrow in Nonclinical Toxicity Studies. REAGAN, WILLIAM J.; IRIZARRY-ROVIRA, ARMANDO; POITOUT-BELISSENT, FLORENCE; BOLLIGER, ANNE PROVENCHER; RAMAIAH, SHASHI K.; TRAVLOS, GREG; WALKER, DANA; BOUNOUS, DENISE; WALTER, GAIL // Toxicologic Pathology;Feb2011, Vol. 39 Issue 2, p435 

    This manuscript is intended to provide a best practice approach to accurately and consistently assess toxicant-induced bone marrow effects of test articles. In nonclinical toxicity studies, complete blood count data in conjunction with the histological examination of the bone marrow are...

  • Chronic Myelomonocytic Leukemia Monocytes Uniformly Display a Population of Monocytes With CD11c Underexpression. Sojitra, Payal; Gandhi, Pranav; Fitting, Priscilla; Kini, Ameet R.; Alkan, Serhan; Velankar, Milind M.; Venkataraman, Girish // American Journal of Clinical Pathology;Nov2013, Vol. 140 Issue 5, p686 

    Objectives: To examine the utility of CD11c expression on monocytes in normal controls and patients with chronic myelomonocytic leukemia (CMML) (n = 23) with flow cytometric immunophenotyping. Methods: Twenty-three CMML samples and 10 control bone marrows submitted for lymphoma staging without...

  • Myelodysplastic Syndrome with t(1;7) Associated with Marked Dysmegakarypoiesis & Severe Thrombocytopenia: A Case Report and Review of the Literature. Gilbertson, Michael; Tuckfield, Annabel; Juneja, Surender // Case Reports in Hematology;2012, p1 

    We present the case of a 70-year-old woman who had a bone marrow examination performed to investigate marked thrombocytopenia in the context of a recent history of metastatic glucagonoma. Surprisingly this identified marked dysmegakaryopoiesis and fulfilled diagnostic criteria for refractory...

  • A Qualitative and Quantitative Analysis of Autologous Human Multipotent Adult Stem Cells Derived from Three Anatomic Areas by Marrow Aspiration: Tibia, Anterior Ilium, and Posterior Ilium. Marx, Robert E.; Tursun, Ramzey // International Journal of Oral & Maxillofacial Implants;2013, Vol. 28 Issue 5, pe290 

    Purpose: The purpose of this article was to compare the yields of stromal multipotent stem cells (CD34+ and CD105+) and hematopoetic multipotent stem cells (CD44+) obtained from different areas via bone marrow aspiration (BMA). Materials and Methods: Sixty 60-mL bone marrow aspirates were taken...

  • Diagnosis of De Novo Acute Myelogenous Leukemia With Myelodysplasia Related Changes. King, Becky // Laboratory Medicine;Spring2013, Vol. 44 Issue 2, pe29 

    Patient: 46-year-old male. Chief Complaint: Fatigue, weight loss, decreased appetite, shortness of breath. History of Present Illness: Symptom onset appeared approximately 1 month ago, but progressively worsened over time. Medical History: No known current medical problems, but has a long...

  • Relapsed T-cell acute lymphoblastic leukaemia in the vitreous of an adult: a case report. Allchin, R; Pradeep, A; Sheard, R; Rennie, I G; Mudhar, H S // Eye;Aug2014, Vol. 28 Issue 8, p1035 

    The article presents a case study of a 20-year-old African teenager suffering from lymphadenopathy and night sweats. The patient underwent flow cytometry of a bone marrow aspirate which diagnosed him of having T-cell acute lymphoblastic leukaemia (T-ALL). He also experienced painless vision...

  • An integrated nano-scale approach to profile miRNAs in limited clinical samples. Seumois, Grégory; Vijayanand, Pandurangan; Eisley, Christopher J.; Omran, Nada; Kalinke, Lukas; North, Mal; Ganesan, Asha P.; Simpson, Laura J.; Hunkapiller, Nathan; Moltzahn, Felix; Woodruff, Prescott G.; Fahy, John V.; Erle, David J.; Djukanovic, Ratko; Blelloch, Robert; Ansel, K. Mark // American Journal of Clinical & Experimental Immunology;2012, Vol. 1 Issue 2, p70 

    Profiling miRNA expression in cells that directly contribute to human disease pathogenesis is likely to aid the discovery of novel drug targets and biomarkers. However, tissue heterogeneity and the limited amount of human diseased tissue available for research purposes present fundamental...

  • High expression of wee1 is associated with malignancy in vulvar squamous cell carcinoma patients. Magnussen, Gry Irene; Hellesylt, Ellen; Nesland, Jahn M.; Trope, Claes G.; Flørenes, Vivi Ann; Holm, Ruth // BMC Cancer;2013, Vol. 13 Issue 1, p1 

    Background: Vulvar squamous cell carcinoma is a cancer form with increasing incidence rate and few treatment options. Wee1 is a central regulator of the G2/M DNA-damage checkpoint, and has in previous studies been described as a prognostic biomarker and a potential target for therapy in other...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics