Elevated MicroRNA-31 Expression Regulates Colorectal Cancer Progression by Repressing Its Target Gene SATB2

Yang, Min-Hui; Yu, Jiang; Chen, Na; Wang, Xiao-Yan; Liu, Xiang-Yu; Wang, Shuang; Ding, Yan-Qing
December 2013
PLoS ONE;Dec2013, Vol. 8 Issue 12, p1
Academic Journal
Several studies have brought about increasing evidence to support the hypothesis that miRNAs play a pivotal role in multiple processes of carcinogenesis, including cell growth, apoptosis, differentiation, and metastasis. In this study, we investigated the potential role of miR-31 in colorectal cancer (CRC) aggressiveness and its underlying mechanisms. We found that miR-31 increased in CRC cells originated from metastatic foci and human primary CRC tissues with lymph node metastases. Furthermore, the high-level expression of miR-31 was significantly associated with a more aggressive and poor prognostic phenotype of patients with CRC (p < 0.05). The stable over-expression of miR-31 in CRC cells was sufficient to promote cell proliferation, invasion, and migration in vitro. It facilitated tumor growth and metastasis in vivo too. Further studies showed that miR-31 can directly bind to the 3’untranslated region (3’UTR) of SATB2 mRNA and subsequently repress both the mRNA and protein expressions of SATB2. Ectopic expression of SATB2 by transiently transfected with pCAG-SATB2 vector encoding the entire SATB2 coding sequence could reverse the effects of miR-31 on CRC tumorigenesis and progression. In addition, ectopic over-expression of miR-31 in CRC cells induced epithelial-mesenchymal transition (EMT). Our results illustrated that the up-regulation of miR-31 played an important role in CRC cell proliferation, invasion, and metastasis in vitro and in vivo through direct repressing SATB2, suggesting a potential application of miR-31 in prognosis prediction and therapeutic application in CRC.


Related Articles

  • Identification of Unique miRNA Biomarkers in Colorectal Adenoma and Carcinoma Using Microarray: Evaluation of Their Putative Role in Disease Progression. Narasimhan, Kothandaraman; Gauthaman, Kalamegam; Pushparaj, Peter Natesan; Meenakumari, Govindasamy; Ahmed Chaudhary, Adeel Gulzar; Abuzenadah, Adel; Gari, Mamdooh Abdullah; Al Qahtani, Mohammed; Manikandan, Jayapal // ISRN Otolaryngology;2014, p1 

    MicroRNAs (miRNAs) are known to be dysregulated and play a key role in cancer progression. The present study aims to identify the miRNAs associated with colorectal adenoma and carcinoma to evaluate their role in tumor progression and metastasis using microarray. In silico analysis of miRNAs was...

  • MicroRNA Profiling in the Malignant Progression of Gliomas. Stupak, E. V.; Veryaskina, Yu. A.; Titov, S. E.; Achmerova, L. G.; Stupak, V. V.; Ivanov, M. K.; Zhimulev, I. F.; Kolesnikov, N. N. // AIP Conference Proceedings;2016, Vol. 1760 Issue 1, p1 

    Wealth of data indicates that microRNAs (miRNAs) are directly involved in carcinogenesis and that miRNA can, on their own, act as diagnostic and prognostic markers for various types of cancers, including gliomas. The aim of this study was to conduct a comparative analysis of expression profile...

  • Knockdown of Mir-135b Sensitizes Colorectal Cancer Cells to Oxaliplatin-Induced Apoptosis Through Increase of FOXO1. Qin, Yan; Li, Longhai; Wang, Fang; Zhou, Xinyi; Liu, Yankui; Qi, Xiaowei; Yin, Yuan // Cellular Physiology & Biochemistry (Karger AG);Aug2018, Vol. 48 Issue 4, p1628 

    Background/Aims: Aberrant expression of microRNAs (miRNAs) is found to be responsible for tumorigenesis, cancer development and chemoresistance. Although oxaliplatin is an effective chemotherapeutic drug for treatment of colorectal cancer (CRC), CRC cells can develop some mechanisms...

  • EVI1 oncogene promotes KRAS pathway through suppression of microRNA-96 in pancreatic carcinogenesis. Tanaka, M; Suzuki, H I; Shibahara, J; Kunita, A; Isagawa, T; Yoshimi, A; Kurokawa, M; Miyazono, K; Aburatani, H; Ishikawa, S; Fukayama, M // Oncogene;5/8/2014, Vol. 33 Issue 19, p2454 

    Despite frequent KRAS mutation, the early molecular mechanisms of pancreatic ductal adenocarcinoma (PDAC) development have not been fully elucidated. By tracking a potential regulator of another feature of PDAC precursors, acquisition of foregut or gastric epithelial gene signature, we herein...

  • Involvement of miR-20a in Promoting Gastric Cancer Progression by Targeting Early Growth Response 2 (EGR2). Xiangsheng Li; Zhichao Zhang; Ming Yu; Liqi Li; Guangsheng Du; Weidong Xiao; Hua Yang // International Journal of Molecular Sciences;Aug2013, Vol. 14 Issue 8, p16226 

    Gastric cancer (GC) is one of the most common cancers, with high incidences in East Asia. microRNAs (miRNAs) play essential roles in the carcinogenesis of GC. miR-20a was elevated in GC, while the potential function of miR-20a was poorly understood. miR-20a expression was examined in GC tissues...

  • Tetraspanin TSPAN12 regulates tumor growth and metastasis and inhibits β-catenin degradation. Knoblich, Konstantin; Wang, Hong-Xing; Sharma, Chandan; Fletcher, Anne; Turley, Shannon; Hemler, Martin // Cellular & Molecular Life Sciences;Apr2014, Vol. 71 Issue 7, p1305 

    Ablation of tetraspanin protein TSPAN12 from human MDA-MB-231 cells significantly decreased primary tumor xenograft growth, while increasing tumor apoptosis. Furthermore, TSPAN12 removal markedly enhanced tumor-endothelial interactions and increased metastasis to mouse lungs. TSPAN12 removal...

  • MicroRNA miR-30 family regulates non-attachment growth of breast cancer cells. Ouzounova, Maria; Tri Vuong; Ancey, Pierre-Benoit; Ferrand, Mylène; Durand, Geoffroy; Le-Calvez Kelm, Florence; Croce, Carlo; Matar, Chantal; Herceg, Zdenko; Hernandez-Vargas, Hector // BMC Genomics;2013, Vol. 14 Issue 1, p1 

    Background: A subset of breast cancer cells displays increased ability to self-renew and reproduce breast cancer heterogeneity. The characterization of these so-called putative breast tumor-initiating cells (BT-ICs) may open the road for novel therapeutic strategies. As microRNAs (miRNAs)...

  • MicroRNA-429 Modulates Hepatocellular Carcinoma Prognosis and Tumorigenesis. Xiao-Ying Huang; Jin-Guang Yao; Hong-Dong Huang; Chao Wang; Yun Ma; Qiang Xia; Xi-Dai Long // Gastroenterology Research & Practice;2013, p1 

    MicroRNA-429 (miR-429) may modify the development and progression of cancers; however, the role of this microRNA in the hepatocellular carcinoma (HCC) has not been well elaborated. Here, we tested miR-429 expression in 138 pathology-diagnosed HCC cases and SMMC-7721 cells. We found that miR-429...

  • Non-Steroidal Anti-inflammatory Drugs Decrease E2F1 Expression and Inhibit Cell Growth in Ovarian Cancer Cells. Valle, Blanca L.; D'Souza, Theresa; Becker, Kevin G.; Wood III, William H.; Zhang, Yongqing; Wersto, Robert P.; Morin, Patrice J. // PLoS ONE;Apr2013, Vol. 8 Issue 4, p1 

    Epidemiological studies have shown that the regular use of non-steroidal anti-inflammatory (NSAIDs) drugs is associated with a reduced risk of various cancers. In addition, in vitro and experiments in mouse models have demonstrated that NSAIDs decrease tumor initiation and/or progression of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics