TITLE

Functional Analysis of the Interdependence between DNA Uptake Sequence and Its Cognate ComP Receptor during Natural Transformation in Neisseria Species

AUTHOR(S)
Berry, Jamie-Lee; Cehovin, Ana; McDowell, Melanie A.; Lea, Susan M.; Pelicic, Vladimir
PUB. DATE
December 2013
SOURCE
PLoS Genetics;Dec2013, Vol. 9 Issue 12, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Natural transformation is the widespread biological process by which “competent” bacteria take up free DNA, incorporate it into their genomes, and become genetically altered or “transformed”. To curb often deleterious transformation by foreign DNA, several competent species preferentially take up their own DNA that contains specific DUS (DNA uptake sequence) watermarks. Our recent finding that ComP is the long sought DUS receptor in Neisseria species paves the way for the functional analysis of the DUS-ComP interdependence which is reported here. By abolishing/modulating ComP levels in Neisseria meningitidis, we show that the enhancement of transformation seen in the presence of DUS is entirely dependent on ComP, which also controls transformation in the absence of DUS. While peripheral bases in the DUS were found to be less important, inner bases are essential since single base mutations led to dramatically impaired interaction with ComP and transformation. Strikingly, naturally occurring DUS variants in the genomes of human Neisseria commensals differing from DUS by only one or two bases were found to be similarly impaired for transformation of N. meningitidis. By showing that ComPsub from the N. subflava commensal specifically binds its cognate DUS variant and mediates DUS-enhanced transformation when expressed in a comP mutant of N. meningitidis, we confirm that a similar mechanism is used by all Neisseria species to promote transformation by their own, or closely related DNA. Together, these findings shed new light on the molecular events involved in the earliest step in natural transformation, and reveal an elegant mechanism for modulating horizontal gene transfer between competent species sharing the same niche.
ACCESSION #
93395221

 

Related Articles

  • Nucleotide sequence analyses of genomic RNAs of Peanut stunt virus Mi, the type strain representative of a novel PSV subgroup from China. Yan, L. Y.; Xu, Z. Y.; Goldbach, R.; Kunrong, C.; Prins, M. // Archives of Virology;Jun2005, Vol. 150 Issue 6, p1203 

    The complete nucleotide sequence of Peanut stunt virus strain Mi (PSV-Mi) from China was determined and compared to other viruses of the genus Cucumovirus. The tripartite genome of PSV-Mi encoded five open reading frames (ORFs) typical of cucumoviruses. Distance analyses of four ORFs indicated...

  • TileQC: A system for tile-based quality control of Solexa data. Dolan, Peter C.; Denver, Dee R. // BMC Bioinformatics;2008, Vol. 9, Special section p1 

    Background: Next-generation DNA sequencing technologies such as Illumina's Solexa platform and Roche's 454 approach provide new avenues for investigating genome-scale questions. However, they also present novel analytical challenges that must be met for their effective application to biological...

  • Nanopore sensors for nucleic acid analysis. Venkatesan, Bala Murali; Bashir, Rashid // Nature Nanotechnology;Oct2011, Vol. 6 Issue 10, p615 

    Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged...

  • DNA Sequencing by Hybridization and Shotgun Sequencing Technique. Kumar Sahu, Narayan; Kumar Dewangan, Somesh // International Journal of Advanced Research in Computer Science;Nov/Dec2012, Vol. 3 Issue 6, p224 

    DNA sequencing includes several methods and technologies that are used for determining the order of the nucleotide bases adenine, guanine, cytosine, and thymine in a molecule of DNA. Knowledge of DNA sequences has become indispensable for basic biological research, other research branches...

  • From Genome Sequence to Genome Understanding. Lombardi, Steve // PharmaGenomics;Sep2004 Supplement, Vol. 4, p25 

    This article describes the manufacturing methods and probe set strategies required to develop microarrays that can be used successfully in these experiments. Inexpensive, reliable and automated DNA sequencing methods allowed scientists to sequence the complete genomes of organisms, ranging from...

  • Complete nucleotide sequences of the small and medium RNA genome segments of Kairi virus (family Bunyaviridae). Soto, Victor; Dorman, Karin S.; Miller, W. Allen; Farfan-Ale, Jose A.; Loroño-Pino, Maria A.; Garcia-Rejon, Julian E.; Blitvich, Bradley J. // Archives of Virology;Sep2009, Vol. 154 Issue 9, p1555 

    We determined the complete nucleotide sequences of the small (S) and medium (M) RNA genome segments of a Kairi virus (KRIV) isolate from the Yucatan Peninsula of Mexico. The S segment consists of 992 nucleotides, and the M segment consists of 4,619 nucleotides. Phylogenetic analyses were...

  • Detection of splicing events and multiread locations from RNA-seq data based on a geometric-tail (GT) distribution of intron length. Shao-Ke Lou; Jing-Woei Li; Hao Qin; Aldrin Kay-Yuen Yim; Leung-Yau Lo; Bing Ni; Kwong-Sak Leung; Stephen Kwok-Wing Tsui; Ting-Fung Chan // BMC Bioinformatics;2011 Supplement 5, Vol. 12 Issue Suppl 5, p1 

    Background: RNA sequencing (RNA-seq) measures gene expression levels and permits splicing analysis. Many existing aligners are capable of mapping millions of sequencing reads onto a reference genome. For reads that can be mapped to multiple positions along the reference genome (multireads),...

  • Modelling the evolution of multi-gene families. Nye, Tom M. W. // Statistical Methods in Medical Research;Oct2009, Vol. 18 Issue 5, p487 

    A number of biological processes can lead to genes being copied within the genome of some given species. Duplicate genes of this form are called paralogs and such genes share a high degree sequence similarity as well as often having closely related functions. Some genes have become widely...

  • Genome as a Two-Dimensional Walk. Larionov, S. A.; Loskutov, A. Yu.; Ryadchenko, E. V. // Doklady Physics;Dec2005, Vol. 50 Issue 12, p634 

    The article discusses the representation of a sequence of DNA nucleotides as a research approach to determine the problem of the identification and biological significance of chromosome fragments and the complete genomes. According to the study, genome refers to the complete set of the...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics